Robusta Extract Cream Ameliorated Ultraviolet B-induced Wrinkle Skin of Mice by the Regulation of Epidermal Thickness and Inhibition of MMP-1

Dimpuulina Erna Mariati, Sunarjati Sudigdoadi, Ronny Lesmana, Astrid Feinisa Khairani, Julia Windi Gunadi, Vita Murniati Tarawan, Unang Supratman, Hanna Goenawan


BACKGROUND: Recently, coffee is widely used for preventing photoaging because of its antioxidant capacity. Among two kinds of coffee, robusta coffee has higher content of antioxidant such as chlorogenic acid (CGA) and caffeine. Researchs about robusta coffee bean effect on photoaging due to UVB radiation is still limited. Therefore, the aim of this study was to examine the effect of robusta extract cream (RE cream) on preventing wrinkle in mice induced by ultraviolet-B (UVB) radiation.

METHODS: RE cream was made by mixing RE coffee with moisturizing cream in different concentration (10%, 20%, and 40%). Twenty-five male of Mus musculus Balb/c strain mice aged 4 weeks were divided into five groups; control group, UVB group, UVB + 10% RE group, UVB + 20% RE group, and UVB + 40% RE group. The UVB groups were given UVB radiation three times a week with an exposure duration of 100 seconds per time for ten weeks. At the end of the treatment, skin samples were excised and statined histologically, also were analyzed for their protein expression. Evaluation of wrinkles was carried out using the Bissete method before and after treatment. To evaluate the thickness of the epidermis, HE staining was performed, while masson Trichome staining was performed to determine the collagen content.

RESULTS: RE cream-treated groups showed lower wrinkle score compared to the control group. Furthermore, in UVB + 10% RE group, the RE cream application reduce wrinkle formation. In UVB + 10% RE group and UVB + 20% RE group, the RE cream application increased epidermal thickness and collagen content (p=0.00). While collagenase, matrix metalloproteinase-1 (MMP-1) expression was lower in UVB + 20% RE group compared to the UVB group (p<0.05), however the MMP1 expression in UVB + 40% RE group was higher than other treatment group.

CONCLUSION: RE cream prevents wrinkle by maintaining epidermal thickness and collagen contain. RE cream also decreases MMP-1 expression in mice.

KEYWORDS: coffee, collagen, MMP-1, robusta, wrinkle

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Hani Y, Sharma S. Anatomy, Skin (Integument), Epidermis. In: StatPearls. Treasure Island (FL): StatPearls Publishing LLC; 2019. p.1-12, NLMID.

Panich U, Sittithumcharee G, Rathviboon N, Jirawatnotai S. Ultraviolet radiation-induced skin aging: the role of DNA damage and oxidative stress in epidermal stem cell damage mediated skin aging. Stem Cells Int. 2016; 2016: 7370642, CrossRef.

Addor F. Beyond photoaging: additional factors involved in the process of skin aging. Clin Cosmet Investig Dermatol. 2018; 11: 437-43, CrossRef.

Shanbhag S, Nayak A, Narayan R, Nayak UY. Anti-aging and Sunscreens : Paradigm Shift in Cosmetics. Adv Pharm Bull. 2019; 9: 348-59, CrossRef.

Pittayapruek P, Meephansan J, Prapapan O, Komine M, Ohtsuki M. Role of matrix metalloproteinases in Photoaging and photocarcinogenesis. Int J Mol Sci. 2016; 17: 868, CrossRef.

Liebel F, Kaur S, Ruvolo E, Kollias N, Southall MD. Irradiation of skin with visible light induces reactive oxygen species and matrix-degrading enzymes. J Invest Dermatol. 2012; 132: 1901-7, CrossRef.

Han S, Makareeva E, Kuznetsova NV., DeRidder AM, Sutter MB, Losert W, et al. Molecular mechanism of type I collagen homotrimer resistance to mammalian collagenases. J Biol Chem. 2010; 285: 22276-81, CrossRef.

Rahunen K, Rieppo L, Lehenkari P, Finnilä M, Saarakkala S. Evaluation of quantitativity of histological collagen stains in articular cartilage. Osteoarthr Cartil. 2016; 24: S307-8.

Garg SK. Green coffee bean. Nutraceuticals Effic Saf Toxic. 2016; 62: 653-67.

Jeszka-Skowron M, Sentkowska A, Pyrzyńska K, De Peña MP. Chlorogenic acids, caffeine content and antioxidant properties of green coffee extracts: influence of green coffee bean preparation. Eur Food Res Technol. 2016; 242: 1403-9, CrossRef.

Bessada SMF, Alves RC, Oliveira BPP. Coffee silverskin: A review on potential cosmetic applications. Cosmetics. 2018; 5: 5, CrossRef.

