Artemisia annua Leaf Extract Increases GLUT-4 Expression in Type 2 Diabetes Mellitus Rat
Abstract
BACKGROUND: The depletion of glucose transporter 4 (GLUT-4) affects blood glucose level in the type 2 diabetes mellitus (T2DM) patient. Herbals supplementation such as Artemisia annua is known to posses an antidiabetic potency, but its effect on long term glucose control marker, glicated hemoglobin (HbA1C) and muscle GLUT-4 expression still has not been investigated.
METHODS: Twenty Wistar rats were divided into four groups (five rats per group). The C1 group was consist of healthy control rats and C2 group was consist of diabetic control rats. Diabetic condition was induced by giving high lard fat diet for 28 days, followed with 30 mg/kgBW of Streptozotocin injection at the 29th day. Meanwhile, T1 and T2 group were consist of diabetic rats, which were supplemented with 50 mg/kgBW and 100 mg/kgBW of Artemisia annua leaf extract, respectively. GLUT-4 expression and HbA1C level were measured at the 14th day post-treatment.
RESULTS: The GLUT-4 was expressed 1.8 and two times higher in T1 and T2 group, respectively, compared to C2 group. Meanwhile, HbA1C level in C2 group was two times higher than C1 group (11.95±2.52 compared to 5.61±2.69, p<0.01). Further, the administration of 100 mg/ kgBW Artemisia annua extract caused a slight reduction of HbA1C (11.95±2.52 compared to 8.18±3.53, p>0.05)
CONCLUSION: Muscle cell’s GLUT-4 in T1 and T2 group was expressed increasingly and significantly different compared to the C2 group. HbA1C level in T2 was slightly reduced although no significant different compared to C2 group.
KEYWORDS: Artemisia annua, GLUT-4, HbA1C, type 2 diabetes mellitus
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Wild S, Rocglig G, Green A, Sicree R, King. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Dia Care. 2004; 27: 1-7, CrossRef.
Poitout V. Glucolipotoxicity of the pancreatic beta-cell: myth or reality? Biochem Soc Trans. 2008; 36: 901–4, CrossRef.
Katsumata K, Kaji Y, Takada R, Dauncey J. Nutritional regulation of GLUT expression, glucose metabolism, and intramuscular fat content in procein muscle. Asian Aust J Anim Sci. 2007; 20: 1297-304, CrossRef.
Nathan D, Buse JB, Davidson MB, Heinei RJ, Holman RR, et al. Management of hyperglicaemia in type 2 diabetes: a consensus and algorithm for initial and adjustment therapy. Diacare. 2006; 29: 1963-73, CrossRef.
Tripathi BK, Srivastava AK. Diabetes mellitus: complication and therapeutic. Med Sci Monit. 2006; 12: 130-47, PMID.
Xiao J. Phytochemicals in medicine and food. Phytochem Rev. 2015; 14: 317-20, article.
Helal EG, Abu-Aouf N, KhatabMA. Antidiabetic effect of arthemisia annua (Kaysoom) in alloxan-induced diabetic rat. Egyptian J Hospital Med. 2014; 57: 422-43, CrossRef.
Herlyanti K, Franyoto YD, Sulistyowati E. Pengaruh kombinasi ekstrak terpurifikasi herba Artemisia annua (L) dan herba andrographis paniculata (Burn f)Nees) terhadap kadar glukosa darah pada tikus resisten insulin. Jurnal Ilmu Farmasi dan Farmasi Klinik. 2014; 11: 27-31, article.
Zhang M, Xiao-Yan, Li J, Xu Z, Chen L. Research article the characterization of high-fat diet and multiple low-dose Streptozotocin induced tipe 2 diabetes rat model. Experimental Diabetes Resc. 2008: 1: 704045, CrossRef.
Granneman JG, Stricker EM. Food intake and gastric emptying I rats with Streptozotocin-induced diabetes. Am J Physiol. 1984; 247: R1054-61, CrossRef.
Sunmonu TO, Afolayan AJ. Evaluation of antidiabetic activity and associated toxicity of Artemisia afra aqueous extract in Wistar rats. Evidence-based Complement Altern Med. 2013; 2013: 929074, CrossRef.
