Free Radical Scavenging and Alpha/Beta-glucosidases Inhibitory Activities of Rambutan (Nephelium lappaceum L.) Peel Extract

Wahyu Widowati, Maesaroh Maesaroh, Nurul Fauziah, Pande Putu Erawijantari, Ferry Sandra

Abstract


BACKGROUND: Diabetes mellitus (DM) is associated with oxidative reaction and hyperglycemic condition. Human body has an antioxidant defense system toward free radical, but overproduction of free radical causing imbalance condition between the free radical and the antioxidant defense in the body that lead to several diseases, including DM. Glucosidase is an enzyme that hydrolize carbohydrates causing increase of blood glucose level, so by inhibiting this enzyme blood glucose level in plasma could be effectively decreased. Rambutan (Nephelium lappaceum L.) peel has been reported to have many potential roles, such as antioxidant and anti-glycemia. Therefore our current study was conducted to evaluate possible effectivity of Rambutan peel to scavenge free radical and to inhibit α- and β-glucosidases. 

METHODS:Rambutan peel extraction (RPE) was performed based on maceration method. Geraniin was used as control. For antioxidant study, 2,2-diphenyl-1- picrylhydrazyl (DPPH) free radical scavenging test was performed. For glucosidase inhibitory activity study,  α- and β-glucosidases inhibitory activity tests were performed. Results were analyzed for median of Inhibitory Concentration (IC50).

RESULTS: The scavenging activity of RPE was comparable with Geraniin. Meanwhile, the α-glucosidase inhibitory activity of RPE was higher than the one of Geraniin. The α-glucosidase-inhibitory-activity IC50 of RPE and Geraniin were 0.106±0.080 μg/ml and 16.12±0.29 μg/ml, respectively. The β-glucosidase inhibitory activity of RPE was also higher than the one of Geraniin. The β-glucosidase-inhibitory-activity IC50 of RPE and Geraniin were 7.02±0.99 μg/ml and 19.81±0.66 μg/ml, respectively.

CONCLUSION: Since RPE showed comparable free radical scavenging activity with Geraniin and higher α- and β-glucosidases inhibitory activities than Geraniin, RPE could be suggested as a promising antioxidant and antiglycemic agent. 

KEYWORDS: Nephelium lappaceum L., rambutan, hypoglycemic, antioxidant, free radical, diabetes mellitus, glucosidase, DPPH


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References


Somsak L, Naqya V, Hadady Z, Dosca T, Gergely P. Glucose analog inhibitors of glycogen phosporylases as potential antidiabetic agents: recent developments. Curr Pharm Des. 2003; 9: 1177-89, CrossRef.

Ceriello A, Motz E. Is oxidative stress the pathogenic mechanism underlying insulin resistance, diabetes, and cardiovascular disease? The common soil hypothesis revisited. Arterioscler Thromb Vasc Biol. 2004; 24: 816-23, CrossRef.

Caillet S, Lorenzo G, Côté J, Sylvain JF, Lacroix M. Free radical-scavenging properties and antioxidant activity of fractions from cranberry products. FNS. 2012; 3: 337-47, CrossRef.

Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007; 39: 44-84, CrossRef.

Bahoran T, Soobrattee MA, Luximon-Ramma V, Aruoma OI. Free radicals and antioxidants in cardiovascular health and disease. IJMU. 2006; 1: 25-41, CrossRef.

Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impacts on human health. Pharmacogn Rev. 2010; 4: 118-26, CrossRef.

Mayne ST. Antioxidant nutrients and chronic disease: use of biomarkers of exposure and oxidative stress status in epidemiologic research. J Nutr. 2003; 133 (Suppl 3): 933-40, PMID.

Zhao LY, Lan QJ, Huang ZC, Ouyang LJ, Zeng FH. Antidiabetic effect of a newly identified component of Opuntia dillenii polysaccharides. Phytomedicine. 2011; 18: 661-8, CrossRef.

Rendell M. The role of sulphonylureas in the management of type 2 diabetes mellitus. Drugs. 2004; 64: 1339-58, CrossRef.

Murai A, Iwamura K, Takada M, Ogawa K, Usui T, Okumura J. Control of postprandial hyperglycaemia by galactosyl maltobionolactone and its novel anti-amylase effect in mice. Life Sci. 2002; 71: 1405- 15, CrossRef.

Lebovitz H. Alpha-Glucosidase inhibitors. Endocrinol Metab Clin North Am. 1997; 26: 539-51, CrossRef.

Kumar S, Narwal S, Kumar V, Prakash O. Alpha-glucosidase inhibitors from plants: A natural approach to treat diabetes. Pharmacogn Rev. 2011; 5: 19-29, CrossRef.

Mukherjee PK, Maiti K, Mukherjee K, Houghton PJ. Leads from Indian medicinal plants with hypoglycemic potentials. J Ethnopharmacol. 2006; 106: 1-28, CrossRef.

Palanisamy U, Cheng HM, Masilamani T, Subramaniam T, Ling LT, Radhakrishna AK. Rind of rambutan, Nephelium lappaceum, a potential source of natural antioxidants. Food Chem. 2008; 109: 54-63, CrossRef.

