The Protective Effect of Andrographis paniculata Against Lipopolysaccharide-induced Sepsis in Lung Tissues of a Rat Model Through the Decrease of ICAM-1 and E-selectin Expression

Michael Kevin, Nyoman Suci Widyastiti, Selamat Budijitno, Yan Wisnu Prajoko, Neni Susilaningsih

Abstract


BACKGROUND: Andrographis paniculata has anti-inflammatory, anti-cancer, and immunomodulatory effects. Intercellular adhesion molecule-1 (ICAM-1) and E-selectin are released in inflammatory conditions due to sepsis. In sepsis-associated fatalities, E-selectin is strongly expressed on pulmonary microvasculature and ICAM-1 is strongly expressed on pulmonary microvasculature endothelial cells, pulmonary macrophages, and lymphocytes. Therefore, this study was conducted to analyze the protective effect of A. paniculata leaf extract against lung injury in lipopolysaccharide (LPS)-induced sepsis mouse model.

METHODS: Thirty male Wistar rats were divided into 1 control group and 4 sepsis groups. The control group was fed ad libitum for 14 days, followed by an intraperitoneal injection of 5 mg/kgBW 0.9% NaCl on day-21. While the sepsis groups were fed ad libitum and orally administered 0, 200, 400, and 500 mg/kgBW of A. paniculata leaf extract, respectively, for 14 days. All four sepsis groups were intraperitoneally injected with 5 mg/kgBW LPS on day-21. Mice termination and measurement of ICAM-1 and E-selectin were performed on day-25. The immunohistochemical examination of ICAM-1 expression was performed using ICAM-1/CD54 antibody, meanwhile E-selectin expression was examined using primary antibody E-Selectin (D-7) rabbit IgG antibody.

RESULTS: All groups given A. paniculata leaf extract showed lower expression of ICAM-1 (p<0.001) and E-selectin (p<0.001) than the group without extract. A. paniculata leaf extract 500 mg/kgBW gives the best effectiveness compared to other doses.

CONCLUSION: A. paniculata leaf extract has a protective effect against lung injury by lowering the expression of ICAM-1 and E-selectin in lung tissue.

KEYWORDS: ICAM-1, E-selectin, sepsis, Andrographis paniculata, andrographolide


Full Text:

PDF

References


Cecconi M, Evans L, Levy M, Rhodes A. Sepsis and septic shock. Lancet. 2018; 392(10141): 75-87, CrossRef.

Evans L, Rhodes A, Alhazzani W, Antonelli M, Coopersmith CM, French C, et al. Surviving sepsis campaign: International guidelines for management of sepsis and septic shock 2021. Intensive Care Med. 2021; 47(11): 1181-247, CrossRef.

Gyawali B, Ramakrishna K, Dhamoon AS. Sepsis: The evolution in definition, pathophysiology, and management. SAGE Open Med. 2019; 7: 2050312119835043, CrossRef.

Ou H, Xiao X, Jiang Y, Peng Y, Yang M, Gao M. Expression of microRNA-23b in patients with sepsis and its effect on leukocytes and the expression of E-selectin and ICAM-1. Exp Ther Med. 2018; 16(6): 4707-11, CrossRef.

Ode Y, Aziz M, Wang P. CIRP increases ICAM-1+ phenotype of neutrophils exhibiting elevated iNOS and NETs in sepsis. J Leukoc Biol. 2018; 103(4): 693-707, CrossRef.

Silva M, Videira PA, Sackstein R. E-selectin ligands in the human mononuclear phagocyte system: Implications for infection, inflammation, and immunotherapy. Front Immunol. 2018: 8: 1878, CrossRef.

Bui TM, Wiesolek HL, Sumagin R. ICAM-1: A master regulator of cellular responses in inflammation, injury resolution, and tumorigenesis. J Leukoc Biol. 2020; 108(3): 787-99, CrossRef.

Lin HC, Li CC, Yang YC, Chiu TH, Liu KL, Lii CK, et al. Andrographis paniculata diterpenoids and ethanolic extract inhibit TNFα-induced ICAM-1 expression in EA.hy926 cells. Phytomedicine. 2019; 52: 157-67, CrossRef.

Lu CY, Yang YC, Li CC, Liu KL, Lii CK, Chen HW. Andrographolide inhibits TNFα-induced ICAM-1 expression via suppression of NADPH oxidase activation and induction of HO-1 and GCLM expression through the PI3K/Akt/Nrf2 and PI3K/Akt/AP-1 pathways in human endothelial cells. Biochem Pharmacol. 2014; 91(1): 40-50, CrossRef.

