Costus Root Extract Preserves Thyroid Hormones Levels,  Thyroglobulin Expression and Thyroid Tissues in  Rats Receiving Valproate Sodium

Marwa Salah Mahmoud

doi:10.18585/inabj.v12i4.1145

Costus Root Extract Preserves Thyroid Hormones Levels, Thyroglobulin Expression and Thyroid Tissues in Rats Receiving Valproate Sodium

Marwa Salah Mahmoud

Abstract

BACKGROUND: Valproate sodium is an anticonvulsant drug. Saussurea lappa (costus) is a medicinal plant rich with antioxidants. This research aimed to assess the protective effect of costus root extract against valproate sodium-induced thyrotoxicity.

METHODS: Eighty adult male albino rats were equally divided into four groups; group I: untreated control, group II: rats were given 200 mg/kg BW valproate sodium orally and daily for 8 weeks, group III: rats were given 300 mg/kg BW costus root extract orally and daily for 8 weeks, and group IV: rats were given combination of valproate sodium and costus root extract. After 8 weeks, blood samples were collected to evaluate T3, T4 and thyroid-stimulating hormone (TSH) levels. Thyroid gland samples were handled for light and electron microscopic investigation. The heights of follicular cells, area % of collagen fibers and color intensity of thyroglobulin immunoreaction were statistically analyzed.

RESULTS: After being given valproate sodium as an induction, hormonal assay showed significant decrease in serum T3 and T4 and significant increase of TSH. Follicular and cellular alterations were shown by light and electron microscopes. Morphometric study revealed increased follicular cell height and area % of collagen fibers and decreased color intensity of thyroglobulin. In contrast, costus root extract appeared to have a protective role against valproate sodium-induced thyroid injury. Most of the changes induced by valproate sodium were not observed after supplementation with the plant root extract.

CONCLUSION: Valproate sodium has serious effects on the function and structure of thyroid gland, and this study shows that costus root extract could have a protective effect against these effects.

KEYWORDS: valproate sodium, rat, thyrotoxicity, Saussurea lappa

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References

Panayiotopoulos CP, editor. A Clinical Guide to Epileptic Syndromes and their Treatment. United Kingdom: Springer Healthcare; 2010.

Ibrahim MA. Evaluation of hepatotoxicity of valproic acid in albino mice, histological and histoistochemical studies. Life Sci J. 2012; 9: 153-9, article.

Hamouda MH, Abdel Aal FS, El-Mashad FH. Effect of sodium valproate on the structure of the renal cortex of adult male Albino rat and the role of cinnamon. Al-Azhar Med J. 2019; 48: 1-28, article.

Shakya RD, Hoque MK, Sapkota AS, Gupta PK. Differential hepatotoxic effects of sodium valproate at different doses in albino rats. Kathmandu Univ Med J. 2018; 61: 78-82, PMID.

Hamed SH. The effect of anti-epileptic drugs on thyroid hormonal function: causes and implications. Expert Rev Clin Pharmacol. 2015; 8: 741-50, CrossRef.

Ercegovac M, Jovi N, Simi T, Besla-Bumbairevi L, Soki D, Savi-Radojevi A, et al. Antiepileptic drugs affect protein, lipids and DNA oxidative damage and antioxidant defense in patients with epilepsy. J Med Biochem. 2013; 32: 121-30, CrossRef.

Rogawski MA, Löscher W, Rho JM. Mechanisms of action of antiseizure drugs and the ketogenic diet. Cold Spring Harb Perspect Med. 2016; 6: a022780, CrossRef.

Sepahi S, Riahi-Zangani B, Ghorani-Azam A. Effect of valproic acid on metabolic status and endocrine system in pediatric patients with epilepsy: systematic literature review. Rev Clin Med. 2017; 4: 7-13, article.

Elbaramawi MI. Effect of anti-epileptic drugs on the thyroid gland in adult Albino rats: hormonal assay, histological and morphometric study. Egypt J Histol. 2014; 38: 10-20, CrossRef.

Abdul-Hamid M, Salah M. Intervention of ginger or propolis ameliorates methotrexate-induced ileum toxicity. Toxicol Ind Health. 2016; 32: 313-22, CrossRef.

Bolkiny Y, Tousson E, El-Atrsh A, Akela M, Farg M. Costus root extract alleviates blood biochemical derangements of experimentally-induced hypo- and hyperthyroidism in mice. Annu Res Rev Biol. 2019; 31: 1-10, CrossRef.

Srivastava S, Singh P, Jha KK, Mishra G, Srivastava S, Khosa RL. Evaluation of anti-arthritic potential of the methanolic extract of the aerial parts of Costus speciosus. J Ayurveda Integr Med. 2012; 3: 204-8, CrossRef.

Töllner K, Wolf S, Löscher W, Gernert M. The anticonvulsant response to valproate in kindled rats is correlated with its effect on neuronal firing in the substantia nigra pars reticulata: a new mechanism of pharmacoresistance. J Neurosci Res. 2011; 31: 16423-34, CrossRef.

Vogel GH, Vogel WH. Drug Discovery and Evaluation. Berlin: Spinger Verlag; 1998.

Smals AGH, Ross AH, Kloppenborg PWC. Dichotomy between serum free triiodothyronine and free thyroxine concentrations in familial thyroxine-binding globulin deficiency. J Clin Endocrinol. Metab. 1981; 53: 917-22, CrossRef.

