Effects of Hydrogen-Rich Water on Interleukin-1β, Number of Osteoclasts and Osteoblasts in Streptozotocin-induced Diabetic Rats with Orthodontic Tooth Movement

Lina Hadi, Albert Manggading Hutapea, Florenly Florenly

Abstract


BACKGROUND: Orthodontic tooth movement (OTM) may increase the risk of treatment-related complications for some diabetes mellitus (DM) patients. Hydrogen-rich water (HRW) has been demonstrated in many studies to reduce oxidative stress and cell damage. This study aimed to examine the levels of interleukin (IL)-1β, blood glucose level, body weight, tooth displacement, and population of osteoclasts and osteoblasts in diabetic rats with OTM.

METHODS: Thirty rats (Rattus novergicus) were divided into 6 groups: OTM, HRW, DM, DM+OTM, DM+HRW, and DM+OTM+HRW. DM, DM+OTM, DM+HRW, and DM+OTM+HRW groups were induced with streptozotocin (STZ) after 8 weeks of high-fat diet (HFD) and continued being fed HFD for 4 weeks. OTM, DM+OTM, and DM+OTM+HRW groups were placed on an orthodontic device for the orthodontic treatment. HRW, DM+HRW, and DM+OTM+HRW groups were administered HRW via oral gavage 3 times a day for 4 weeks. At the end of the study, all rats were euthanized and blood samples were collected for IL-1β measurement using enzyme-linked immunosorbent assay (ELISA) kits. Meanwhile, the rats’ maxilla was taken to measure tooth movement, and the number of osteoclast and osteoblast were counted.

RESULTS: The highest increase of IL-1β was in the DM+OTM group (140.07±5.14 pg/mL) and the lowest was in the HRW group (92.80±2.89 pg/mL). The average number of osteoblasts were higher in tension sites, while osteoclasts were higher in pressure sites.

CONCLUSION: Consumption of HRW in STZ-induced diabetic rats with OTM can reduce IL-1β levels, reduce tooth mobility, and promote bone remodeling.

KEYWORDS: diabetes mellitus, hydrogen rich water, interleukin-1β, orthodontic tooth movement, osteoblast, osteoclast


Full Text:

PDF

References


Alqahtani H. Medically compromised patients in orthodontic practice: Review of evidence and recommendations. Int Orthod. 2019; 17(4): 776–88, CrossRef.

Almadih A, Al-Zayer M, Dabel S, Alkhalaf A, Al Mayyad A, Bardisi W, et al. Orthodontic treatment consideration in diabetic patients. J Clin Med Res. 2018; 10(2): 77–81, CrossRef.

Maedler K, Dharmadhikari G, Schumann DM, Størling J. Interleukin-targeted therapy for metabolic syndrome and type 2 diabetes. Handb Exp Pharmacol. 2011; 203: 257–78, CrossRef.

Sukmawati IR, Donoseputro M, Lukito W. Association between free fatty acid (FFA) and insulin resistance: The role of inflammation (adiponectin and high sensivity C-reactive protein/hs-CRP) and stress oxidative (superoxide dismutase/SOD) in obese non-diabetic individual. Indones Biomed J. 2009; 1(3): 71–5, CrossRef.

Matsumoto Y. Proinflammatory mediators related to orthodontically induced periapical root resorption in rat mandibular molars. Eur J Orthod. 2017; 39(6): 686–91, CrossRef.

Di Domenico M, D’Apuzzo F, Feola A, Cito L, Monsurrò A, Pierantoni GM, et al. Cytokines and VEGF induction in orthodontic movement in animal models. J Biomed Biotechnol. 2012: 2012: 201689, CrossRef.

Graves DT, Ding Z, Yang Y. The impact of diabetes on periodontal diseases. Periodontol 2000. 2020; 82(1): 214–24, CrossRef.

Plut A, Sprogar Š, Drevenšek G, Hudoklin S, Zupan J, Marc J, et al. Bone remodeling during orthodontic tooth movement in rats with type 2 diabetes. Am J Orthod Dentofac Orthop. 2015; 148(6): 1017–25, CrossRef.

Bletsa A, Berggreen E, Brudvik P. Interleukin-1α and tumor necrosis factor-α expression during the early phases of orthodontic tooth movement in rats. Eur J Oral Sci. 2006; 114(5): 423–9, CrossRef.

Andrade I, Taddei SRA, Souza PEA. Inflammation and tooth movement: The role of cytokines, chemokines, and growth factors. Semin Orthod. 2012; 18(4): 257–69, CrossRef.

Kajiyama S, Hasegawa G, Asano M, Hosoda H, Fukui M, Nakamura N, et al. Supplementation of hydrogen-rich water improves lipid and glucose metabolism in patients with type 2 diabetes or impaired glucose tolerance. Nutr Res. 2008; 28(3): 137–43, CrossRef.

Kamimura N. Molecular hydrogen improves obesity and diabetes by inducing hepatic FGF21 and stimulating energy metabolism in db/db mice. Obesity. 2011; 19(7): 1396–403, CrossRef.

Yoneda T, Tomofuji T, Kunitomo M, Ekuni D, Irie K, Azuma T, et al. Preventive effects of drinking hydrogen-rich water on gingival oxidative stress and alveolar bone resorption in rats fed a high-fat diet. Nutrients. 2017; 9(1): 64, CrossRef.

Tomofuji T, Kawabata Y, Kasuyama K, Endo Y, Yoneda T, Yamane M, et al. Effects of hydrogen-rich water on aging periodontal tissues in rats. Sci Rep. 2014; 4: 5534, CrossRef.

