Analysis of ALDH1A1 and ALDH1A3 Gene mRNA Expressions in Adipose-Derived Stem Cells (ASCs) and Umbilical Cord Stem Cells (UCSCs)

Septelia Inawati Wanandi, Purnamawati Purnamawati, Alice Tamara, Karina Teja Putri, Daniel Marcellius Simadibrata

Abstract


BACKGROUND: ALDH1A1 and ALDH1A3, the most renowned isozymes of aldehyde dehydrogenase (ALDH)1, are important in regulating the pluripotency of human mesenchymal stem cells (MSCs) and cancer stem cells (CSCs). The study aimed to analyze the mRNA expression of ALDH1A1 and ALDH1A3 genes in adipose stem cells (ASCs) and umbilical cord stem cells (UCSCs) along with their correlations to Oct-4 mRNA expression. Additionally, the interaction between these proteins was also investigated using in silico study to confirm the pluripotency of both MSCs compared to human breast ALDH+ CSCs.

METHODS: This research focused on determining mRNA levels of ALDH1A1, ALDH1A3 and Oct-4 in ASCs and UCSCs using one-step qRT-PCR. The data were then normalized to those in human breast CSCs and 18S rRNA. Oct-4 gene expression was also analyzed to determine the pluripotency of ASCs and UCSCs. The protein-protein interactions were in silico analyzed using String 9.1 software.

RESULTS: Relatively, ALDH1A3 was expressed at similar level in ASCs and UCSCs, while ALDH1A1 expression level was significantly higher in ASCs compared to UCSCs. In contrast to ALDH1A3, the expressions of ALDH1A1 in both MSCs were significantly lower than breast CSCs similar to Oct-4 expressions, as also revealed by the in silico data showing the interaction between these proteins. This suggests the role of ALDH1A1 on pluripotency.

CONCLUSION: ALDH1A1 and ALDH1A3 were distinctly expressed in UCSCs and ASCs, which might be associated with unique properties of ASCs and UCSCs. This study may contribute to further research in terms of implication of ALDH1A1 and ALDH1A3 expressions towards the properties of MSCs and its application in stem cell therapy.

KEYWORDS: ALDH1A1, ALDH1A3, UCSC, ASC, breast ALDH+ CSC, Oct-4


Full Text:

PDF

References


Duan J, Cai J, Guo Y, Bian X, Yu S. ALDH1A3, a metabolic target for cancer diagnosis and therapy. Int J Cancer. 2016; 139: 965-75, CrossRef.

Yassin F. Aldehyde dehyderogenase (ALDH1A1) delineating the normal and cancer stem cells in spectral lung lesions: an immunohistochemical appraisal. Pathol Res Pract. 2016; 212: 398-409, CrossRef.

Tomita H, Tanaka K, Tanaka T, Hara A. Aldehyde dehydrogenase 1A1 in stem cells and cancer. Oncotarget. 2016; 7: 11018-32, CrossRef.

Ma I, Allan AL. The role of human aldehyde dehydrogenase in normal and cancer stem cells. Stem Cell Rev and Rep. 2011; 7: 292-306, CrossRef.

Weiss ML, Troyer DL. Stem cells in the umbilical cord. Stem Cell Rev. 2006; 2: 155-62, CrossRef.

Rusu E, Necula LG, Neagu AI, Alecu M, Stan C, Albulescu R. Current status of stem cell therapy: opportunities and limitations. Turk J Biol. 2016; 40: 955-67, CrossRef.

Bunnell BA, Flaat M, Gagliardi C, Patel B, Ripoll C. Adipose-derived stem cells: isolation, expansion and differentiation. Methods. 2008; 45: 115-20, CrossRef.

Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause DS, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006; 8: 315-7, CrossRef.

Nagamura-Inoue T, He H. Umbilical cord-derived mesenchymal stem cells: their advantages and potential clinical utility. World J Stem Cells. 2014; 6: 195-202, CrossRef.

Frese L, Dijkman E, Hoerstrup SP. Adipose tissue-derived stem cells in regenerative medicine. Transfus Med Hemother. 2016; 43: 268-74, CrossRef.

Wanandi SI, Yustisia I, Neolaka GMG, Jusman SWA. Impact of extracellular alkalinization on the survival of human CD24-/CD44+ breast cancer stem cells associated with cellular metabolic shifts. Brazilian J Med Biol Res. 2017; 50: e6538, CrossRef.

Arutyunyan I, Elchaninov A, Makarov A, Fatkhudinov T. Umbilical cord as prospective source for mesenchymal stem cell-based therapy. Stem Cells International. 2016; 2016: 6901286, CrossRef.

Witkowska-Zimny M, Walenko K. Stem cells from adipose tissue. Cell Mol Biol Lett. 2011; 16: 236-57, CrossRef.

Hu L, Hu J, Zhao J, Liu J, Ouyang W, Yang C, et al. Side-by-side comparison of the biological characteristics of human umbilical cord and adipose tissue-derived mesenchymal stem cells. Int J Mol Med. 2013; 37: 115-25, CrossRef.

Minteer D, Marra KG, Rubin JP. Adipose-derived mesenchymal stem cells: biology and potential applications. Adv Biochem Eng Biotechnol. 2013; 129: 59-71, CrossRef.