Hossain MS, Urbi Z, Sule A, Hafizur Rahman KM. Andrographis paniculata (Burm. f.) Wall. ex Nees: A review of ethnobotany, phytochemistry, and pharmacology. ScientificWorldJournal. 2014; 2014: 274905, CrossRef.

Islam MT, Ali ES, Uddin SJ, Islam MA, Shaw S, Khan IN, et al. Andrographolide, a diterpene lactone from Andrographis paniculata and its therapeutic promises in cancer. Cancer Lett. 2018; 420: 129-45, CrossRef.

Yao H, Zhao J, Zhu L, Xie Y, Zhao N, Yao R, et al. Protective effect of the effective part of Andrographis paniculata (Burm.f.) Nees on PM 2.5-induced lung injury in rats by modulating the NF-κB pathway. J Ethnopharmacol. 2021: 280: 114420, CrossRef.

Gou T, Hu M, Xu M, Chen Y, Chen R, Zhou T, et al. Novel wine in an old bottle: Preventive and therapeutic potentials of andrographolide in atherosclerotic cardiovascular diseases. J Pharm Anal. 2023; 13(6): 563-89, CrossRef.

La Russa R, Maiese A, Viola RV, De Matteis A, Pinchi E, Frati P, et al. Searching for highly sensitive and specific biomarkers for sepsis: State-of-the-art in post-mortem diagnosis of sepsis through immunohistochemical analysis. Int J Immunopathol Pharmacol. 2019; 33: 2058738419855226, CrossRef.

Muz B, Abdelghafer A, Markovic M, Yavner J, Melam A, Salama NN, et al. Targeting E-selectin to tackle cancer using uproleselan. Cancers. 2021; 13(2): 335, CrossRef.

Birnhuber A, Fließer E, Gorkiewicz G, Zacharias M, Seeliger B, David S, et al. Between inflammation and thrombosis: Endothelial cells in COVID-19. Eur Respir J. 2021; 58(3): 2100377, CrossRef.

Theofilis P, Sagris M, Oikonomou E, Antonopoulos AS, Siasos G, Tsioufis C, et al. Inflammatory mechanisms contributing to endothelial dysfunction. Biomedicines. 2021; 9(7): 781, CrossRef.

Nakano H, Inoue S, Shibata Y, Abe K, Murano H, Yang S, et al. E-selectin as a prognostic factor of patients hospitalized due to acute inflammatory respiratory diseases: A single institutional study. EXCLI J. 2019; 18: 1062-70, CrossRef.

Blondonnet R, Constantin JM, Sapin V, Jabaudon M. A pathophysiologic approach to biomarkers in acute respiratory distress syndrome. Dis Markers. 2016: 2016: 3501373, CrossRef.

Lim JC, Goh FY, Sagineedu SR, Yong AC, Sidik SM, Lajis NH, et al. A semisynthetic diterpenoid lactone inhibits NF-κB signalling to ameliorate inflammation and airway hyperresponsiveness in a mouse asthma model. Toxicol Appl Pharmacol. 2016; 302: 10-22, CrossRef.

Prasanti D. Health information of literation as prevention processes of hoax information in the use of traditional medicine in digital era. J Pekommas. 2018; 3(1): 45-52, CrossRef.

Sikumalay A, Suharti N, Masri M. Efek antibakteri dari rebusan daun sambiloto (Andrographis paniculata Nees) dan produk herbal sambiloto terhadap Staphylococcus aureus. J Kesehat Andalas. 2014; 5(1): 196-200, CrossRef.

Kumar S, Singh B, Bajpai V. Andrographis paniculata (Burm.f.) Nees: Traditional uses, phytochemistry, pharmacological properties and quality control/quality assurance. J Ethnopharmacol. 2021: 275: 114054, CrossRef.

Peng S, Hang N, Liu W, Guo W, Jiang C, Yang X, et al. Andrographolide sulfonate ameliorates lipopolysaccharide-induced acute lung injury in mice by down-regulating MAPK and NF-κB pathways. Acta Pharm Sin B. 2016; 6(3): 205-11, CrossRef.

Dai Y, Chen SR, Chai L, Zhao J, Wang Y, Wang Y. Overview of pharmacological activities of Andrographis paniculata and its major compound andrographolide. Crit Rev Food Sci Nutr. 2019; 59(Suppl1): S17-29, CrossRef.

