Evaluations of Antibacterial Properties of Zingiber purpureum Essential Oil Against 13 Different Gram-positive and Gram-negative Bacteria

Nataniel Tandirogang, Silvia Anitasari, Enos Tangke Arung, Swandari Paramita, Yung Kang Shen


BACKGROUND: Indonesia's tropical forest is home to around 80% of the world's medicinal plants. One of these is Zingiber purpureum, which have traditionally been used to treat joint discomfort, the common cold, and jaundice. The rhizomes of this plant have been suggested to possess antibacterial action in the treatment of infections. In this study, Z. purpureum was screened for antibacterial activity against 13 bacteria (Gram-positive and Gram-negative).

METHODS: Z. purpureum rhizomes were obtained and the distillated extracts were made to generate essential oil. The minimum inhibitory concentration (MIC) and Kirby Bauer disk diffusion methods were used to determine the antibacterial activity.

RESULTS: All bacteria activity were inhibited by the essential oil of Z. purpureum at concentrations ranging from 2.5 vol% to 10 vol%. However, several bacterias (Escherichia coli, Pseudomonas aeruginosa, and Enterobacter cloacae) were inhibited at the lowest concentration (0.63 vol %), with the inhibition zones ranging from 6.7 mm to 8.0 mm. Meanwhile, the widest inhibition zone (13.3 mm) was reported on E. cloacae at 10 vol% concentration.

CONCLUSION: A 10 vol% Z. purpureum rhizome extract inhibits Gram-positive and Gram-negative bacteria, particularly those that are resistant to a variety of antibiotics.

KEYWORDS: Zingiber purpureum, antibacterial agents, susceptibility test, infection

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O'Bryan CA, Pendleton SJ, Crandall PG, Ricke SC. Potential of plant essential oils and their components in animal agriculture - in vitro studies on antibacterial mode of action. Front Vet Sci. 2015; 2: 35, CrossRef.

Lim TK. Edible Medicinal and Non-medicinal Plants Vol. 10. Netherlands: Springer; 2016, NLMID.

Paramita S, Aminyoto M, Ismail S, Arung ET. Anti-hypercholesterolemic effect of Zingiber montanum extract. F1000Res. 2019; 7: 1798, CrossRef.

Anitasari S, Ismail S, Wiratama BS, Budi HS. Antibacterial and phytochemical analysis of two plants menispermaceous family. Syst Rev Pharm. 2020; 11(5): 150-6, CrossRef.

Panphut W, Budsabun T, Sangsuriya P. In vitro antimicrobial activity of Piper retrofractum fruit extracts against microbial pathogens causing infections in humans and animals. Int J Microbiol. 2020; 2020: 5638961, CrossRef.

Pithayanukul P, Tubprasert J, Wuthi-Udomlert M. In vitro antimicrobial activity of Zingiber cassumunar (Plai) oil and a 5% Plai oil gel. Phytother Res. 2007; 21(2): 164-9, CrossRef.

Patterson JE, McElmeel L, Wiederhold NP. In vitro activity of essential oils against gram-positive and gram-negative clinical isolates, including carbapenem-resistant enterobacteriaceae. Open Forum Infect. 2019; 6(12): ofz502, CrossRef.

Bučková M, Puškárová A, Kalászová V, Kisová Z, Pangallo D. Essential oils against multidrug resistant gram-negative bacteria. Biologia. 2018; 73: 803-808, CrossRef.

Semeniuc CA, Pop CR, Rotar AM. Antibacterial activity and interactions of plant essential oil combinations against Gram-positive and Gram-negative bacteria. J Food Drug Anal. 2017; 25(2): 403-8, CrossRef.

Clinical and Laboratory Standard Institute. M100-S25 Performance Standards for Antimicrobial Susceptibility Testing: Twenty-fifth Informational Supplement. Wayne: Clinical and Laboratory Standard Institute; 2015, article.

Shaheen A, Ismat F, Iqbal M, Haque A. Characterization of putative multidrug resistance transporters of the major facilitator-superfamily expressed in Salmonella typhi. J Infect Chemother. 2015; 21(5): 357-362, CrossRef.

Ifesan BOT, Ibrahim D, Voravuthikunchai SP. Antimicrobial activity of crude ethanolic extract from Eleutherine Americana. J Food Agric Environ. 2010; 8 (3/4 Part 2): 1233-6, article.

Tandirogang N, Paramita S, Yasir Y, Yuniati Y, Aminyoto M, Fitriany E. Aktivitas antimikroba ekstrak Daun Karamunting (Melastoma malabathricum L.) terhadap bakteri penyebab diare. Jurnal Sains dan Kesehatan. 2017; 1(7): 345-51, CrossRef.

Parawansah P, Nurtamin T, Mulyawati SA, Nuralifah N, Misneni WOA. Immunomodulatory effect of Momordica charantia L. fruit ethanol extract on phagocytic activity and capacity of mice peritoneal macrophages. Indones Biomed J. 2018; 10(2): 144-7, CrossRef.

Yana HY, Hidayati L, Wijayanti N, Nuringtyas TR. Immunomodulatory activity of agarwood Aquilaria malaccensis Lamk. leaf extracts on Staphylococcus aureus-infected macrophages in vitro. Indones Biomed J. 2022; 14(2): 156-63, CrossRef.

Callixte C, Damascene DJ, Ma'aruf A, Dachlan YP, Sensusuati AD, Daniel N, et al. Phytoconstituent analysis and antibacterial potential of epicarp extracts from mature fruits of Persea americana Mill. Mol Cell Biomed Sci. 2020; 4(2): 94-9, CrossRef.