Kasuyama K, Tomofuji T, Ekuni D, Tamaki N, Azuma T, Irie K, et al. Hydrogen-rich water attenuates experimental periodontitis in a rat model. J Clin Periodontol. 2011; 38(12): 1085–90, CrossRef.

Zhou X, Zhang W, Liu X, Zhang W, Li Y. Interrelationship between diabetes and periodontitis: Role of hyperlipidemia. Arch Oral Biol. 2015; 60(4): 667–74, CrossRef.

King AJ. The use of animal models in diabetes research. Br J Pharmacol. 2012; 166(3): 877–94, CrossRef.

Skovsø S. Modeling type 2 diabetes in rats using high fat diet and streptozotocin. J Diabetes Investig. 2014; 5(4): 349–58, CrossRef.

Yuliana. Penurunan kadar glukosa darah dan hitung sel kupffer tikus hiperglikemik setelah pemberian dekok daun salam. J Vet. 2014; 15(4): 541–7, article.

Ismawati, Asni E, Mukhyarjon, Romus I. Alpha lipoic acid inhibits expression of intercellular adhesion molecule-1 (ICAM-1) in type 2 diabetic mellitus rat models. Indones Biomed J. 2020; 12(1): 40–4, CrossRef.

Parasuraman S, Raveendran R, Kesavan R. Blood sample collection in small laboratory animals. J Pharmacol Pharmacother. 2010; 1(2): 87–93, CrossRef.

Alhashimi N, Frrithiof L, Brudvik P, Bakhiet M. Orthodontic tooth movement and de novo synthesis of proinflammatory cytokines. Am J Orthod Dentofac Orthop. 2001; 119(3): 307–12, CrossRef.

Ferreira CL, da Rocha VC, da Silva Ursi WJ, De Marco AC, Santamaria M Jr, Santamaria MP, et al. Periodontal response to orthodontic tooth movement in diabetes-induced rats with or without periodontal disease. J Periodontol. 2018; 89(3): 341–50, CrossRef.

Greenfield EA. Sampling and preparation of mouse and rat serum. Cold Spring Harb Protoc. 2017; 2017(11): pdb.prot100271, CrossRef.

Santamaria-Jr M, Bagne L, Zaniboni E, Santamaria MP, Jardini MAN, Felonato M, et al. Diabetes mellitus and periodontitis: Inflammatory response in orthodontic tooth movement. Orthod Craniofacial Res. 2020; 23(1): 27–34, CrossRef.

Yan D, Kang P, Shen B, Yang J, Zhou Z, Duan L, et al. Serum levels of IL-1β, IL-6 and TNF-α in rats fed with Kashin-Beck disease-affected diet. Int J Rheum Dis. 2010; 13(4): 406–11, CrossRef.

Liu Y, Wang DL, Huang YC, Wang TB, Zeng H. Hydrogen inhibits the osteoclastogenesis of mouse bone marrow mononuclear cells. Mater Sci Eng C Mater Biol Appl. 2020: 110: 110640, CrossRef.

Saputro A, Prasetyo SA, Sobirin MA, Mughni A, Prajoko YW. Sleeve gastrectomy decrease body weight, fasting blood glucose, and gene expression of TNF-α and IL-1 in the abdominal aorta of rats with obesity and diabetes mellitus. Indones Biomed J. 2023; 15(4): 316–21, CrossRef.

Bai Y, Wang C, Jiang H, Wang L, Li N, Zhang W, et al. Effects of hydrogen rich water and pure water on periodontal inflammatory factor level, oxidative stress level and oral flora: A systematic review and meta-analysis. Ann Transl Med. 2022; 10(20): 1120, CrossRef.

Kajisa T, Yamaguchi T, Hu A, Suetake N, Kobayashi H. Hydrogen water ameliorates the severity of atopic dermatitis-like lesions and decreases interleukin-1β, interleukin-33, and mast cell infiltration in NC/Nga mice. Saudi Med J. 2017; 38(9): 928–33, CrossRef.

Braga SMG, De Albuquerque Taddei SR, Andrade I, Queiroz-Junior CM, Garlet GP, Repeke CE, et al. Effect of diabetes on orthodontic tooth movement in a mouse model. Eur J Oral Sci. 2011; 119(1): 7–14, CrossRef.

Yang L, Li D, Chen S. Hydrogen water reduces NSE, IL-6, and TNF-α levels in hypoxic-ischemic encephalopathy. Open Med. 2016; 11(1): 399–406, CrossRef.

Asada R, Tazawa K, Sato S, Miwa N. Effects of hydrogen-rich water prepared by alternating-current-electrolysis on antioxidant activity, DNA oxidative injuries, and diabetes-related markers. Med Gas Res. 2020; 10(3): 114–21, CrossRef.

Ahmad MF, Haidar MA, Naseem N, Ahsan H, Siddiqui WA. Hypoglycaemic, hypolipidaemic and antioxidant properties of Celastrus paniculatus seed extract in STZ-induced diabetic rats. Mol Cell Biomed Sci. 2023; 7(1): 10–7, CrossRef.

Li Y, Jacox LA, Little SH, Ko C. Orthodontic tooth movement: The biology and clinical implications. Kaohsiung J Med Sci. 2018; 34(4): 207–14, CrossRef.




DOI: https://doi.org/10.18585/inabj.v15i6.2677

Copyright (c) 2023 The Prodia Education and Research Institute

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

 

Indexed by:

                  

               

                   

 

 

The Prodia Education and Research Institute