Microbiological and Biochemical Contamination Analysis of Refilled Drinking-water in Abeli, Kendari, Southeast Sulawesi

Juminten Saimin, Hartati Hartati, Yenti Purnamasari, Sufiah Asri Mulyawati, Tien Tien, Pranita Ayitrina

Abstract


BACKGROUND: The access to safe drinkingwater is increasingly difficult, especially in water catchment or coastal areas. Due to the difficulty, people in coastal areas tend to consume ready-to-use refilled drinking-water. However, the sanitation facilities on most drinking-water refill depots do not meet the requirements, hence really prone to microbial contamination. We conducted this study to determine the types of bacteria causing the contamination of refilled drinking-water from refill depots in Abeli, a coastal area in Kendari.

METHODS: Samples were collected from all 6 drinkingwater refill depots in Abeli. Three-series fermentation tubes were used for the microbiological test, including the presumptive, confirmative and completed tests. The biochemical test was performed with indole test, methyl red test, Voges-Proskauer test, and citrate utilization test (IMViC) methods and the triple sugar iron agar (TSIA) test.

RESULTS: Among 6 refilled drinking-water samples, we found the growth of coliform bacterial colonies in 3 samples, exhibiting various characteristics. Based on the characteristics, both microbiological and biochemical, the identified bacteria were Proteus sp., Escherichia coli and Klebsiella oxytoca.

CONCLUSION: Bacteria that contaminated refilled drinking-water in Abeli were Proteus sp., E. coli and K. oxytoca. Continuous supervision is required to ensure the quality of water from drinking-water refill depots.

KEYWORDS: bacteria, Proteus sp., Escherichia coli, Klebsiella oxytoca, coastal areas, refilled drinking water


Full Text:

PDF

References


Montgomery KS. Nutrition column an update on water needs during pregnancy and beyond. J Perinat Educ. 2002; 11: 40-2, CrossRef.

Cunningham FG, Leveno KJ, Bloom SL, Dashe JS, Hoffman BL, Casey BM, et al. Williams Obstetrics. 24th edition. New York: McGraw-Hill; 2013, NLMID.

World Health Organization. Guideline for Drinking-water Quality. 4th edition. Geneva: World Health organization; 2011, article.

World Health Organization. Guideline for Drinking-Water Quality: Incorporating 1st and 2nd addenda, Vol.1, Recommendations. 3rd edition. Geneva; World Health organization; 2008, article.

Kristiyanti M. Empowerment of coastal communities through ICZM approach (Integrated Coastal Zone Management). Proceedings of the National Conference Multi Discipline Science. 2016 Jul 28, Semarang. 2016; p.752-60.

Tjampakasari CR, Wahid MH. Water quality of Angke River: microbiological of view. Med J Indones. 2008; 17; 82-7, CrossRef.

Research and Development Center of the Republic of Indonesian Ministry of Health. Basic Health Research. Jakarta: Ministry of Health Republic of Indonesia; 2013, article.

BSN. Bottled Water, Indonesian National Standard, SNI 01-3553-2006. Jakarta: National Standards Agency (BSN); 2006.

Kemenkes RI. Regulation of the Minister of Health of the Republic of Indonesia No.492 / MENKES / Per / IV / 2010 concerning Drinking Water Quality Requirements. Jakarta: Ministry of Health Republic of Indonesia; 2010.

Dirjen PP-PL. Guidelines for Implementing Drinking Water Depot Hygiene Hygiene. Jakarta: Directorate General of PP & PL Republic of Indonesia Ministry of Health (Dirjen PP-PL); 2010.

Saimin J, Ristanti T, Hartati. The quality of drinking water refills in the coastal areas of Kendari City. Proceedings of the National Conference on The Role of Health Workers in Resistance Prevention Programs Antimicrobial (PPRA). 2019 Feb 14, Kendari. 2019; p.18.

Puspitasari E. Analysis of the factors affecting the quality of the chemical and microbiological drinking water at the drinking water refill depot in Tulung Agung District. J Quality Pub Health. 2018; 1; 104-12, CrossRef.

Bartram J, Pedley S. Microbiological analysis. In: Bartram J, Balance R, editor. Water Quality Monitoring - A Practical Guide to the Design and Implementation of Freshwater Quality Studies and Monitoring Programmes. Geneva: UNEP/WHO; 1996. p.1-27.

Sridhar Rao [Internet]. IMViC Reactions [updated 2006 Jun; cited 2019 May 10]. Available from: https://www.microrao.com/.

Microbeonline [Internet]. Triple Sugar Iron Agar (TSI): Principle, Procedure and Interpretation [updated 2013 Jul 16; cited 2019 May 10]. Available from: https://microbeonline.com/.

Garrity GM, Bell JA, Lilburn TG. Taxonomic outline of the prokaryotes. Bergey’s Manual of Systematic Bacteriology. 2nd edition. New York: Springer; 2004, NLMID.

Radji M, Oktavia H, Suryadi H. Bacteriological examination of refilled drinking water in some of the refilled drinking water depots in the area of Lenteng Agung and Srengseng Sawah, Jakarta Selatan. Majalah Ilmu Kefarmasian. 2008; 5: 101-9.

Rajendra S, Rubin D, Abhishek M. Microbiological quality of potable water in Dehradun City. IRJP. 2012; 6: 130-7, article.

Microbeonline [Internet]. Triple Sugar Iron Agar (TSI): Principle, Procedure and Interpretation [updated 2013 Jul 16; cited 2019 May 10]. Available from: https://microbeonline.com/.




DOI: https://doi.org/10.18585/inabj.v12i2.871

Indexed by:

                 

                  

               

     

 

The Prodia Education and Research Institute