Electric Field Stimulation of Stem Cell Culture Media Increases Oxygen Radical Uptake Capacity
Abstract
BACKGROUND: Stem cell culture medium is garnering attention because it comprises growth factors and exosomes secreted by stem cells. In this study, stem cell culture medium was subjected to electric field stimulation using the cells alive system (CAS) to determine the antiaging effects of this medium on dermal fibroblasts.
METHODS: The medium was added to the monolayer cultures of human fibroblasts with or without 100 μg/mL advanced glycation end products (AGE), and the cells were incubated for 4 hours. Total RNA was extracted, and mRNA levels of several AGE receptors and heat shock proteins were measured using quantitative reverse transcriptase-polymerase chain rection (qRT-PCR).
RESULTS: Similar amounts of exosomes were extracted from the supernatants of stem cells cultured with (CAS+) and without (CAS−) electric field stimulation. The results demonstrated that CAS+ had a higher oxygen radical absorbance capacity than CAS−, indicating its high antioxidant activity. Furthermore, the expression of domain-containing link scavenger receptor-1 mRNA, which is involved in the degradation of cell surface receptors for AGE under glycation stress, significantly increased. The electric field stimulation also increased the expression of several heat shock proteins, which play important roles in cellular responses generated against glycation stress. The electric field stimulation of the stem cell culture medium effectively promoted the endocytosis and degradation of AGE, thereby exerting antioxidant effects and inducing glycation stress.
CONCLUSION: Electric field stimulation of stem cell culture medium enhanced the expression of Hsp and its antioxidant activity in fibroblasts.
KEYWORDS: AGE, electric field stimulation, exosome, fibroblast, oxygen radical absorbance capacity, stem cell
Full Text:
PDFReferences
Meiliana A, Dewi NM, Wijaya A. Stem cell therapy in wound healing and tissue regeneration. Indones Biomed J. 2016; 8(2): 61-70, CrossRef.
Meliliana A, Wijaya A. The stem cell hypothesis of aging. Indones Biomed J. 2010; 2(1): 26-44, CrossRef.
Damayanti S, Triana R, Chouw A, Dewi NM. Is stem cell a curer or an obstruction? Mol Cell Biomed Sci. 2017; 1(1): 17-27, CrossRef.
Sandra F, Lahirin R. Human umbilical cord blood serum has higher potential in inducing proliferation of fibroblast than fetal bovine serum. Mol Cell Biomed Sci. 2017; 1(2): 65-9, CrossRef.
Liesveld JL, Sharma N, Aljitawi OS. Stem cell homing: From physiology to therapeutics. Stem Cells. 2020; 38(10): 1241-53, CrossRef.
Madrigal M, Rao KS, Riordan NH. A review of therapeutic effects of mesenchymal stem cell secretions and induction of secretory modification by different culture methods. J Transl Med. 2014; 12: 260, CrossRef.
Liang L, Wang L, Zhou S, Li J, Meng L, Zhang H, et al. Exosomes derived from human umbilical cord mesenchymal stem cells repair injured endometrial epithelial cells. J Assist Reprod Genet. 2020; 37(2): 395-403, CrossRef.
Maremanda KP, Sundar IK, Rahman I. Protective role of mesenchymal stem cells and mesenchymal stem cell-derived exosomes in cigarette smoke-induced mitochondrial dysfunction in mice. Toxicol Appl Pharmacol. 2019; 385: 114788, CrossRef.
Zomer A, Maynard C, Verweij FJ, Kamermans A, Schäfer R, Beerling E, et al. In vivo imaging reveals extracellular vesicle-mediated phenocopying of metastatic behavior. Cell. 2015; 161(5): 1046-57, CrossRef.
Hoshino A, Costa-Silva B, Shen TL, Rodrigues G, Hashimoto A, Tesic Mark M, et al. Tumour exosome integrins determine organotropic metastasis. Nature. 2015; 527(7578): 329-35, CrossRef.
Shohara R, Yamamoto A, Takikawa S, Iwase A, Hibi H, Kikkawa F, et al. Mesenchymal stromal cells of human umbilical cord Wharton’s jelly accelerate wound healing by paracrine mechanisms. Cytotherapy. 2012; 14(10): 1171-81, CrossRef.
Yagi M, Yonei Y. Glycative stress and anti-aging. Glycative Stress Res. 2018; 5: 50-4, CrossRef.
Slavin JL, Lloyd B. Health benefits of fruits and vegetables. Adv Nutr. 2012; 3(4): 506-16, CrossRef.
Cerami A, Vlassara H, Brownlee M. Glucose and aging. Sci Am. 1987; 256(5): 90-6, CrossRef.
Goldin A, Beckman JA, Schmidt AM, Creager MA. Advanced glycation end products: Sparking the development of diabetic vascular injury. Circulation. 2006; 114(6): 597-605, CrossRef.
Dyer DG, Dunn JA, Thorpe SR, Bailie KE, Lyons TJ, McCance DR, et al. Accumulation of Maillard reaction products in skin collagen in diabetes and aging. J Clin Invest. 1993; 91(6): 2463-9, CrossRef.
Reiser KM. Nonenzymatic glycation of collagen in aging and diabetes. Proc Soc Exp Biol Med. 1998; 218(1): 23-37, CrossRef.