Larsen PR. Thyroid–pituitary interaction: feedback regulation of thyrotropin secretion by thyroid hormones. N Engl J Med. 1982; 306: 23-32, CrossRef.

Mandel SJ, Brent GA, Larsen PR. Levothyroxine therapy in patients with thyroid disease. Ann Intern Med. 1993; 119: 492-502, CrossRef.

Bancroft J, Gamble M. Theory and Practice of Histolological Technique. 5th edition. London: Churchil Livingstone; 2002, NLMID.

Pearse AG. Histochemistry, Theoretical and Applied Vol. 11. 3rd edition. Boston: Little Brownand; 1972, NLMID.

Ramos-Vara JA, Beissenherz ME. Optimization of immunohistochemical methods using two different antigen retrieval methods on formalin-fixed, paraffin-embedded tissues: Experience with 63 markers. J Vet Diagn Invest. 2000; 12: 307-11, CrossRef.

Wang YF, Liu B, Fan XS, Rao Q, Xu Y, Xia QY, et al. Thyroid carcinoma showing thymus-like elements: A clinicopathologic, immunohistochemical, ultrastructural and molecular analysis. Amer J Clin Path. 2015; 143: 223-33, CrossRef.

Bozzola JJ, Rusell LD. Electron Microscopy Principles and Techniques for Biologists. 2nd edition. Boston: Jones and Bartlett Publisher; 1999, NLMID.

Glauert AM, Lewis PR. Biological Specimen Preparation for Transmission Electron Microscopy. Vol. 17. London: Portland Press; 1998, NLMID.

Røste LS, Taubøll E, Mørkrid L, Bjørnenak T, Saetre ER, Mørland T, et al. Antiepileptic drugs alter reproductive endocrine hormones in men with epilepsy. Eur J Neurol. 2005; 12: 118-24, CrossRef.

Eirís-Puñal J, Del Río-Garma M, Del Río-Garma MC, Lojo-Rocamonde S, Novo-Rodríguez I, Castro-Gago M. Long-term treatment of children with epilepsy with valproate or carbamazepine Corp; 2011.may cause subclinical hypothyroidism. Epilepsia. 1999; 40: 1761-6, CrossRef.

Benedetti MS, Whomsley R, Baltes E, Tonner F. Alteration of thyroid hormone homeostasis by anti-epileptic drugs in humans: Involvement of glucuronosyltransferase induction. Eur J Clin Pharmacol. 2005; 61: 863-72, CrossRef.

Verrotti A, Scardapane A, Manco R, Chiarelli F. Antiepileptic drugs and thyroid function. J Pediatr Endocrinol Metab. 2008; 21: 401-8, CrossRef.

Castro-Gago M, Novo-Rodrı´guez MI, Go´mez-Lado C, Rodrı´guez-Garcı´a J, Rodrı´guez-Segade S, Eirı´s-Pun˜al J. Evolution of subclinical hypothyroidism in chil-dren treated with anti-epileptic drugs. Pediatr Neurol. 2007; 37: 426-30, CrossRef.

Sibarani JJ, Deliana M, Saing JH. Valproate use and thyroid dysfunction in children with idiopathic epilepsy. Paediatrica Indonesiana. 2018; 58: 192-7, CrossRef.

Wier FA, Farley CL. Clinical controversies in screening women for thyroid disorders during pregnancy. J Midwifery Womens Health. 2006; 51: 152-8, CrossRef.

Ganong WF. Review of Medical Physiology. 22nd edition. New York: McGraw-Hill; 2005.

Mahmoud T, Qureshi IZ, Iqabi MJ. Histopathological and biochemical changes in rat thyroid following acute exposure to hexavalent chromium. Histol Histopathol. 2010; 25: 1355-70, CrossRef.

Rodrigo R, Trujillo S, Basco C, Orellena M, Thielemann L, Araya J. Changes in (Na+K)-adenosine triphosphate activity and ultrastructure of lung and kidney associated with oxidative stress induced by acute ethanol intoxication. Chest. 2002; 121: 589-96, CrossRef.

Teng X, Shan Z, Teng W, Fan C, Wang H, Guo R. Experimental study on the effects of chronic iodine excess on thyroid function, structure, and autoimmunity in autoimmune-prone NOD.H-2h4 mice. Clin Exp Med. 2009; 9: 51-9, CrossRef.

Kim I, Xu W, Reed JC. Cell death and endoplasmic reticulum stress: Disease relevance and therapeutic opportunities. Nat Rev Drug Discov. 2008; 7: 1013-30, CrossRef.

Rajkovic V, Matavulj M, Johansson O. Light and electron microscopic study of the thyroid gland in rats exposed to power-frequency electromagnetic fields. J Exp Biol. 2006; 209: 3322-8, CrossRef.

Alnahdi HS. Injury in metabolic gland induced by pyrethroid insecticide could be reduced by aqueous extract of Sassura lappa.Int J Pharm Res Allied Sci. 2017; 6: 86-97, article.

Rahmana MA, Hongb J, Huha S. Antiproliferative properties of Saussurea lappa Clarke root extract in SH-SY5Y neuroblastoma cells via intrinsic apoptotic pathway. Anim Cells Syst. 2015; 19: 119-26, CrossRef.

DOI: https://doi.org/10.18585/inabj.v12i4.1145

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