Antihypercholesterolemic and Antioxidant Effects of Blumea balsamifera L. Leaf Extracts to Maintain Luteinizing Hormone Secretion in Rats Induced by High-Cholesterol Diets
Abstract
BACKGROUND: The discovery of herbal ingredients for antihypercholesterolemic and to improve male reproductive function is very necessary due to high-cholesterol diet factors. Blumea balsamifera leaf extract (BBLE) is known to be able to increase the number and diameter of Leydig cells in rats given high-fat feed. This study was to conducted to determine the levels of total cholesterol, body weight, plasma malondialdehyde (MDA), Superoxide Dismutase (SOD), Luteinizing Hormone (LH) in high-cholesterol-fed male rats provided with BBLE.
METHODS: This research utilized a randomized post-test only control group. Ethanol was used to extract the BBLE, which was then evaporated. For 21 days, 16 Wistar rats were given a high-cholesterol diet. To determine the effect of BBLE on the high-cholesterol diet, the samples were divided into two groups (control and BBLE group) on day 22. The treatments lasted 30 days. SOD, plasma MDA, LH, and total cholesterol were measured.
RESULTS: The results showed that the SOD and LH parameters were significantly higher in the treatment of BBLE compared to the control group (p<0.05). The parameters of total cholesterol levels, bodyweight, and MDA of rats given BBLE were significantly lower than those of the control group (p<0.05).
CONCLUSION: Our findings highlight that BBLE has antihypercholesterolemic and antioxidant effects. The BBLE also has potential to be used as a therapy to maintain male reproductive function because it has a positive effect on the hypothalamic-pituitary axis through increasing LH secretion.
KEYWORDS: Malondialdehyde, Superoxide dismutase, Luteinizing hormone, Blumea balsamifera extract, High-cholesterol diet
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Lykkesfeldt J. Malondialdehyde as biomarker of oxidative damage to lipids caused by smoking. Clin Chim Acta. 2007; 380: 50-8, CrossRef.
Kumar R, Venkatesh S, Kumar M, Tanwar M, Shasmsi MB, Kumar R, et al. Oxidative stress and sperm mitochondrial DNA mutation in idiopathic oligoasthenozoospermic men. Indian J Biochem Biophys. 2009; 46: 172-7, PMID.
Ito F, Sono Y, Ito T. Measurement and clinical significance of lipid peroxidation as a biomarker of oxidative stress: oxidative stress in diabetes, atherosclerosis, and chronic inflammation. Antioxidants. 2019; 8: 72, CrossRef.
Pieme CA, Tatangmo JA, Simo G, Biapa Nya PC, Ama Moor VJ, Moukette Moukette B, et al. Relationship between hyperglycemia, antioxidant capacity and some enzymatic and non-enzymatic antioxidants in African patients with type 2 diabetes. BMC Res Notes. 2017; 10: 141, CrossRef.
Milanlioglu A, Aslan M, Ozkol H, Çilingir V, Nuri Aydın M, Karadas S. Serum antioxidant enzymes activities and oxidative stress levels in patients with acute ischemic stroke: influence on neurological status and outcome. Wien Klin Wochenschr. 2016; 128: 169-74, CrossRef.
Sharebiani H, Fazeli B, Maniscalco R, Ligi D, Mannello F. The imbalance among oxidative biomarkers and antioxidant defense systems in thromboangiitis obliterans (Winiwarter-Buerger Disease). J Clin Med. 2020; 9: 1036, CrossRef.
Yang RL, Shi YH, Hao G, Li W, Le GW. Increasing oxidative stress with progressive hyperlipidemia in human: relation between malondialdehyde and atherogenic index. J Clin Biochem Nutr. 2008; 43: 154-8, CrossRef.
Nita M, Grzybowski A. The role of the reactive oxygen species and oxidative stress in the pathomechanism of the age-related ocular diseases and other pathologies of the anterior and posterior eye segments in adults. Oxid Med Cell Longev. 2016; 2016: 3164734, CrossRef.
Svechnikov K, Landreh L, Weisser J, Izzo G, Colón E, Svechnikov I, et al. Origin, development and regulation of human leydig cells. horm res paediatr. 2010; 73: 93-101, CrossRef.
Permatasari AAAP, Sari NKY, Widhiantara IG. Histopathology of the muscle corpus kavernosum Wistar rat (Rattus norvegicus) hyperlipidemia. Metamorf J Biol Sci. 2018; 5: 254-8, article.
Rosiana IW, Widhiantara IG. Lumen and endotelium penile dorsal arteries hystology of Wistar rats (Rattus novergicus) induced by high-fat diet. Metamorf J Biol Sci. 2020; 7: 73-9, CrossRef.
Rahardjo SS. Review tanaman Sembung [Blumea balsamifera (L.)]. Proc Mulawarman Pharm Conf. 2016; 3: 18-28, CrossRef.
Pang Y, Wang D, Fan Z, Chen X, Yu F, Hu X, et al. Blumea balsamifera—a phytochemical and pharmacological review. Molecules. 2014; 19: 9453-77, CrossRef.