Reddy VP, Obrenovich ME, Atwood CS, Perry G, Smith MA. Involvement of Maillard reactions in Alzheimer disease. Neurotox Res. 2002; 4(3): 191-209, CrossRef.
Schram MT, Schalkwijk CG, Bootsma AH, Fuller JH, Chaturvedi N, Stehouwer CDA, et al. Advanced glycation end products are associated with pulse pressure in type 1 diabetes: The EURODIAB prospective complications study. Hypertension. 2005; 46(1): 232-7, CrossRef.
Brownlee M, Vlassara H, Kooney A, Ulrich P, Cerami A. Aminoguanidine prevents diabetes-induced arterial wall protein cross-linking. Science. 1986; 232(4758): 1629-32, CrossRef.
Saito M, Fujii K, Soshi S, Tanaka T. Reductions in degree of mineralization and enzymatic collagen cross-links and increases in glycation-induced pentosidine in the femoral neck cortex in cases of femoral neck fracture. Osteoporos Int. 2006; 17(7): 986-95, CrossRef.
Matsuoka T, Takanashi K, Dan K, Yamamoto K, Tomobe K, Shinozuka T. Effects of mesenchymal stem cell-derived exosomes on oxidative stress responses in skin cells. Mol Biol Rep. 2021; 48(5): 4527-35, CrossRef.
Fujinami K, Dan K, Tanaka-Kagawa T, Kawamura I. Anti-aging effects of polyoxometalates on skin. Appl Sci. 2021; 11(24): 11948, CrossRef.
Maeda S, Matsui T, Ojima A, Takeuchi M, Yamagishi SI. Sulforaphane inhibits advanced glycation end product-induced pericyte damage by reducing expression of receptor for advanced glycation end products. Nutr Res. 2014; 34(9): 807-13, CrossRef.
Chen YH, Chen ZW, Li HM, Yan XF, Feng B. AGE/RAGE-induced EMP release via the NOX-derived ROS pathway. J Diabetes Res. 2018; 2018: 6823058, CrossRef.
Stoll SJ, Bartsch S, Kroll J. HOXC9 regulates formation of parachordal lymphangioplasts and the thoracic duct in zebrafish via Stabilin 2. Plos One. 2031; 8(3): e58311, CrossRef.
Draude G, Lorenz RL. TGF-beta1 downregulates CD36 and scavenger receptor A but upregulates LOX-1 in human macrophages. Am J Physiol Heart Circ Physiol. 2000; 278(4): H1042-8, CrossRef.
Yamada Y, Ishibashi K, Ishibashi K, Bhutto IA, Tian J, Lutty GA, et al. The expression of advanced glycation endproduct receptors in rpe cells associated with basal deposits in human maculas. Exp Eye Res. 2006; 82(5): 840-8, CrossRef.
Casselmann C, Reimann A, Friedrich I, Schubert A, Silber RE, Simm A. Age-dependent expression of advanced glycation end product receptor genes in the human heart. Gerontology. 2004; 50(3): 127-34, CrossRef.
Tamura Y, Adachi H, Osuga J, Ohashi K, Yahagi N, Sekiya M, et al. FEEL-1 and FEEL-2 are endocytic receptors for advanced glycation end products. J Biol Chem. 2003; 278(15): 12613-7, CrossRef.
Inoue Y, Hanazono Y, Noi K, Kawamoto A, Kimatsuka M, Harada R, et al. Split conformation of Chaetomium thermophilum Hsp104 disaggregase. Structure. 2021; 29(7): 721-30.e6, CrossRef.
Udono H, Levey DL, Srivastava PK. Cellular requirements for tumor-specific immunity elicited by heat shock proteins: Tumor rejection antigen gp96 primes CD8+ T cells in vivo. Proc Natl Acad Sci USA. 1994; 91(8): 3077-81, CrossRef.
Pozo FM, Oda T, Sekimoto T, Murakumo Y, Masutani C, Hanaoka F, et al. Molecular chaperone Hsp90 regulates REV1-mediated mutagenesis. Mol Cell Biol. 2011; 31(16): 3396-409, CrossRef.
Rapoport I, Boll W, Yu A, Böcking T, Kirchhausen T. A motif in the clathrin heavy chain required for the Hsc70/auxilin uncoating reaction. Mol Biol Cell. 2008; 19(1): 405-13, CrossRef.
Cuervo AM. Chaperone-mediated autophagy: Dice's 'wild' idea about lysosomal selectivity. Nat Rev Mol Cell Biol. 2011; 12: 535-41, CrossRef.
Horwich AL. Chaperonin-mediated protein folding. J Biol Chem. 2013; 288(33): 23622-32, CrossRef.
Barakat BM, Ahmed HI, Bahr HI, Elbahaie AM. Protective effect of boswellic acids against doxorubicin-induced hepatotoxicity: Impact on Nrf2/HO-1 defense pathway. Oxid Med Cell Longev. 2018; 2018: 8296451, CrossRef.
DOI: https://doi.org/10.18585/inabj.v15i4.2344
Copyright (c) 2023 The Prodia Education and Research Institute
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Indexed by:
The Prodia Education and Research Institute
