In vitro Antioxidant and Anti-obesity Activities of Freeze-dried Canarium sp., Averrhoa bilimbi L. and Malus domestica

Sri Utami, Susi Endrini, Said Nafik, Intan Meila Tria Lestari, Dea Anindya, Engkay Abu Bakar, Fahrul Rozy, Fathonah Fatimatuzahra Said, Ervi Afifah, Seila Arumwardana, Hayatun Nufus, Dwi Davidson Rihibiha, Hanna Sari Widya Kusuma, Satrio Haryo Benowo Wibowo, Wahyu Widowati

Abstract


BACKGROUND: The number of obesity cases is still increasing worldwide and has reached an epidemic scale. Plants are known to have a protection role in the development of obesity, however their antioxidant and anti-obesity activities have not widely known. This study was conducted to assess the in vitro antioxidant and anti-obesity activities of different types of freeze-dried fruits cultivated in Indonesia, especially Canarium sp., Averrhoa bilimbi L. and Malus domestica.

METHODS: Total phenolic content of freeze-dried fruits was identified by Folin-Ciocalteu method, while the total flavonoid content was measured by aluminium chloride colorimetric assay. To assessed the antioxidant activity, 2,2-diphenyl 1-picrylhydrazyl (DPPH) scavenging activity assay and 2,2’-azino-bis (3-ethylbenzothiazoline6-sulphonic acid) (ABTS) reducing activity assay were performed. The α-amylase and lipase inhibitory activity assay were performed to assess the anti-obesity activity. For comparison, hydroxycitric acid (HCA) compound was also assessed with DPPH, ABTS, α-amylase and lipase assays.

RESULTS: A. bilimbi had the highest total phenol content (6.35 µg GAE/mg), meanwhile M. domestica had the highest total flavonoid content (2.06 µg QE/mg). A. bilimbi also showed the highest antioxidant activity both in DPPH and ABTS assay, with inhibitory concentration (IC50)=279.99 µg/mL and 631.78 µg/mL, respectively. The freeze-dried M. domestica had the highest anti-α-amylase activity (IC50=258.85 µg/mL), while Canarium sp. had the highest anti-lipase activity (IC50=118.66 µg/mL).

CONCLUSION: Freeze-dried fruits demonstrate in vitro benefits toward obesity. A. bilimbi has potent antioxidant activity and is beneficial against obesity-related adverse health effect by relieving oxidative stress. M. domestica and Canarium sp. hamper the fat accumulation by reducing the carbohydrate absorption and dietary lipid.

KEYWORDS: antioxidant, anti-obesity, Canarium sp., Averrhoa bilimbi L., Malus domestica, hydroxycitric acid


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References


World Health Organization. Obesity: preventing and managing the global epidemic. Report of a WHO consultation. World Health Organ Tech Rep Ser. 2000; 894: 1-253, PMID.

World Health Organization. Global Status Report on Noncommunicable Diseases 2014. Geneva: World Health Organization; 2015, article.

World Health Organization [Internet]. Obesity and Overweight [updated 2018 Feb 16; cited 2018 Dec 10]. Available from: https://www.who.int/.

Hruby A, Hu FB. The epidemiology of obesity: a big picture. Pharmacoeconomics. 2015; 33: 673-89, CrossRef.

Walls HL, Backholer K, Proietto J, McNeil JJ. Obesity and trends in life expectancy. J Obes. 2012; 2012: 107989, CrossRef.

Wyatt SB, Winters KP, Dubbert PM. Overweight and obesity: prevalence, consequences, and causes of a growing public health problem. Am J Med Sci. 2006; 331: 166-74, CrossRef.

Marseglia L, Manti S, D’Angelo G, Nicotera A, Parisi E, Di Rosa G, et al. Oxidative stress in obesity: a critical component in human diseases. Int J Mol Sci. 2014; 16: 378–400, CrossRef.

Savini I, Catani M, Evangelista D, Gasperi V, Avigliano L. Obesity-associated oxidative stress: strategies finalized to improve redox state. Int J Mol Sci. 2013; 14: 10497–538, CrossRef.

Slanc P, Doljak B, Kreft S, Lunder M, Janeš D, Štrukelj B. Screening of selected food and medicinal plant extracts for pancreatic lipase inhibition. Phyther Res. 2009; 23: 874–7, CrossRef.

