Conditioned Media of Human Umbilical Cord Blood Mesenchymal Stem Cell Inhibits Ultraviolet B-induced Apoptosis in Fibroblasts

Dian Andriani Ratna Dewi, Ferry Sandra

Abstract


BACKGROUND: Ultraviolet (UV)B irradiated-skin cells may respond either by activating protective or apoptotic mechanisms. Several treatments has been reported to prevent apoptosis of the skin cell. To date, despite of the cell, secretome of mesenchymal stem cell (MSC) has been progressively explored for various treatment. Current research was conducted to find out the possible effect of MSC's secretome to protect normal cells from the UVB irradiation.

METHODS: For preparation of the conditioned media human umbilical cord blood mesenchymal stem cell (CM-hUCB-MSC), hUCB was collected and separated to collect mononuclear cell (MNC). MNC was cultured in MSC medium until passage 4, then checked for MSC biomarkers. Conditioned media was then produced from the cultured MSC. For induction of apoptosis, NIH3T3 cells were pretreated with/without CM-hUCB-MSC, prior to the UVB irradiation for 5-60 minutes. After 24 hours, apoptosis evaluation was conducted with Sub-G1 assay with hypotonic fluorochrome solution using flow cytometer.

RESULTS: The average of apoptotic cells irradiated with UVB for 30 minutes was 94.2%. The 30-minutes-UVB irradiation significantly induced apoptosis in NIH3T3 cells (p=0.000). The averages of apoptotic cells by pretreatment of 0, 5, 10 and 20% CM-hUCB-MSC prior to UVB irradiation were 94.2, 46.8, 31.8 and 31.5 %, respectively. The pretreatment of 5, 10 and 20% CM-hUCB-MSC prior to UVB irradiation could significantly decrease the percentage of apoptosis caused by UVB (p=0.001).

CONCLUSION: Taken together, CM-hUCB-MSC inhibited UVB-induced apoptosis in NIH3T3 cells significantly, suggesting that CM-hUCB-MSC might be a potential anti-UVB-induced apoptosis factor. Hence, further research should be explored to disclose its specific intracellular mechanism.

KEYWORDS: UVB, stem cell, conditioned media, fibroblast, apoptosis, aging, secretome, NIH3T3 cell


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References


Zaid MA, Afaq F, Syed DN, Dreher M, Mukhtar H. Inhibition of UVBmediated oxidative stress markers of photoaging in immortalized HaCaT keratinocyte by pomegranate polyphenol extract POMx. Photochem Photobiol. 2007; 83: 882-8, CrossRef.

Chao J, Yang YL, Yang Z, Tu Y, Cheng L, Chen B, et al. Perifosine sensitizes UVB-induced apoptosis in skin cells: new implication of skin cancer prevention? Cell Signal. 2012; 24: 1781-9, CrossRef.

Su Z, Yang Z, Xu Y, Chen Y, Yu Q. Apoptosis, autophagy, necroptosis, and cancer metastasis. Mol. Cancer. 2015; 14: 48, CrossRef.

Elmore S. Apoptosis: a review of programmed cell death. Toxicol Pathol. 2007; 35: 495-516, CrossRef.

Lubis AM, Sandhow L, Lubis VK, Noor A, Gumay F, Merlina M, et al. Isolation and cultivation of mesenchymal stem cells from iliac crest bone marrow for further cartilage defect management. Acta Med Indones. 2011; 43: 178-84, PMID.

Sardjono CT, Setiawan M, Frisca, Saputra V, Aniko G, Sandra F. Application of a modified method for stem cell isolation from lipoaspirates in a basic lab. Med J Indones. 2009; 18: 91-6, CrossRef.

Chouw A, Dirgantara Y, Putera BW, Sartika CR, Meutia D, Yuliana P, et al. Proliferation and characterization of Wharton-jelly's derived mesenchymal stromal cell using plasma rich platelet and plasma platelet lysate. Cytotherapy. 2017; 19: S152, CrossRef.