World Health Organization. Use Glicated Hemoglobin (HbA1C) in the Diagnosis Diabetes Mellitus. Geneva: World Health Organization; 2011, PMID.
Wilson CH, Ali ES, Scrimgeour N, Martin AM, Hua J, Tallis GA, et al. Steatosis inhibits liver cell store-operated Ca2+ entry and reduces ER Ca2+ through a protein kinase C-dependent mechanism. Biochem J. 2015; 466: 379-90,
Boden G, Homko J, Barero CA, Stein TP, Chen X, Cheung P, et al. Excessive caloric intake acutely causes oxidative stress, GLUT4 carbonylation, and insulin resistance in healthy men. Sci Transl Med. 2015; 7: 304re7, CrossRef.
Watson RT, Pessin JE. Bridging the GAP between insulin signaling and GLUT4 translocation. Trends Biochem Sci. 2006; 31: 215-22, CrossRef.
Gaster M, Staehr P, Beck-Nielsen H, Schrøder HD, Handberg A. GLUT4 is reduced in slow muscle fibers of type 2 diabetic patients: is insulin resistance in type 2 diabetes a slow, type 1 fiber disease? Diabetes. 2001; 50: 1324-9, CrossRef.
Huang S, Czech MP. The GLUT-4 glucose transporter. Cell Metab. 2007; 3: 237-52, CrossRef.
Leguisamo N, Lehnen AM, Machado UF, Okamoto MM, Markoski MM, Pinto GH, et al. GLUT-4 content decreases along with insulin resistance and high levels of inflammatory markers in rats with metabolic syndrome. Cardiovasc Diabetol. 2012; 11: 100, CrossRef.
Yamato N, Ueda M, Kawabata K, Sato T, Kawasaki K, Hashimoto T, et al. Artemisia pinceps promoted glucose uptake in culture L6 muscle cell via glucose transporter 4 translocation. Biosci Biotechnol Biochem. 2010; 74: 2036-42, CrossRef.
Wang ZQ, Ribniky D, Zhang XH, Zuberi A, Raskin I, Yu Y, et al. An extract of Artemisia dracunculus L. enhances insulin receptor signaling and modulates gene expression in skeletal muscle in kkay mice. J nutr biochem. 2011; 22: 71-8, CrossRef.
Derelanko MJ. Determination of erythrocyte life span in F-344, Wistar, and Sprague-Dawley rats using a modification of the [3H] diisopropylfluorophosphate ([3H]DFP) method. Fundam Appl Toxicol. 1987; 9: 271-6, CrossRef.
Nathan DM, Turgeon H, Regan S. Relationship between glycated haemoglobin levels and mean glucose levels over time.Diabetologia. 2007; 50: 2239-44, CrossRef.
Freitas PAC, Elhert LR, Camargo JL. Glycated albumin: a potential biomarker in diabetes. Arch Endocrinol Metab. 2017; 61: 294-314, CrossRef.
Räth K, Taxis K, Walz G, Gleiter CH, Li SM, Heide L. Pharmacokinetic study of artemisinin after oral intake of a traditional preparation of Artemisia annua l. (annual wormwood). Am J Trop Med Hyg. 2004; 70: 128-32, CrossRef.
Little R, Roberts WL. A review of variant hemoglobins interfering with hemoglobin A1c measurement. J Diabetes Sci Technol. 2009; 3: 446-51, CrossRef.
Babua AV, Liub B, Gilbertc ER. Recent advances in understanding the anti-diabetic actions of dietary flavonoids. J Nutr Biochem. 2013; 24: 1777-89, CrossRef.
Ferreira JFS, Luthria DL, Sasaki T, Heyerick A. Flavonoids from Artemisia annua L. as antioxidants and their potential synergism with artemisinin against malaria and Cancer. Molecules. 2010; 15: 3135-70, CrossRef.
Song Y, Manson JE, Buring JE, Sesso HD, Liu S. Associations of dietary flavonoids with risk of type 2 diabetes, and markers of insulin resistance and systemic inflammation in women: a prospective studyand cross-sectional analysis. J Am Coll Nutr. 2005; 24: 376-84, CrossRef.
DOI: https://doi.org/10.18585/inabj.v11i1.531
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