Prananta ABB, Widyastiti NS, Ariosta A, Retnoningrum D, Salsabela R, Karlowee V, et al. Renoprotective effect of sambiloto (Andrographis paniculata) leaf extract on lipopolysaccharide-induced septic rats. J Biomed Transl Res. 2023; 9(1): 25-30, CrossRef.

Salsabela R, Widyastiti NS, Retnoningrum D, Ariosta A, Susilaningsih N. Effects of Andrographis paniculata leaf extract on C-reactive protein and serum ferritin in lipopolysaccharide-induced sepsis model rat. J Kedokt Diponegoro (Diponegoro Med J). 2023; 12(3): 131-6, CrossRef.

Zhu ZT, Jiang XS, Wang BC, Meng WX, Liu HY, Tian Y. Andrographolide inhibits intimal hyperplasia in a rat model of autogenous vein grafts. Cell Biochem Biophys. 2011; 60(3): 231-39, CrossRef.

Wang YJ, Wang JT, Fan QX, Geng JG. Andrographolide inhibits NF-κB activation and attenuates neointimal hyperplasia in arterial restenosis. Cell Res. 2007; 17(11): 933-41, CrossRef.

Bodiga VL, Bathula J, Kudle MR, Vemuri PK, Bodiga S. Andrographolide suppresses cisplatin-induced endothelial hyperpermeability through activation of PI3K/Akt and eNOS-derived nitric oxide. Bioorganic Med Chem. 2020; 28(23): 115809, CrossRef.

Vassiliou AG, Kotanidou A, Dimopoulou I, Orfanos SE. Endothelial damage in acute respiratory distress syndrome. Int J Mol Sci. 2020; 21(22): 8793, CrossRef.

Lv Y, Kim K, Sheng Y, Cho J, Qian Z, Zhao YY, et al. YAP controls endothelial activation and vascular inflammation through TRAF6. Circ Res. 2018; 123(1): 43-56, CrossRef.

Veldman LBM, Belt-Van Zoen E, Baars EW. Mechanistic evidence of Andrographis paniculata (Burm. f.) wall. ex Nees, Pelargonium sidoides DC., Echinacea species and a combination of Hedera helix L., Primula veris L./Primula elatior L. and Thymus vulgaris L./Thymus zygis L. in the treatment of acute, uncomplicated respiratory tract infections: A systematic literature review and expert interviews. Pharmaceuticals. 2023; 16(9): 1206, CrossRef.

Joselin J, Jeeva S. Andrographis paniculata: A review of its traditional uses, phytochemistry and pharmacology. Med Aromat Plants. 2014; 3(4): 1000169, CrossRef.

Zhong L, Simard MJ, Huot J. Endothelial microRNAs regulating the NF-κB pathway and cell adhesion molecules during inflammation. FASEB J. 2018; 32(8): 4070-84, CrossRef.

Dayang EZ, Plantinga J, Ter Ellen B, van Meurs M, Molema G, Moser J. Identification of LPS-activated endothelial subpopulations with distinct inflammatory phenotypes and regulatory signaling mechanisms. Front Immunol. 2019: 10: 1169, CrossRef.

Shapiro NI, Schuetz P, Yano K, Sorasaki M, Parikh SM, Jones AE, et al. The association of endothelial cell signaling, severity of illness, and organ dysfunction in sepsis. Crit Care. 2010; 14(5): R182, CrossRef.

Zonneveld R, Martinelli R, Shapiro NI, Kuijpers TW, Plötz FB, Carman CV. Soluble adhesion molecules as markers for sepsis and the potential pathophysiological discrepancy in neonates, children and adults. Crit Care. 2014; 18(1): 204, CrossRef.

Muliaty D, Yusuf I, Hermawan AG. Lipopolysaccharide binding protein, soluble-intercellular adhesion molecule-1, procalcitonin, and protein C activity and clinical outcome in systemic inflammatory response syndrome (SIRS) or sepsis patients. Indones Biomed J. 2009; 1(1): 73-9, CrossRef.

Dzikowska-Diduch O, Domienik-Karłowicz J, Górska E, Demkow U, Pruszczyk P, Kostrubiec M. E-selectin and sICAM-1, biomarkers of endothelial function, predict recurrence of venous thromboembolism. Thromb Res. 2017; 157: 173-80, CrossRef.




DOI: https://doi.org/10.18585/inabj.v15i6.2693

Copyright (c) 2023 The Prodia Education and Research Institute

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

 

Indexed by:

                  

               

                   

 

 

The Prodia Education and Research Institute