High NF-κB and RAGE Expression in Fetal Membrane of Premature Rupture of Membrane (PROM) Subject

I Ketut Edy Sudiarta, Monicha Zalzabilla Aldinasyah, Cindy Jennilyn Candra, Supriyono Supriyono, Annisa Ullya Rasyida

Abstract


BACKGROUND: Premature Rupture of Membranes (PROM) is significantly linked to the infections-related maternal deaths. In the inflammatory process, the influencing stressor will stimulate the activation of Nuclear Factor Kappa B (NF-κB) and Receptor of Advanced Gyclation End product (RAGE). Yet up to date, the expression of NF-κB and RAGE in pregnant women with PROM are still rarely studied. Therefore, this study aimed to observe the differences of NF-κB and RAGE expression from PROM and non-PROM subjects.

METHODS: This was a cross-sectional study involving 20 PROM subjects and 20 non-PROM subjects with infections and complications. Samples from the fetal membrane tissue of subjects were obtained and put into paraffin block preparation for the determination of NF-κB and RAGE expression. The detection of NF-κB and RAGE expression was conducted using immunohistochemical staining and observed under an upright light microscope. The expressions were later calculated using ImageJ software.

RESULTS: Both NF-κB and RAGE expression were found to be higher in PROM subjects compare to the non-PROM subjects. The median of NF-κB in PROM and non-PROM subjects were 32.47±1.22 and 5.59±1.09, respectively (p=0.000). While the median of RAGE in PROM subjects was 53.58±3.46, and in non-PROM subjects was 11.64±2.49 (p=0.013).

CONCLUSION: There is significant difference between NF-κB and RAGE expression in fetal membranes of PROM and non-PROM subjects. Therefore, the increased of NF-κB and RAGE expression can be used as a potential marker to detect complication of PROM.

KEYWORDS: premature ruptures of membrane, non-premature ruptures of membrane, expression of NF-κB

 


Full Text:

PDF

References


Maryuni, Kurniasih D. Risk factors of premature rupture of membrane. Kesmas. 2017; 11(3): 133-7, CrossRef.

Judistiani RTD, Samosir SM, Irianti S, Purwara BH, Setiabudiawan B, Mose JC, et al. Correlation of maternal serum hepcidin, soluble transferrin receptor (sTfR) and cholecalciferol with third trimester anemia: findings from a nested case-control study on a pregnancy cohort. Indones Biomed J. 2020; 12(4): 361-7, CrossRef.

Kementrian Kesehatan Republik Indonesia. Profil Kesehatan Indonesia 2019. Jakarta: Kementerian Kesehatan Republik Indonesia; 2019, article.

Dinas Kesehatan Jawa Timur. Profil Kesehatan Provinsi Jawa Timur 2019. Surabaya: Dinas Kesehatan Jawa Timur; 2019, article.

Karmia HR, Afriwardi, Ali H, Mose JC, Yusrawati. The correlation of L-citrulline levels with blood pressure in severe preeclampsia. Indones Biomed J. 2020; 12(1): 15-8, article.

Akbar MIA, Sari IM, Ernawati, Aditiawarman. Plasma Level of Umbilical Cord Hemeoxygenase-1 (HO-1) and Neonatal Outcome in Early Onset and Late Onset Severe Preeclampsia. Mol Cell Biomed Sci. 2019; 3(1): 54-9, CrossRef.

Yusrawati, Habibah RL, Machmud R. Differences in maternal leptin serum levels between normal pregnancy and preeclampsia. Indones Biomed J. 2015; 7(1): 37-42, CrossRef.

Abouseif HA, Mansour AF, Hassan SF, Sabbour SM. Prevalence and outcome of preterm premature rupture of membranes (PPROM) among pregnant women attending Ain Shams Maternity Hospital. Egypt J Community Med. 2018; 36(2): 99-107, CrossRef.

Aprilla N. Faktor risiko ibu bersalin yang mengalami ketuban pecah dini di RSUD Bangkinang tahun 2017. Prepotif. 2018; 2(1): 48-57, CrossRef.

Wang F, Wang Y, Wang R, Qiu H, Chen L. Predictive value of maternal serum NF-κB p65 and sTREM-1 for subclinical chorioamnionitis in premature rupture of membranes. Am J Reprod Immunol. 2016; 76(3): 217-23, CrossRef.

Hamuaty RB, Sukmawati IR, Sandra F. Relationship between sRAGE and hsCRP as markers of cardiovascular disease risk factors in diabetic and non-diabetic men with central obesity. Mol Cell Biomed Sci. 2017; 1(2): 70-4, CrossRef.

Santangelo C, Filardi T, Perrone G, Mariani M, Mari E, Scazzocchio B, et al. Cross-talk between fetal membranes and visceral adipose tissue involves HMGB1–RAGE and VIP–VPAC2 pathways in human gestational diabetes mellitus. Acta Diabetol. 2019; 56(6): 681-9, CrossRef.

Zenerino C, Nuzzo AM, Giuffrida D, Biolcati M, Zicari A, Todros T, et al. The HMGB1/RAGE pro-inflammatory axis in the human placenta: modulating effect of low molecular weight heparin. Molecules. 2017; 22(11): 1997, CrossRef.

NFkB p50 (E-10): sc-8414. Dallas: Santa Cruz Biotechnology; [n.y] , article.

Rahaju P, Kintono RA, Wahyudiono AD, Satria A, Sandra F. Immunohistochemical expression of EGFR, NF-kB and cyclin d1 in sinonasal inverted papilloma and squamous cell carcinoma. Indones Biomed J. 2020; 12(3): 239-44, CrossRef.

Erşahin SS. The comparison of amniotic fluid nuclearfactor-kappa B levels in pregnant women who underwent cesarean section or normal vaginal labor. Perinat J. 2021; 29(1): 8-12, CrossRef.

Padron JG, Saito Reis CA, Kendal-Wright CE. The role of danger associated molecular patterns in human fetal membrane weakening. Front Physiol. 2020; 11: 602, CrossRef.

Yan H, Zhu L, Zhang Z, Li H, Li P, Wang Y, et al. HMGB1-RAGE Signaling Pathway in pPROM. Taiwan J Obstet Gynecol. 2018; 57(2): 211-6, CrossRef.

Widaningrum Y, Ernawati, Widjiati. Peluang baru pemberian kortikosteroid sebagai terapi pada kasus preeklampsia. Maj Obs Ginekol. 2014; 22(1): 22-30, article.

Santoro T, Azevedo CT, e Silva PMR, Martins MA, Carvalho VF. Glucocorticoids decrease the numbers and activation of mast cells by inducing the transactivation receptors of AGEs. J Leukoc Biol. 2019; 105(1): 131-42, CrossRef.




DOI: https://doi.org/10.18585/inabj.v14i3.1859

 

Indexed by:

                  

               

                 

 

 

The Prodia Education and Research Institute