The Role of Ceftazidime as a Photosensitizer in Human Erythrocytes Through Oxidative Stress Mechanism

Mashuri Mashuri, Mustofa Ruhullah, Bayu Diertama Putera, Vicky Pramudinta Mega, Fadillah Alma Putra, Eko Suhartono


BACKGROUND: Ceftazidime was known to cause photosensitization reactions. However, how it plays the role remained unclear. In our study, we aim to investigate the photosensitization effect of ceftazidime in erythrocytes via oxidative stress.

METHODS: Samples were divided into six different groups: negative control group, positive control group and four experimental groups with 10%, 20%, 30%, and 40% ceftazidime, respectively. The positive control and experimental groups were exposed to ultraviolet (UV)-light for 2 hours. Superoxide radical, malondialdehyde (MDA), carbonyl compounds (CC) and methemoglobin (Met-Hb) levels were then measured.

RESULTS: The results showed a significant increased of superoxide radical, MDA, CC and Met-Hb levels in all experimental groups compared to both negative or positive control groups (p< 0.05).

CONCLUSION: In conclusion, our study confirmed the role of ceftazidime as a photosensitizer in erythrocytes via the oxidative stress mechanisms.

KEYWORDS: ceftazidime, oxidative stress, photosensitizer, photosensitization.

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Riske KA, Sudbrack TP, Archilla NL, Uchoa AF, Schroder AP, Marques CM, et al. Giant vesicles under oxidative stress induced by a membrane-anchored photosensitizer. Biophys J. 2009; 97: 1362-70, CrossRef.

Pei D, Luan J. Development of visible light-responsive sensitized photocatalysts. Int J Photoenergy. 2012; 2012: 1-13, CrossRef.

Hiraku Y, Ito K, Hirakawa K, Kawanishi S. Photosensitized DNA damage and its protection via a novel mechanism. Photochem Photobiol. 2007; 83: 205-12, CrossRef.

Quintero B, Miranda MA. Mechanisms of photosensitization induced by drugs: a general survey. Ars Pharmaceutica. 2000; 41: 27-46, article.

Dubakiene R, Kupriene M. Scientific problems of photosensitivity. Medicina. 2006; 42: 619-24, PMID.

Zuba EB, Koronowska S, Osmola-Mankowska A, Jenerowicz D. Drug-induced photosensitivity. Acta Dermatovenerol Croat. 2016; 24: 55-64, PMID.

Gill L, Lim HW. Drug-induced photosensitivity. In: Hall JC, Hall BJ, editor. Cutaneous Drug Eruptions: Diagnosis, Histopathology and Therapy. London: Springer-Verlag London; 2015. p107-21, CrossRef.

Moreno AH, Salgado HRN. Stability study and degradation kinetics of ceftazidime in pharmaceutical preparations. Adv Anal Chem. 2012; 2: 1-5, CrossRef.

Rule R, Villagra S, Barrena P, Lacchini R, Reynaldi FJ. Pharmacokinetics of ceftazidime administered to lactating and nonlactating goats. Tydskr S Afr Vet Ver. 2011; 82: 219-23, CrossRef.

Barber KE, Ortwine JK, Akins RL. Ceftazidime/avibactam: who says you can’t teach an old drug new tricks?. J Pharm Pharm Sci. 2016; 19: 448-64, CrossRef.

Suhartono E, Iskandar, Hamidah S, Arifin YF. Phytochemical constituents analysis and neuroprotective effect of leaves of gemor (nothaphoebe coriacea) on cadmium-induced neurotoxicity in rats: an in-vitro study. Int J Toxicol Pharmacol Res. 2015; 7: 297-302, article.

Suhartono E, Triawanti, Yunanto A, Firdaus RT, Iskandar. Chronic cadmium hepatooxidative in rats : treatment with haruan fish (Channa striata) extract. Approaches. In: International Conference on Environmental Science and Development, January 19-20, 2013, Dubai, United Arab Emirates. Hongkong: Asia-Pacific Chemical, Biological & Environmental Engineering Society; 2013.

Yunanto A, Iskandar, Suhartono E. In-vitro effects of some antibiotic drugs on saliva thiocyanate and oxidation protein products levels on newborn at risk of sepsis. IJPCR. 2016; 8: 86-9, article.

Suhartono E, Bahriansyah M, Triawanti. The inhibition effect of kelakai (Stenochlaena palustris) extract on cadmium-induced glycation and fructation in-vitro. IJPCR. 2016; 8: 248-53, article.

Srivastava S, Alhomida AS, Siddiq NJ, Puri SK, Pandey VC. Methemoglobin reductase activity and in vitro sensitivity towards oxidant induced methemoglobinemia in Swiss mice and beagle dogs erythrocytes. Mol Cell Biochem. 2002; 232: 81-5.

Zama I, Yakubu A, Okwesili AN, Ishaku EY, Erhabor O, Mainasara AS, et al. Prevalence of malaria parasitaemia and methaemoglobin levels among blood donors in Sokoto, Nigeria. Internal Medicine Inside. 2013; 1-4, CrossRef.

Zaheer MR, Gupta A, Iqbal J, Zia Q, Ahmad A, Roohi, et al. Molecular mechanisms of drug photodegradation and photosensitization. Current Pharm Design. 2016; 22: 1-15.

Hermann PB, Pianovski MA, Henneberg R, Nascimento AJ, Leonart MS. Erythrocyte oxidative stress markers in children with sickle cell disease. J Pediatr. 2016; 92: 394-9, CrossRef.

Kanias T, Acker JP. Bioprservation of red blood cells – the struggle with hemoglobin oxidation. FEBS Journal. 2010; 277: 343-56, CrossRef.


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