Purple Sweet Potato Yogurt Affects Lipid Metabolism and Reduces Systemic Inflammation and Oxidative Stress in High Fat Diet Mice

Astrid Feinisa Khairani, Nur Atik, Putri Halleyana Adrikni Rahman, Enny Rohmawaty, Cynthia Noviyanti, Resti Santika, Jose Arimathea, Widad Aghnia Shalannandia

Abstract


BACKGROUND: Purple  sweet  potato  yogurt (PSPY) is a funtional food which is rich in anthocyanin and probiotics. However, the currently available data on its potentially protective effect on anthropometry, lipid metabolism, oxidative stress, and pro-inflammatory markers is very minimal, especially in mice. This study was performed to investigate those effects on balb/c mice models (Mus musculus) given a high-fat diet (HFD).

METHOD: Balb/c mice were treated with or without standard diet, HFD, ethanol extract, yogurt, and PSPY according to the group. The changes of anthropometry were analyzed using Lee Index. After three months, the interscapular brown adipose tissue (iBAT) was morphologically observed with hematoxylin and eosin (H&E) staining. The blood serum was used for evaluation using cholesterol oxidase-peroxidase aminoantypirin (CHOD-PAP) for lipid profile, enzyme-linked immunoassay (ELISA) for tumor necrosis factor (TNF)-α and interleukin (IL)-6, and thiobarbituric acid reactive substance (TBARS) procedure for malondialdehyde (MDA).

RESULT: Lee Index revealed a decrease in time (p<0.0001). The PSPY group showed a decrease in iBAT weight (p<0.05), lipid profiles including LDL (p<0.05) and total cholesterol (p>0.05), TNF-α and IL-6 (p>0.05), and MDA (p>0.05). Adipocytes’ density showed a significant increase (p=0.001). 

CONCLUSION: This research finding indicates that PSPY affects lipid metabolism and has a potential protective effect of reducing systemic inflammation and oxidative stress.

KEYWORDS: anthocyanin, high-fat diet, lee index, lipid metabolism, oxidative stress, purple sweet potato yogurt, systemic inflammation


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Nauli F, Nurhasanah, Mahati E, Bahrudin U. Body fat percentage, waist circumference and body mass index are correlated with nitric oxide levels in young adults with central obesity. Mol Cell Biomed Sci. 2021; 5(1): 1-7, CrossRef.

Wang QA, Tao C, Gupta RK, Scherer PE. Tracking adipogenesis during white adipose tissue development, expansion and regeneration. Nat Med. 2013; 19(10): 1338-44, CrossRef.

Febriza A, Ridwan, As’ad S, Kasim VN, Idrus HH. Adiponectin and its role in inflammatory process of obesity. Mol Cell Biomed Sci. 2019; 3(2): 60-6, CrossRef.

Kesh SB, Sarkar D, Manna K. High-fat diet-induced oxidative stress and its impact on metabolic syndrome : a review. Asian J Pharm Clin Res. 2016; 9(1): 47-52, article.

Sartika CR, Wijaya A, As’ad S. Pro-inflammatory profiles of indonesian adult men with central obesity: a preliminary study on TNF-alpha, sTNFR-2 and IL-1beta. Indones Biomed J. 2010; 2(1): 66-72, CrossRef.

Abdelkader RSE, El-Beih NM, Zaahkouk SA, El-Hussieny EA. Ameliorative Effect of Eruca sativa seeds and its rutin on gentamicin‑induced nephrotoxicity in male rats via targeting inflammatory status, oxidative stress and kidney injury molecule-1 (KIM-1)/cystatin c expression. Indones Biomed J. 2022; 14(1): 74-83, CrossRef.

Kim KA, Gu W, Lee IA, Joh EH, Kim DH. High fat diet-induced gut microbiota exacerbates inflammation and obesity in mice via the TLR4 signaling pathway. PLoS One. 2012; 7(10): e47713, CrossRef.