Sandra F, Sudiono J, Sidharta EA, Sunata EP, Sungkono DJ, Dirgantara Y, et al. Conditioned media of human umbilical cord blood mesenchymal stem cell-derived secretome induced apoptosis and inhibited growth of HeLa cells. Indones Biomed J. 2014; 6: 57-62, CrossRef.

Sandra F, Sudiono J, Binartha CTO, Chouw A, Djamil MS. Growth and osteogenic differentiation of CD117+ dental pulp and periodontal ligament cells. Indones Biomed J. 2017; 9: 78-83, CrossRef.

Aini N, Setiawan B, Sandra F. Karakteristik biologis dan diferensiasi stem cell: fokus pada mesenchymal stem cell. CDK. 2008; 35: 64-7, article.

Sandra F, Murti H, Aini N, Sardjono C, Setiawan B. Potensi terapi sel punca dalam dunia kedokteran dan permasalahannya. JKM. 2008; 8: 94-101, article.

Halim D, Murti H, Sandra F, Boediono A, Djuwantono T, Setiawan B. Stem Cell: Dasar Teori dan Aplikasi Klinis. Jakarta: Erlangga; 2010.

Laksmitawati DR, Sardjono CT, Pawitan JA, Sadikin M, Sandra F. Secretion of indoleamine 2,3-dioxygenase, an immunomodulatory substance, by adipose-derived mesenchymal stem cell. Indones J Cancer Chemoprevent. 2010; 1: 92-8, CrossRef.

Sandra F, Sidharta MA. Caffeic acid induced apoptosis in MG63 osteosarcoma cells through activation of caspases. Mol Cell Biomed Sci. 2017; 1: 28-33, CrossRef.

McDaniel D, Farris P, Valacchi G. Atmospheric skin aging-Contributors and inhibitors. J Cosmet Dermatol. 2018; 17: 124-37, CrossRef.

Salucci S, Burattini S, Battistelli M, Baldassarri V, Maltarello MC, Falcieri E. Ultraviolet B (UVB) irradiation-induced apoptosis in various cell lineages in vitro. Int J Mol Sci. 2012; 14: 532-46, CrossRef.

Luchetti F, Betti M, Canonico B, Arcangeletti M, Ferri P, Galli F, et al. ERK MAPK activation mediates the antiapoptotic signaling of melatonin in UVB-stressed U937 cells. Free Radic Biol Med. 2009; 46: 339-51, CrossRef.

Sandra F, Sidiq KM, Halim D. Terapi Stem Cell – Terobosan Kedokteran Modern untuk Penderita Gagal Jantung. Jakarta: Grasindo; 2012.

Gnecchi M, Zhang Z, Ni A, Dzau VJ. Paracrine mechanisms in adult stem cell signaling and therapy. Circ Res. 2008; 103: 1204-19, CrossRef.

Sandra F, Lahirin R. Human umbilical cord blood serum has higher potential in inducing proliferation of fibroblast than fetal bovine serum. Mol Cell Biomed Sci. 2017; 1: 65-9, CrossRef.

Wang T, Guo S, Liu X, Xv N, Zhang S. Protective effects of adiposederived stem cells secretome on human dermal fibroblasts from ageing damages. Int J Clin Exp Pathol. 2015; 8: 15739-48, PMID.

Sardjono CT, Setiawan M, Suyatna FD, Japutri I, Setiawan B, Sandra F. Immunogenicity characterization of mononucleated cells originated from umbillical cord blood. Med J Indones. 2010; 19: 14-20, CrossRef.

Wijaya MT, Sandra F. Proses dalam umbilical cord blood banking. CDK. 2007; 34: 217-20, article.

Kim WS, Park BS, Park SH, Kim HK, Sung JH. Antiwrinkle effect of adipose-derived stem cell: activation of dermal fibroblast by secretory factors. J Dermatol Sci. 2009; 53: 96-102, CrossRef.




DOI: https://doi.org/10.18585/inabj.v11i1.544

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