Widhiantara IG, Permatasari AAAP, Wiradana PA. The Effect of Sembung Leaf Extract (Blumea balsamifera) On The Number and Diameter of Rats Leydig Cells Induced By High-Fat Diet. Plant Arch. 2021; 21: 356–61, CrossRef.
Widhiantara IG, Permatasari AAAP, Siswanto FM, Dewi NPES. Sembung (Blumea balsamifera) leaf extract improves testis histology of high-fat diet induced rats. J Bioteknol Biosains Indones. 2018; 5: 111-8, article.
Eriadi A, Uthia R, Novita R. The effect of ethanol extract of Sembung leaves (Blumea balsamifera (L.) DC.) on blood glucose levels and pancreatic histopathology of male white mice induced by alloxan. J Farm Higea. 2017; 9: 127-39.
Ikalinus R, Widyastuti S, Eka Setiasih N. Phytochemical screening ethanol extracts skin stem moringa (Moringa oleifera). Indones Med Veterinus. 2015; 4: 71-9, article.
Hossain MA, AL-Raqmi KAS, AL-Mijizy ZH, Weli AM, Al-Riyami Q. Study of total phenol, flavonoids contents and phytochemical screening of various leaves crude extracts of locally grown Thymus vulgaris. Asian Pac J Trop Biomed. 2013; 3: 705-10, CrossRef.
Love J, Simons CR. Acid hydrolysis of saponins extracted in tincture. PLoS One. 2020; 15: 1-17, CrossRef.
Sri Sulasmi E, Saptasari M, Mawaddah K, Ama Zulfia F. Tannin identification of 4 species pterydophyta from Baluran National Park. J Phys Conf Ser. 2019; 1241: 012002, CrossRef.
Astiti NPA, Sudirga SK, Ramona Y. Analysis of phenolic and tannin contents in the methanol extract of sweet and sour star fruit plants (Averrhoa carambola L) leaves commonly used as raw materials of Lawar (A Balinese Traditional Food). Adv Trop Biodivers Environ Sci. 2019; 3: 5-7, CrossRef.
Ministry of Health Republic of Indonesia. Farmakope Herbal Indonesia Edisi II. Jakarta: Ministry of Health Republic of Indonesia; 2017, article.
Lamont BJ, Waters MF, Andrikopoulos S. A low-carbohydrate high-fat diet increases weight gain and does not improve glucose tolerance, insulin secretion or β-cell mass in NZO mice. Nutr Diabetes. 2016; 6: e194, CrossRef.
Beale KEL, Murphy KG, Harrison EK, Kerton AJ, Ghatei MA, Bloom SR, et al. Accurate measurement of body weight and food intake in environmentally enriched male Wistar rats. Obesity. 2011; 19: 1715-21, CrossRef.
Widhiantara IG, Jawi IM. Phytochemical composition and health properties of Sembung plant (Blumea balsamifera): A review. Vet World. 2021; 14: 1185-96, CrossRef.
Saura-Calixto F, Goñi I. Definition of the mediterranean diet based on bioactive compounds. Crit Rev Food Sci Nutr. 2009; 49: 145-52, CrossRef.
Miller MG, Shukitt-Hale B. Berry fruit enhances beneficial signaling in the brain. J Agric Food Chem. 2012; 60: 5709-15, CrossRef.
Khalilpourfarshbafi M, Gholami K, Murugan DD, Abdul Sattar MZ, Abdullah NA. Differential effects of dietary flavonoids on adipogenesis. Eur J Nutr. 2019; 58: 5-25, CrossRef.
Ngestiningsih D, Rahayu RA, Batubara L. Effect of superoxide dismutase (SOD) supplementation on plasma levels of malondialdehyde (MDA), total cholesterol and LDL cholesterol in the elderly. J Biomed Transl Res. 2019; 5: 29-33, CrossRef.
Mao C, Yuan JQ, Lv YB, Gao X, Yin ZX, Kraus VB, et al. Associations between superoxide dismutase, malondialdehyde and all-cause mortality in older adults: a community-based cohort study. BMC Geriatr. 2019; 19: 104, CrossRef.
Rizal A, Sandra F, Fadlan MR, Sargowo D. Ganoderma lucidum polysaccharide peptide reduce inflammation and oxidative stress in patient with atrial fibrillation. Indones Biomed J. 2020; 12(4): 384-9, CrossRef.
Panche AN, Diwan AD, Chandra SR. Flavonoids: an overview. J Nutr Sci. 2016; 5: e47, CrossRef.
Spiers JG, Chen HJC, Sernia C, Lavidis NA. Activation of the hypothalamic-pituitary-adrenal stress axis induces cellular oxidative stress. Front Neurosci. 2015; 8: 456, CrossRef.
Widhiantara IG, Putri AAA, Permatasari, Rosiana IW, Wiradana PA, Satriyasa BK. Steroidogenesis mechanism, disruption factor, gene function, and role in male fertility : a minirReview. Indian J Forensic Med Toxicol. 2021; 15: 1456-68.
Yang Y, Zhang Z, Li S, Ye X, Li X, He K. Synergy effects of herb extracts: Pharmacokinetics and pharmacodynamic basis. Fitoterapia. 2014; 92: 133-47, CrossRef.
DOI: https://doi.org/10.18585/inabj.v13i4.1694
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