Kementrian Kesehatan. Hasil Utama Riskedas 2018. Jakarta: Kementerian Kesehatan Badan Penelitian dan Pengembangan Kesehatan; 2018, article.

World Health Organisation. Raised cholesterol. Geneva: World Health Organization; 2015, article.

Pinal-Fernandez I, Casal-Dominguez M, Mammen AL. Statins: pros and cons. Medicina Clinica. 2018; 150(10): 398-402, CrossRef.

Anyadike N. Evaluation of total antioxidant status, superoxide dismutase and malondialdehyde in apparently healthy active tobacco smokers in Nnewi Metropolis , South-East, Nigeria. J Sci Innov Res. 2017; 6(3): 105-12, CrossRef.

Utami S, Endrini S, Nafik S, Lestari IMT, Anindya D, Bakar EA, et al. In vitro antioxidant and anti-obesity activities of freeze-dried canarium sp., averrhoa bilimbi L. and malus domestica. Indones Biomed J. 2019; 11(3): 320-6, CrossRef.

Susanti E, Susilowati E. The Effect of green tea on the expression of npc1l1, abcg5, and abcg8 in the intestine of high fat diets-induced rats. Indones Biomed J. 2021; 13(2): 147-54, CrossRef.

Sun H, Zhang P, Zhu Y, Lou Q, He S. Antioxidant and prebiotic activity of five peonidin-based anthocyanins extracted from purple sweet potato (Ipomoea batatas (L.) Lam.). Sci Rep. 2018; 8(1): 5018, CrossRef.

Afiati F, Priadi G, Setiyoningrum F. The improvement of functional food in yogurt enriched with purple sweet potato (Ipomea batatas var. Ayamurasaki). J Indones Trop Anim Agric. 2018; 43(2): 159-68, article.

Pandey KR, Naik SR, Vakil B v. Probiotics, prebiotics and synbiotics- a review. J Food Sci Technol. 2015; 52(12): 7577-87, CrossRef.

Bostan K, Unver Alcay A, Yalçin S, Eren Vapur U, Nizamlioglu M. Identification and characterization of lactic acid bacteria isolated from traditional cone yoghurt. Food Sci Biotechnol. 2017; 26(6): 1625-32, CrossRef.

Wu C chen, Weng W lien, Lai W lin, Tsai H ping, Liu W hsien, Lee M hwan, et al. Effect of Lactobacillus plantarum Strain K21 on High-Fat Diet-Fed Obese Mice. Evid Based Complement Alternat Med. 2015; 2015: 391767, CrossRef.

Khairani AF, Pamela Y, Oktavia N, Achadiyani A, Adipraja MY, Zhafira PY, et al. Acute and sub-chronic oral toxicity study of purple sweet potato (Ipomoea batatas [L.] Lam) yogurt in mice (Mus musculus). Vet World. 2022; 15(3): 789-96, CrossRef.

Sameen A, Khan MI, Sattar MU, Javid A, Ayub A. Quality evaluation of yoghurt stabilized with sweet potato (Ipomoea batatas) and taro (Colocassia esculenta) starch. Int. J. Food Allied Sci. 2016; 2(1): 23-9, CrossRef.

Khairani AF, Islami U, Syamsunarno MRA, Lantika U. Synbiotic purple sweet potato yogurt ameliorate lipid metabolism in high fat diet mice model. Biomed Pharmacol J. 2020; 13(1): 175-84, CrossRef.

USDA. 2015 – 2020 Dietary Guidelines for Americans. 8th edition. Washington DC: USDA; 2015, article.

Nair AB, Jacob S. A simple practice guide for dose conversion between animals and human. J Basic Clin Pharm. 2016; 7(2): 27-31, CrossRef.

Triwitono P, Marsono Y, Murdiati A, Marseno DW. Physiological effects of mung bean starch RS-3 on the obesity index and adipose cell profile of Sprague-Dawley rats. Pak J Nutr. 2016; 15(10): 913-20, CrossRef.

