UV-Visible Spectrophotometric as a Prospective Tool in Neonatal Sepsis

Eko Suhartono, Ari Yunanto, Edi Hartoyo, Nia Kania, Adelia Anggraini Utama, Ratih Kumala Sari, Niarsari Anugrahing Putri, Iskandar Thalib


BACKGROUND: This study was aimed to employ the UV-Visible (UV-Vis) spectrophotometry techniques to detect the changes in the blood of neonatal sepsis (NS) subject for a deeper understanding in the pathomechanism of NS.

METHODS: The cross-sectional study was conducted from February to May 2017 in the Neonatology Division, Department of Pediatric, Ulin General Hospital/Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, South Kalimantan, Indonesia. Blood specimens were taken from newborns, of which 15 each of newborns at risk of sepsis and without risk of sepsis. Data were analyzed by using Mann-Whitney U test.

RESULTS: The result of this study suggested that there is a significant difference in the average of absorbance ratio parameter using UV-Vis spectrophotometric methods on the case group compared to the control group. Also, there is a significant difference between advanced oxidation protein products (AOPPs) and thiocyanate (SCN) level in newborn at risk of sepsis.

CONCLUSION: The present study demonstrated there were significant differences between the average of absorbance ratio parameter for protein and oxy-hemoglobin region using UV-Vis spectrophotometric methods in healthy subjects and newborn at risk of sepsis.

KEYWORDS: spectrophotometric, neonatal sepsis, oxidative stress

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Bokun LV, Huang J, Yuan H, Yan W, Hu G, Wang J. Tumor necrosis factor-alpha as a diagnostic marker for neonatal sepsis: A meta-analysis. Scientific World J. 2014; 2014: 1-14, CrossRef.

Yunanto A, Firdaus RT, Triawanti, Suhartono E. Advance oxidation protein products (AOPPs) of newborn at risk of sepsis as novel parameter for early-onset neonatal sepsis. Int J Biosci Biochem Bioinforma. 2014; 4: 90-3, CrossRef.

Simarmata YBC, Harahap U, Djipta GD. Identification of risk factors caused neonatal sepsis of premature neonatus in Adam Malik General Hospital Center. Int J PharmTech. 2016; 9: 54-7, article.

World Health Organization [Internet]. Global Health Observatory Data: Infant Mortality [update 2017; cited 2017 Jun 13]. Available from: http://www.who.int/.

World Health Organization [Internet]. Global Health Observatory Data: Causes of Child Mortality [cited 2017 Jun 13]. Available from: http://www.who.int/.

World Health Organization [Internet]. Global Health Observatory Data: Neonatal Mortality. [cited 2017 Jun 13]. Available from: http://www.who.int/.

Venkatesh M, Flores A, Luna RA. Molecular microbiological methods in the diagnosis of neonatal sepsis. Expert Rev Anti Infect Ther. 2010; 9: 1037-48, CrossRef.

Fink MP. Cytopathic hypoxia: Mitochondrial dysfunction as mechanism contributing to organ dysfunction in sepsis. Crit Care Clin. 2001; 1: 219-37, PMID.

Cancelier AC, Petronilho F, Reinke A, Constantino L, Machado R, Ritter C, Dal-Pizzol F. Inflammatory and oxidative parameters in cord blood as diagnostic of early-onset neonatal sepsis: A case control study. Pediatr Crit Care Med. 2009; 10: 467-71, CrossRef.

Yunanto A, Iskandar, Suhartono E. In-vitro effects of some antibiotic drugs on saliva thiocyanate and oxidation protein products levels on newborn at risk of sepsis. IJPCR. 2016; 8: 86-9, article.

Ibrahim MSM, Bajaj MM, Singh VR, Ruchika, Farhana SAS. Spectroscopic study of blood samples using FTIR and UV-Visible spectrometers. Internat J Bioscience. 2014; 3: 13-6.

Schmid F. Biological Macromolecules: UV-visible Spectrophotometry. Encyclopedia of Life Sciences. 2001; 1-4, CrossRef.

Gunasekaran S, Natarajan RK, Renganayaki V, Rathikha R. FTIR and UV visible spectrophotometric approach to discriminate leukemic sera. Asian J. Spectro. 2008; 20: 2521-30.

Kanagathara N, Thirunavukkarasu M, Jeyanthi EC, Shenbagarajan P. FTIR and UV-Visible spectral study on normal blood samples. Int. J Pharm Bio Sci. 2011; 1: 74-81, article.

Gunasekaran S, Nataraian RK, Renganayaki V. UV Visible Spectrophotometric Approach and Absorption Model for the Discrimination of Diseased Blood. Asian J Chem. 2008; 20: 48-54, CrossRef.

Gunasekaran S, Uthra D. FTIR and UV-Visible spectral study on normal and jaundice blood samples. Asian J.Chem. 2008; 20: 5695-703, article.

Bryce J, Boshi-Pinto C, Shibuya K, Black RE. WHO estimates the causes of death in children. Lancet. 2005; 365: 1147-52, CrossRef.

Aggarwal R, Sarkar N, Deorari AK, Paul VK. Sepsis in the newborn. Indian J Pediatr. 2008; 75: 261-6, CrossRef.

Gao J, Ding XS, Zhang YM, Dai DZ, Liu M, Zhang C, Dai Y. Hypoxia/oxidative stress alters the pharmacokinetics of CPU86017-RS mitochondrial dysfunction and NADPH oxidase activation. Acta Pharm Sinica. 2013; 34: 1575-84, CrossRef.

Lestarisa T, Alexandra FD, Jelita H, Suhartono E. Myeloperoxidase as an indicator of liver cells inflammation induced by mercury. IJPCR. 2016; 8: 1516-21, article.

Lane AE, Tan JTM, Hawkins CL, Heather AK, Davies MJ. The myeloperoxidase-derived oxidant HOSCN inhibits protein tyrosine phosphatases and modulates cell signalling via the mitogenactivated protein kinase (MAPK) pathway in macrophages. Biochem J. 2010; 430: 161-9, CrossRef.

Suhartono E, Nijka JA, Anhar VY, Sari RA, Edyson, Marisa D. Antilipid peroxidation activities of three selected fruits juices against cadmium induced liver damage in vitro. J. Trop Life Science. 2015; 5: 77-9, CrossRef.

Aflanie I, Sari NN, Suhartono E. Oxidative and chlorinative stress biomarkers in liver cells of rats exposed to cyanide in-vitro. IJPCR. 2016; 8: 1441-5.

DOI: https://doi.org/10.18585/inabj.v10i1.360

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