Boqué N, Campión J, de la Iglesia R, de la Garza AL, Milagro FI, San Román B, et al. Screening of polyphenolic plant extracts for anti-obesity properties in Wistar rats. J Sci Food Agric. 2013; 93: 1226–32, CrossRef.

Sagar VR, Suresh Kumar P. Recent advances in drying and dehydration of fruits and vegetables: a review. J Food Sci Technol. 2010; 47: 15–26, CrossRef.

Sanchez V, Baeza R, Chirife J. Comparison of monomeric anthocyanins and colour stability of fresh, concentrate and freezedried encapsulated cherry juice stored at 38°C. J Berry Res. 2015; 5: 243–51, CrossRef.

Ivanova D, Gerova D, Chervenkov T, Yankova T. Polyphenols and antioxidant capacity of Bulgarian medicinal plants. J Ethnopharmacol. 2005; 96: 145–50, CrossRef.

Widowati W, Rani AP, Hamzah RA, Arumwardana S, Afifah E, Kusuma HSW, et al. Antioxidant and antiaging assays of Hibiscus sabdariffa extract and its compounds. Nat Prod Sci. 2017; 23: 192–200, CrossRef.

Sudha G, Priya MS, Shree RI, Vadivukkarasi S. In Vitro Free Radical Scavenging Activity of Raw Pepino Fruit (Solanum muricatum Aiton.). Int J Curr Pharm Res. 2011; 3: 137–40, article.

Widowati W, Wargasetia TL, Afifah E, Mozef T, Kusuma HSW, Nufus H, et al. Antioxidant and antidiabetic potential of Curcuma longa and its compounds. Asian J Agri Biol. 2018; 6: 149–161, article.

Etoundi C, Kuaté D, Ngondi J, Oben J. Anti-amylase, anti-lipase and antioxidant effects of aqueous extracts of some Cameroonian spices. J Nat Prod. 2010; 3: 165–71, article.

Widowati W, Fauziah N, Herdiman H, Afni M, Afifah E, Kusuma HSW, et al. Antioxidant and anti aging assays of oryza sativa extracts, vanillin and coumaric acid. J Nat Remedies. 2016; 16: 88, CrossRef.

Adnyana IK, Abuzaid AS, Iskandar EY, Kurniati NF. Pancreatic lipase and α-amylase inhibitory potential of mangosteen (Garcinia mangostana Linn.) pericarp extract. Int J Med Res Heal Sci. 2016; 5: 23, CrossRef.

Bhutkar MA. In vitro studies on alpha amylase inhibitory activity of some indigenous plants. Mod Appl Pharm Pharmacol. 2018; 2018: 1-5, CrossRef.

Liu S, Li D, Huang B, Chen Y, Lu X, Wang Y. Inhibition of pancreatic lipase, α-glucosidase, α-amylase, and hypolipidemic effects of the total flavonoids from Nelumbo nucifera leaves. J Ethnopharmacol. 2013; 149: 263–9, CrossRef.

Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem. 2001; 49: 5165–70, CrossRef.

Sulaiman CT, Sadashiva CT, George S, Goplakrishnan VK, Balachandran I. Chromatographic studies and in vitro screening for acetyl cholinesterase inhibition and antioxidant activity of three acacia species from South India. Anal Chem Lett. 2013; 3: 111–8, CrossRef.

Panche AN, Diwan AD, Chandra SR. Flavonoids: an overview. J Nutr Sci. 2016; 5: e47, CrossRef.

Tsang C, Higgins S, Duthie GG, Duthie SJ, Howie M, Mullen W, et al. The influence of moderate red wine consumption on antioxidant status and indices of oxidative stress associated with CHD in healthy volunteers. Br J Nutr. 2005; 93: 233–40, CrossRef.

Benzie IFF, Szeto YT, Strain JJ, Tomlinson B. Consumption of green tea causes rapid increase in plasma antioxidant power in humans. Nutr Cancer. 1999; 34: 83–7, CrossRef.

Stalmach A, Steiling H, Williamson G, Crozier A. Bioavailability of chlorogenic acids following acute ingestion of coffee by humans with an ileostomy. Arch Biochem Biophys. 2010; 501: 98–105, CrossRef.