Tajik N, Tajik M, Mack I, Enck P. The potential effects of chlorogenic acid, the main phenolic components in coffee, on health: a comprehensive review of the literature. Eur J Nutr. 2017; 56: 2215-44, CrossRef.

Liang N, Kitts DD. Role of chlorogenic acids in controlling oxidative and inflammatory stress conditions. Nutrients. 2015; 8: 16, CrossRef.

Choi HS, Park ED, Park Y, Suh HJ. Spent coffee ground extract suppresses ultraviolet B-induced photoaging in hairless mice. J Photochem Photobiol B Biol. 2015; 153: 164-72, CrossRef.

Pergolizzi S, D’Angelo V, Aragona M, Dugo P, Cacciola F, Capillo G, et al. Evaluation of antioxidant and anti-inflammatory activity of green coffee beans methanolic extract in rat skin. Nat Prod Res. 2018; 34: 1535-1541, CrossRef.

Eltania F, Lesmana R, Sudigdoadi S, Sudigdoadi S, Khairani AF, Goenawan H, et al. Tranexamic acid cream protects ultraviolet B-induced photoaging in Balb/c mice skin by increasing mitochondrial markers: changes lead to improvement of histological appearance. Photochem Photobiol. 2020; 96: 863-9, CrossRef.

Bissett DL, Hannon DP, Orr T V. An animal model of solar-aged skin: histological, physical, and visible changes in UV-irradiated hairless mouse skin. Photochem Photobiol. 1987; 46: 367-78, CrossRef.

Chiu HW, Chen CH, Chen YJ, Hsu YH. Far-infrared suppresses skin photoaging in ultraviolet B-exposed fibroblasts and hairless mice. PLoS One. 2017; 12: 1-15, CrossRef.

Yin R, Chen Q, Hamblin MR, Yin R. Skin aging and photoaging. In: Skin photoaging. San Rafael, CA: Morgan & Claypool Publishers; 2015. p.1–4, NLMID.

Debacq-Chainiaux F, Leduc C, Verbeke A, Toussaint O. UV, stress and aging. Dermatoendocrinol. 2012; 4: 236-40, CrossRef.

Chiang HM, Lin TJ, Chiu CY, Chang CW, Hsu KC, Fan PC, et al. Coffea arabica extract and its constituents prevent photoaging by suppressing MMPs expression and MAP kinase pathway. Food Chem Toxicol. 2011; 49: 309-18, CrossRef.

Dias R, Benassi M. Discrimination between arabica and robusta coffees using hydrosoluble compounds: is the efficiency of the parameters dependent on the roast degree? Beverages. 2015; 1: 127-39, CrossRef.

Fukagawa S, Haramizu S, Sasaoka S, Yasuda Y, Tsujimura H, Murase T. Coffee polyphenols extracted from green coffee beans improve skin properties and microcirculatory function. Biosci Biotechnol Biochem. 2017; 81: 1814-22, CrossRef.

Stefanello N, Spanevello RM, Passamonti S, Porciúncula L, Bonan CD, Olabiyi AA, et al. Coffee, caffeine, chlorogenic acid, and the purinergic system. Food Chem Toxicol. 2019; 123: 298-313, CrossRef.

Tanaka K, Asamitsu K, Uranishi H, Iddamalgoda A, Ito K, Kojima H, et al. Protecting skin photoaging by NF-κB inhibitor. Curr Drug Metab. 2010; 11: 431-5, CrossRef.

Freitas-Rodríguez S, Folgueras AR, López-Otín C. The role of matrix metalloproteinases in aging: Tissue remodeling and beyond. Biochim Biophys Acta - Mol Cell Res. 2017; 1864: 2015-25, CrossRef.

Shin JW, Kwon SH, Choi JY, Na JI, Huh CH, Choi HR, et al. Molecular mechanisms of dermal aging and antiaging approaches. Int J Mol Sci. 2019; 20: 2126, CrossRef.

Choi HS, Park ED, Park Y, Han SH, Hong KB, Suh HJ. Topical application of spent coffee ground extracts protects skin from ultraviolet B-induced photoaging in hairless mice. Photochem Photobiol Sci. 2016; 15: 779-90, CrossRef.

Choi HJ, Alam MB, Baek ME, Kwon YG, Lim JY, Lee SH. Protection against UVB-Induced Photoaging by Nypa fruticans via Inhibition of MAPK/AP-1/MMP-1 Signaling. Oxid Med Cell Longev. 2020; 2020: 2905362, CrossRef.

Du WY, Xiao Y, Yao JJ, Hao Z, Zhao YB. Involvement of NADPH oxidase in high-dose phenolic acid-induced pro-oxidant activity on rat mesenteric venules. Exp Ther Med. 2017; 13: 17-22, CrossRef.


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