Widowati W, Mozef T, Risdian C, Ratnawati H, Tjahjani S, Sandra F. The comparison of antioxidative and proliferation inhibitor properties of Piper betle L., Catharanthus roseus [L] G.Don, Dendrophtoe petandra L., Curcuma mangga Val. Extracts on T47D Cancer Cell Line. Int Res J Biochem Bioinform. 2011; 1: 22-8, article.

Widowati W, Sardjono CT, Wijaya L, Laksmitawati DR, Sandra F. Extract of Curcuma longa L. and (-)-Epigallo Cathechin-3-Gallate enhanced proliferation of adipose tissue-derived mesenchymal stem cells (AD-MSCs) and differentiation of AD-MSCs into endothelial progenitor cells. J US China Med Sci. 2012; 9: 22-9, article.

Widowati W, Ratnawati H, Rusdi DU, Winarno W, Kasim F. The antiplatelet aggregation effect of extract and ethyl acetate fraction of velvet bean seed (Mucuna pruriens L.) in dyslipidemic rat. Agritech. 2011; 31: 52-9, article.

Widowati W, Mozef T, Risdian C, Ratnawati H, Tjahyani S, Sandra F. Apoptosis and Antioxidant Activities of Catharanthus roseus [L] G. Don Extrcat on Breast Cancer Cell Line. Indones J Cancer Chemoprevent. 2010; 1: 99-107, article.

Widowati W, Widyanto RM, Laksmitawati DR, Erawijantari PP, Wijaya L, Sandra F. Phytochemical, Free Radical Scavenging and Cytotoxic Assay of Cucumis Melo L. Extract and β-Carotene. J Adv Agric Technol. 2015; 2: 114-9, CrossRef.

Widowati W, Wijaya L, Wargasetia TL, Yelliantty Y, Laksmitawati DR. Antioxidant, anticancer, and apoptosis-inducing effects of Piper extracts in HeLa cells. J Exp Integr Med. 2013; 3: 225-30, CrossRef.

Widowati W, Herlina T, Ratnawati H, Mozef T, Risdian C. Antioxidant and platelet aggregation inhibitor activities of black tea (Camellia sinensis L.) extract and fractions. Med Plants. 2011; 3: 21-6, CrossRef.

Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol. 2004; 26: 211-9, article.

Kim YM, Wang MH, Rhee HI. A novel a-glucosidase inhibitor from pine bark. Carbohydr Res. 2004; 339: 715-7, CrossRef.

Widowati W, Ratnawati H, Retnaningsih CH, Lindayani, Rusdi DU, Winarno W. Free radical scavenging and a-glucosidase inhibitor activity of ethanolic extract of Mucuna pruriens L. JFI. 2011; 5: 117-24, article.

Samuagam L, Sia CM, Akouwah GA, Okechukwu PN, Yim HS. The effect of extraction condition on total phenolic content and free radical scavenging capacity of selected tropical fruits’ peel. HEJ. 2013; 4: 80-102, article.

Thitilertdecha N, Teerawutgulrag A, Rakariyatham N. Antioxidant and antibacterial activities of Nepheliumlappaceum L. extracts. LWT-Food Sci Technol. 2008; 41: 2029-35, CrossRef.

Palanisamy U, Ling LT, Manaharan T, Appleton D. Rapid isolation of geraniin from Nepheliumlappaceum rind waste and its anti-hyperglycemic activity. Food Chem. 2011; 127: 21-7, CrossRef.

Manaharan T, Palanisamy UD, Ming CH. Tropical Plant extracts as potential antihyperglycemic agents. Molecules. 2012; 17: 5915-23, CrossRef.

Tachakittirungrod S, Okonogi S, Chowwanapoonpohn S. Study of antioxidant activity of certain plants in Thailand: mechanism of antioxidant action of guava leaf extract. Food Chem. 2007; 103: 381-8, CrossRef.

Pandey S, Sree A, Dash SS, Sethi DP. A novel method for screening beta-glucosidase inhibitors. BMC Microbiol. 2013; 13: 55, CrossRef.

Sancheti S, Sancheti S, Seo SY. Chaenomeles Sinensis: A potent α- and β-glucosidase inhibitor, Am J Pharmacol Toxicol. 2009; 4 : 8-11, CrossRef.

Toller M. Alpha-Glucosidase inhibitors in diabetes: efficacy in NIDDM subjects. Euro J Clin Invest. 1994; 24 (Suppl 3): 31-5, CrossRef.

Palanisamy U, Manaharan T, Teng LL, Radhakrishnan AKC, Subramainiam T, Masilamani T. Rambutan rind in the management of hyperglycemia. Food Res Int. 2011; 44: 2278-82, CrossRef.

LeRoith D, Taylor SI, Olefsky JM. Diabetes Mellitus: a fundamental and clinical text. 3rd ed. Philadelphia: Lippincott Williams and Wilkins; 2004, NLMID.




DOI: https://doi.org/10.18585/inabj.v7i3.180

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