Wahyuni D, Waluyo J, Prihatin J, Kusumawardani FI, Kurniawan A. Pheretima javanica K. ethanol extract Granules’ effects on eosinophil level, immunoglobulin E level, and organs histopathology in Rattus norvegicus B. Indones Biomed J. 2021; 13(2): 208-15, CrossRef.

Sudiono J, Hardina M. The effect of Myrmecodia pendans ethanol extract on inflamed pulp: study on sprague dawley rats. Mol Cell Biomed Sci. 2019; 3(2): 115-21, CrossRef.

Hyldgaard M, Mygind T, Meyer RL. Essential oils in food preservation: Mode of action, synergies, and interactions with food matrix components. Front Microbiology. 20112; 3: 12, CrossRef.

Gonelimali FD, Lin J, Miao W, Xuan J, Charles F, Chen M, et al. Antimicrobial properties and mechanism of action of some plant extracts against food pathogens and spoilage microorganisms. Front Microbiol. 2018; 9: 1639, CrossRef.

Li ZH, Cai M, Liu YS, Sun PL, Luo SL. Antibacterial activity and mechanisms of essential oil from Citrus medica L. var. sarcodactylis. Molecules. 2019; 24(8): 1577, CrossRef.

Gadisa E, Usman H. Evaluation of antibacterial activity of essential oils and their combination against multidrug-resistant bacteria isolated from skin ulcer. Int J Microbiol. 2021; 2021: 6680668, CrossRef.

Han AR, Kim H, Piao D, Jung CH, Seo EK. Phytochemicals and bioactivities of Zingiber cassumunar roxb. Molecules. 2021; 26(8): 2377, CrossRef.

Tripathi M, Chawla P, Upadhyay R, Trivedi S. Essential oils from family zingiberaceae for antimicrobial activity - a review. Int J Pharma Bio Sci. 2013; 4(4): 149-62, article.

Burt S. Essential oils: Their antibacterial properties and potential applications in foods - a review. Int J Food Microbiol. 2004; 94(3): 223-53, CrossRef.

Pius O, Oluwadunsin O, Benjamin O. Antibacterial activity of ginger (Zingiber officinale) against isolated bacteria from respiratory tract infections. J Biol Agric Healthcare. 2015; 5(19): 131-8, article.

Wahidah BF, Hayati N, Khusna UN, Rahmani TPD, Khasanah R, Kamal I, et al. The ethnobotany of zingibraceae as the traditional medicine ingredients utilized by colo muria mountain villagers, Central Java. J Phys Conf Ser. 2021; 1796: 012113, CrossRef.

Batubara I, Wahyuni WT, Susanta M. Antibacterial activity of zingiberaceae leaves essential oils against Streptococcus mutans and teeth-biofilm degradation. Int J Pharma Bio Sci. 2016; 7(4): 111-6, CrossRef.

Latifian E, Otur C, Abanoz-Secgin B, Arslanoglu SF, Kurt-Kizildogan A. Evaluation of antimicrobial activity in extracts of different parts of three tagetes species,” Turkish J Field Crops. 2021; 26: 116-22, CrossRef.

Raja SA, Ashraf M, Anjum A, Javeed A. Antibacterial activity of essential oils extracted from medicinal plants against multi-drug resistant Staphylococcus aureus. J Anim Plant Sci. 2016; 26 (2): 415-23, article.

Yusoff MM, Ibrahim H, Hamid NA. Chemical characterization and antimicrobial activity of rhizome essential oils of very closely allied zingiberaceae species endemic to Borneo: Alpinia ligulata K. SCHUM. and Alpinia nieuwenhuizii VAL. Chem Biodiver. 2011; 8(5): 916-923, CrossRef.

Widyowati R, Agil M. Chemical constituents and bioactivities of several Indonesian plants typically used in jamu. Chem Parm Bull. 2018; 66(5): 506-518, CrossRef.

Annavajhala MK, Gomez-Simmonds A, Uhlemann AC. Multidrug-resistant Enterobacter cloacae complex emerging as a global, diversifying threat. Front Microbiol. 2019; 10: 44, CrossRef.

Mezzatesta ML, Gona F, Stefani S. Enterobacter cloacae complex: Clinical impact and emerging antibiotic resistance,” Future Microbiol. 2012; 7(7): 887-902, CrossRef.

Davin-Regli A, Pagès JM. Enterobacter aerogenes and Enterobacter cloacae; versatile bacterial pathogens confronting antibiotic treatment. Front Microbiol. 2015; 6: 392, CrossRef.

Soetjipto H. Antibacterial properties of essential oil in some Indonesian herbs. In: El-Shemy, HA, editor. Potential of Essential Oils. London: IntechOpen; 2018, CrossRef.

Habsah M, Amran M, Mackeen MM, Lajis NH, Kikuzaki H, Nakatani N, Rahman AA, Ghafar, Ali AM. Screening of Zingiberaceae extracts for antimicrobial and antioxidant activities. J Ethnopharmacol. 2000; 72(3): 403-10, CrossRef.

Masuda T, Jitoe A. Phenybutenoid monomers from the rhizomes of Zingiber cassumunar. Phytochemistry. 1996; 39(2): 459-461, article.

Han AR, Kim H, Piao D, Jung CH, Seo EK. Phytochemicals and Bioactivities of Zingiber cassumunar Roxb. Molecules. 2021; 26(8): 2377, CrossRef.

Sanatombi R, Sanatombi K. Biotechnology of Zingiber montanum (Koenig) Link ex A. Dietr.: a review. J Appl Res Med Aromat Plants. 2017; 4: 1-4, CrossRef.

DOI: https://doi.org/10.18585/inabj.v14i3.1967

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