The Effect of Polysaccharides Peptides Ganoderma Lucidum to Aortic Foam Cell Count and Lipid Profile in Type 2 Diabetic Model Rattus Norvegicus Strain Wistar

Djanggan Sargowo, Titin Andri Wihastuti, Cathrine Theodora Sukotjo, Prasanti Mahesa Anjani, Olivia Handayani, Liemena Harold Adrian


BACKGROUND: Type 2 Diabetes Mellitus (DM) is one of dominant factors in cardiovascular deaths and induces endothelial dysfunction. A definite sign of endothelial dysfunction is foam cells formation derived from oxidized LDL. This study was conducted to determine effect of Polysaccharides Peptides (PsP) Ganoderma lucidum to foam cell counts and lipid profile in diabetic model rats with high-fat diet.

METHODS: Treatment group was categorized to negative control, positive control, DM-50, DM-150, and DM-300. Type 2 DM induced by Streptozotocin. PsP is administered with predetermined doses on each treatment’s group (50, 150, and 300 mg/kgBW) for 12 weeks. Measurements of foam cell count were held after obtaining rat’s aorta. To assess lipid profile, blood sample was taken from the rat's heart. Data analyzed by One-way ANOVA and Post Hoc Tukey tests.

RESULTS: The administration of Ganoderma lucidum PsP to diabetic model rats provided a significant difference in lowering foam cell (p=0.017; CI 95%). It also gave significant difference between levels of each lipid components (Total Cholesterol, Trygliceride, LDL and HDL) in at least two treatment groups (p=0.010; CI 95%). Based on Post Hoc Tukey tests, the relationship between administration of PsP and foam cell is significant (p=0.002).

CONCLUSION: PsP has antioxidant and anti-inflammatory effect in type 2 DM model Rattus norvegicus strain wistar, which can inhibit atherosclerosis and endothelial damage process. Further studies are necessary to evaluate the benefits of PsP as an adjuvant therapy in management of diabetic dyslipidemia.

KEYWORDS: PsP, Ganoderma lucidum, foam cell, lipid profile, DM

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International Diabetes Federation. IDF Diabetes Atlas 6th edition Year: 2013. Brussels: International Diabetes Federation; 2013, article.

Hamamdzic D, Wilensky RL. Porcine models of accelerated coronary atherosclerosis: role of diabetes mellitus and hypercholesterolemia. J Diabetes Res. 2013; 2013: 761415, CrossRef.

WHO [Internet]. Cardiovascular Diseases 2011 (cited 2013 Nov 15). Avaliable from:

NHLBI [Internet]. Bethesda: National Institutes of Health, Department of Health and Human Services. What Is Atherosclerosis? (cited 2013 Nov 15). Avaliable from:

Kushiyama A, Okubo H, Sakoda H, Kikuchi T, Fujishiro M, Sato H, et al. Xanthine oxidoreductase is involved in macrophage foam cell formation and atherosclerosis development. Arterioscler Thromb Vasc Biol. 2012; 32: 291-8, CrossRef.

Mooradian AD. Dyslipidemia in type 2 diabetes mellitus. Nat Clin Pract Endocrinol Metab. 2009; 5: 150-9, CrossRef.

Vijayaraghavan K. Treatment of dyslipidemia in patients with type 2 diabetes. Lipids Health Dis. 2010; 9: 144, CrossRef.

National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III). Third Report of the National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III) final report. Circulation. 2002; 106: 3142-421, PMID.

Scott JA, King GL. Oxidative stress and antioxidant treatment in diabetes. Ann NY Acad Sci. 2004; 1031: 204-13, CrossRef.

Jia J, Zhang X, Hu YS, Wu Y, Wang QZ, Li NN, et al. Evaluation of in vivo antioxidant activities of Ganoderma lucidum polysaccharides in STZ-diabetic rats. Food Chem. 2008; 115: 32-6, CrossRef.

Li F, Zhang Y, Zhong Z. Antihyperglycemic effect of ganoderma lucidum polysaccharides on streptozotocin-induced diabetic mice. Int J Mol Sci. 2011; 12: 6135-45, CrossRef.

Seto SW, Lam TY, Tam HL, Au AL, Chan SW, Wu JH, et al. Novel hypoglycemic effects of Ganoderma lucidum water-extract in obese/diabetic (+db/+db) mice. Phytomedicine. 2009; 16: 426-36, CrossRef.

Venegas-Pino DE, Banko N, Khan MI, Shi Y, Werstuck GH. Quantitative analysis and characterization of atherosclerotic lesions in the murine aortic sinus. J Vis Exp. 2013; 82: 50933, CrossRef.

Mendizábal Y, Llorens S, Nava E. Hypertension in metabolic syndrome: vascular pathophysiology. Int J Hypertens. 2013; 2013: 230868, CrossRef.

Repetto M, Semprine J, Boveris A. Lipid peroxidation: Chemical mechanism, biological implications and analytical determination. In: Catala A, editor. Lipid Peroxidation. Argentina: University of Buenos Aires, School of Pharmacy and Biochemistry, General and Inorganic Chemistry, Institute of Biochemistry and Molecular Medicine (IBIMOL-UBA-CONICET); 2012. p.7-17,

Allahverdian S, Pannu PS, Francis GA. Contribution of monocyte-derived macrophages and smooth muscle cells to arterial foam cell formation. Cardiovasc Res. 2012; 95: 165-72, CrossRef.

Song YS, Kim SH, Sa JH, Jin C, Lim CJ, Park EH. Anti-angiogenic and inhibitory activity on inducible nitric oxide production of the mushroom Ganoderma lucidum. J Ethnopharmacol. 2004; 90: 17-20, CrossRef.

Chen XP, Chen Y, Li SB, Chen YG, Lan JY, Liu LP. Free radical scavenging of Ganoderma lucidum polysaccharides and its effect on antioxidant enzymes and immunity activities in cervical carcinoma rats. Carbohydr Polym. 2009; 77: 389-93, CrossRef.

Meng GL, Zhu HY, Yang SJ, Wu F, Zheng HH, Chen E, et al. Attenuating effects of Ganoderma lucidum polysaccharides on myocardial collagen cross-linking relates to advanced glycation end product and antioxidant enzymes in high-fat-diet and streptozotocin-induced diabetic rats. Carbohydr Polym. 2011; 84: 180-85, CrossRef.

Fikriah I. Effect of curcumin on the effect on the levels of total cholesterol, LDL-cholesterol, the amount of F2-Isoprostan and foam cell in aortic walls of rats with atherogenic diet. Folia Medica Indonesiana. 2007; 43: 136-40, article.

Sliva D. Ganoderma lucidum (Reishi) in cancer treatment. Integr Cancer Ther. 2003; 2: 358-64, CrossRef.


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