Hosea R, Hardiany NS, Ohneda O, Wanandi SI. Glucosamine decreases the stemness of human ALDH+ breast cancer stem cells by inactivating STAT3. Oncology Letters. 2018; 16: 4737-44, CrossRef.

Purnamawati, Pawitan JA, Rachman A, Liem IK, Wanandi SI. Secretomes of adipose and umbilical cord-derived stem cells affect ALDH1A1 expression in breast cancer stem cells. Adv Sci Lett. 2017; 23: 6701-4, CrossRef.

Kiefer FW, Vernochet C, O’Brien P, Spoerl S, Brown JD, Nallamshetty S, et al. Retinaldehyde dehydrogenase 1 regulates a thermogenic program in white adipose tissue. Nat Med. 2012; 18: 918-25, CrossRef.

Itoh H, Nishikawa S, Haraguchi T, Arikawa Y, Hiyama M, Eto S, et al. Aldehyde dehydrogenase activity identifies a subpopulation of canine-adipose-derived stem cells with higher differentiation potential. J Vet Med Sci. 2017; 79: 1540-4, CrossRef.

Choudehery MS, Badowski M, Muise A, Harris DT. Comparison of human mesenchymal stem cells derived from adipose and cord tissue. Cytotherapy. 2013; 15: 330-43, CrossRef.

Cojoc M, Peitzsch C, Kurth I, Trautmann F, Kunz-Schughart LA, Telegeev GD, et al. Aldehyde dehydrogenase is regulated by β-catenin/TCF and promotes radioresistance in prostate cancer progenitor cells. Cancer Res. 2015; 75:1482-94, CrossRef.

Merrill BJ. Wnt pathway regulation of embryonic stem cell selfrenewal. Cold Spring Harb Perspect Biol. 2012; 4: a007971, CrossRef.

Ring A, Kim YM, Kahn M. Wnt/catenin signaling in adult stem cell physiology and disease. Stem Cell Rev. 2014; 10: 512-25, CrossRef.

Purnamawati, Pawitan JA, Rachman A, Wanandi SI. Effects of umbilical cord- and adipose-derived stem cell secretomes on ALDH1A3 expression and autocrine TGF-b1 signaling in human breast cancer stem cells. F1000Res. 2018; 7: 249, CrossRef.

Croker AK, Rodriguez-Torres M, Xia Y, Pardhan S, Leong HS, Lewis JD, et al. Differential functional roles of ALDH1A1 and ALDH1A3 in mediating metastatic behavior and therapy resistance of human breast cancer cells. Int J Mol Sci. 2017; 18: pii: E2039, CrossRef.

Gardani M, Bertozzi N, Grieco MP, Pesce M, Simonacci F, Santi P, et al. Breast reconstruction with anatomical implants: A review of indications and techniques based on current literature. Ann Med Surg. 2017; 21: 96-104, CrossRef.

Maslova O, Novak M, Kruzliak P. Umbilical cord tissue-derived cells as therapeutic agents. Stem Cells Int. 2015; 2015: 150609, CrossRef.

Choudhery MS, Badowski M, Muise A, Harris DT. Comparison of human mesenchymal stem cells derived from adipose and cord tissue. Cytotherapy. 2013; 15: 330-43, CrossRef.

Chi Y, Han Z, Xu F, Wang Y, Feng X, Chen F, et al. Adipogenic potentials of mesenchymal stem cells from human bone marrow, umbilical cord and adipose tissue are different. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2014; 22: 588-94, CrossRef.

Brett EA, Aitzetmuller MM, Sauter MA, Machens HG, Duscher D. Breast cancer recurrence after reconstruction: know thine enemy. Oncotarget. 2018; 9: 27895-906, CrossRef.

Mazur S, Zolocinska A, Siennicka K, Janik-Kosacka K, Chrapusta A, Pojda Z. Safety of adipose-derived cell (stromal vascular fraction– SVF) augmentation for surgical breast reconstruction in cancer patients. Adv Clin Exp Med. 2018; 2018: 70798, CrossRef.

Orecchioni S, Gregato G, Martin-Padura I, Reggioani F, Braidotti P, Mancuso P, et al. Complementary populations of human adipose CD34+ progenitor cells promote growth, angiogenesis, and metastasis of breast cancer. Cancer Res. 2013; 73: 5880-91, CrossRef.

Martin-Padura I, Gregato G, Marighetti P, Mancuso P, Calleri A, Corsini C, et al. The white adipose tissue used in lipotransfer procedures is a rich reservoir of CD34+ progenitors able to promote cancer progression. Cancer Res. 2012; 72: 325-34, CrossRef.

Nowacki M, Kloskowski T, Pietkun K, Zegarski M, Pokrywczyńska M, Habib SL, et al. The use of stem cells in aesthetic dermatology and plastic surgery procedures. A compact review of experimental and clinical applications. Adv Dermatol Allergol. 2017; 34: 526-34, CrossRef.




DOI: https://doi.org/10.18585/inabj.v10i3.477

Indexed by:

                 

                  

               

     

 

The Prodia Education and Research Institute