Berry R , Church CD, Gericke MT, Jeffery E, Colman L, Rodeheffer MS. Imaging of adipose tissue. Methods Enzymol. 2014; 537: 47-73, CrossRef.

Jyothi L, Nuthalapati RK, Peddy SR. Prevalence of coronary artery disease and its relationship with various cardiovascular risk factors: Analysis of south Indian patients. J Indian Coll Cardiol. 2018; 8(3): 138-42, CrossRef.

World Health Organization. Obesity and overweight. World Health Organisation Media Centre Fact Sheet No. 311. Geneva: World Health Organization; 2011, article.

Chen LA, Sears CL. Prebiotics, probiotics, and synbiotics. In: Mandell, Douglas, and Bennett’s Principles and Practice of Infectious Diseases. Philadelphia: Elsevier; 2014. p.19-25, CrossRef.

Markowiak P, Ślizewska K. Effects of probiotics, prebiotics, and synbiotics on human health. Nutrients. 2017; 9(9): 1021, CrossRef.

Wallace TC, Slavin M, Frankenfeld CL. Systematic review of anthocyanins and markers of cardiovascular disease. Nutrients. 2016; 8(1): 1-13, CrossRef.

Liu C, Sun J, Lu Y, Bo Y. Effects of anthocyanin on serum lipids in dyslipidemia patients: A systematic review and meta-analysis. PLoS ONE. 2016; 11(9): 1-11, CrossRef.

Song Z, Xiaoli AM, Yang F. Regulation and metabolic significance of de novo lipogenesis in adipose tissues. Nutrients. 2018; 10(10): 1383, CrossRef.

Klingenspor M, Bast A, Bolze F, Li Y, Maurer S, Schweizer S, et al. Brown adipose tissue. In: Adipose Tissue Biology: Second Edition. Berlin: Springer; 2017. p.91-147, CrossRef.

Li J, Shi Z, Mi Y. Purple sweet potato color attenuates high fat-induced neuroinflammation in mouse brain by inhibiting MAPK and NF- κ B activation. Mol Med Rep. 2018; 17(3): 4823-31, CrossRef.

Young S, Wi H ri, Choi S, Joung T. Inhibitory effect of anthocyanin-rich black soybean testa (Glycine max (L.) Merr.) on the inflammation-induced adipogenesis in a DIO. J Funct Foods. 2015; 14(1): 623-33, CrossRef.

Kim KA, Gu W, Lee IA, Joh EH, Kim DH. High fat diet-induced gut microbiota exacerbates inflammation and obesity in mice via the tlr4 signaling pathway. 2012; 7(10): e47713, CrossRef.

Guo X, Li J, Tang R, Zhang G, Zeng H, Wood RJ, et al. High fat diet alters gut microbiota and the expression of paneth cell-antimicrobial peptides preceding changes of circulating inflammatory cytokines. Mediators Inflamm. 2017; 2017: 9474896, CrossRef.

Wu CC, Weng WL, Lai WL, Tsai HP, Liu WH, Lee MH, et al. Effect of Lactobacillus plantarum strain K21 on high-fat diet-fed obese mice. Evid Based Complement Alternat Med. 2015; 2015: 391767, CrossRef.

Prabawati RK, Ratnawati R, Rahayu M, Prakosa AG. Effect anthocyanin of purple potato gunung kawi on mda levels, expression of caspase-3, and spatial memory function on diabetic wistar rats. Malang Neurol J. 2019; 5(1): 34-41, CrossRef.

Zhao J ge, Yan Q qian, Lu L zhen, Zhang Y qing. In vivo antioxidant, hypoglycemic, and anti-tumor activities of anthocyanin extracts from purple sweet potato. Nutr Res Pract. 2013; 7(5): 359-65, CrossRef.




DOI: https://doi.org/10.18585/inabj.v14i3.1921

 

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