Ottaviani JI, Borges G, Momma TY, Spencer JPE, Keen CL, Crozier A, et al. The metabolome of [2-14C](−)-epicatechin in humans: implications for the assessment of efficacy, safety and mechanisms of action of polyphenolic bioactives. Sci Rep. 2016; 6: 29034, CrossRef.

Orak H, Aktas T, Yagar H, İsbilir SS, Ekinci N, Sahin FH. Effects of hot air and freeze drying methods on antioxidant activity, colour and some nutritional characteristics of strawberry tree (Arbutus unedo L) fruit. Food Sci Technol Int. 2012; 18: 391–402, CrossRef.

Hasanuzzaman M, Ali MR, Hossain M, Kuri S, Islam MS. Evaluation of total phenolic content, free radical scavenging activity and phytochemical screening of different extracts of Averrhoa bilimbi (fruits). Int Curr Pharm J. 2013; 2: 92–6, CrossRef.

Mannan A, Rahman MM, Habib MR, Hasan MA, Al Amin M, Saha A. Comparative assessment on in vitro antioxidant activities of ethanol extracts of Averrhoa bilimbi, Gymnema sylvestre and Capsicum frutescens. Pharmacognosy Res. 2014; 6: 36-41, CrossRef.

Navarro M, Moreira I, Arnaez E, Quesada S, Azofeifa G, Vargas F, et al. Polyphenolic characterization and antioxidant activity of Malus domestica and Prunus domestica cultivars from Costa Rica. Foods. 2018; 7: 15, CrossRef.

Kschonsek J, Wolfram T, Stöckl A, Böhm V. Polyphenolic compounds analysis of old and new apple cultivars and contribution of polyphenolic profile to the in vitro antioxidant capacity. Antioxidants. 2018; 7: 20, CrossRef.

Papetti A, Daglia M, Gazzani G. Anti- and pro-oxidant water soluble activity of Cichorium genus vegetables and effect of thermal treatment. J Agric Food Chem. 2002; 50: 4696–704, CrossRef.

Higuchi M, Dusting GJ, Peshavariya H, Jiang F, Hsiao STF, Chan EC, et al. Differentiation of human adipose-derived stem cells into fat involves reactive oxygen species and forkhead Box O1 mediated upregulation of antioxidant enzymes. Stem Cells Dev. 2013; 22: 878–88, CrossRef.

Lee H, Lee YJ, Choi H, Ko EH, Kim J. Reactive oxygen species facilitate adipocyte differentiation by accelerating mitotic clonal expansion. J Biol Chem. 2009; 284: 10601–9, CrossRef.

Furukawa S, Fujita T, Shimabukuro M, Iwaki M, Yamada Y, Nakajima Y, et al. Increased oxidative stress in obesity and its impact on metabolic syndrome. J Clin Invest. 2004; 114: 1752–61, CrossRef.

Hogan S, Canning C, Sun S, Sun X, Zhou K. Effects of grape pomace antioxidant extract on oxidative stress and inflammation in diet induced obese mice. J Agric Food Chem. 2010; 58: 11250–6, CrossRef.

Barrett ML, Udani JK. A proprietary alpha-amylase inhibitor from white bean (Phaseolus vulgaris): A review of clinical studies on weight loss and glycemic control. Nutr J. 2011; 10: 24, CrossRef.

Kwon YI, Apostolidis E, Shetty K. Inhibitory potential of wine and tea against α-amylase and α-glucosidase for management of hyperglycemia linked to type 2 diabetes. J Food Biochem. 2008; 32: 15–31, CrossRef.

Foster-Schubert KE, Cummings DE. Emerging therapeutic strategies for obesity. Endocr Rev. 2006; 27: 779–93, CrossRef.

Yun JW. Possible anti-obesity therapeutics from nature – A review. Phytochemistry. 2010; 71: 1625–41, CrossRef.

Yuda N, Tanaka M, Suzuki M, Asano Y, Ochi H, Iwatsuki K. Polyphenols extracted from black tea (Camellia sinensis) residue by hot-compressed water and their inhibitory effect on pancreatic lipase in vitro. J Food Sci 2012; 77: H254–61, CrossRef.




DOI: https://doi.org/10.18585/inabj.v11i3.728

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