Stenochlaena palustris Ethanol Extract Decreases Viability and Induces G1-Phase Cell Cycle Arrest in HSC-3 Tongue Cancer Cells via p21 and p27
Abstract
BACKGROUND: Oral squamous cell carcinoma (OSCC) of the tongue is an aggressive cancer with a poor prognosis due to its resistance to standard treatments. Stenochlaena palustris, a medicinal fern containing bioactive compounds, has shown potential anticancer properties. However, there is a lack of studies addressing the effects of S. palustris ethanol extract (SPEE) on tongue cancer. This study examined the effects of SPEE on the cell viability and cell cycle of human squamous cell carcinoma (HSC)-3 tongue cancer cells.
METHODS: SPEE was prepared with the maceration method. HSC-3 cells were treated with SPEE at concentrations of 100, 500, and 1000 µg/mL for 24 and 48 hours. Cell viability was measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell cycle analysis was performed using flow cytometer. Immunoblotting was used to measure amount of cell cycle regulators, protein 21 (p21) and protein 27 (p27).
RESULTS: SPEE treatment led to a significant decrease in HSC-3 viable cells in a concentration- and time-dependent manner, with the most pronounced effect at higher concentration and prolonged treatment time. There was a slightly increase in the percentage of cells in the Sub-G1 phase in SPEE-treated group, meanwhile there was a significant increase in the percentage of cells in the G1-phase. Increased amount of p21 and p27 were observed in SPEE-treated group.
CONCLUSION: SPEE significantly inhibited HSC-3 cell proliferation in a concentration- and time-dependent manner, primarily by inducing G1-phase cell cycle arrest through the upregulation of p21 and p27. Taken together, SPEE could be a potential anti-cancer agent for tongue cancer cell.
KEYWORDS: Stenochlaena palustris, tongue cancer, cytotoxic, cell cycle arrest, HSC-3 cells, p21, p27Full Text:
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Migueláñez-Medrán BC, Pozo-Kreilinger JJ, Cebrián-Carretero JL, Martínez-García MA, López-Sánchez AF. Oral squamous cell carcinoma of tongue: Histological risk assessment. A pilot study. Med Oral Patol Oral Cir Bucal. 2019; 24(5): e603-9, CrossRef.
Ginting CN, Lister IN, Girsang E, Riastawati D, Kusuma HS, Widowati W. Antioxidant activities of Ficus elastica leaves ethanol extract and its compounds. Mol Cell Biomed Sci. 2020; 4(1): 27-33, CrossRef.
Sandra F, Hayuningtyas RA, Ranggaini D, Pang T, Scania AE, Lee KH. Elephantopus scaber Linn. leaf extract sensitizes doxorubicin in inducing apoptosis in hsc-3 tongue cancer cells through inhibiting survivin activity at Thr34. Indones Biomed J. 2024; 16(4): 372-8, CrossRef.
Quah Y, Tong SR, Tan SA, Chow YL, Chai TT. Phytochemicals and biological activities of Stenochlaena palustris. In: Murthy HN, editor. Bioactive Compounds in Bryophytes and Pteridophytes. Cham: Springer International Publishing; 2023. p. 503-25, CrossRef.
Ranggaini D, Sandra F, Halim J, Ichwan SJA, Djamil MS. Curcuma xanthorrhiza rhizome extract induces apoptosis in HONE-1 nasopharyngeal cancer cells through bid. Indones Biomed J. 2023; 15(1): 100-5, CrossRef.
Tjajaindra A, Sari AK, Simamora A, Timotius KH. The stem infusate and ethanol extract of physalis angulata inhibitory activities against a-glucosidase and xanthine oxidase. Mol Cell Biomed Sci. 2021; 5(3): 115-20, CrossRef.
Fitriasih F, Komariyah SM, Sandra F, Pratiwi N, Hidayati DN. Mangifera indica L. Leaves extract induced intrinsic apoptotic pathway in MCF-7 cells by decreasing bcl-2 expression and inducing bax expression. Indones J Cancer Chemoprevent. 2019; 10(1): 1-6, CrossRef.
Cicuzza D. Stenochlaena palustris (Burm. f.) Bedd. Blechnaceae. In: Franco FM. Ethnobotany of the Mountain Regions of Southeast Asia. Cham: Springer International Publishing; 2021. p.1021-6, CrossRef.
Debnath SL, Kundu P, Ahad MF, Saha L, Biswas NN, Sadhu SK. Investigation of phytochemical and pharmacological assessment of ethanol extract of Stenochlaena palustris-an edible fern of Sundarbans. J Med Plants Stud. 2021; 9(3): 226-32, CrossRef.
Marisa D, Hayatie L, Juliati S, Suhartono E, Komari N. Molecular docking of phytosterols in Stenochlaena palustris as anti-breast cancer. Acta Bio Ina. 2021; 4(2): 59, CrossRef.
Kashyap D, Garg VK, Goel N. Intrinsic and extrinsic pathways of apoptosis: Role in cancer development and prognosis. Adv Protein Chem Struct Biol. 2021; 125: 73-120, CrossRef.
Widowati W, Jasaputra DK, Sumitro SB, Widodo MA, Afifah E, Rizal R, et al. Direct and indirect effect of TNFα and IFNγ toward apoptosis in breast cancer cells. Mol Cell Biomed Sci. 2018; 2(2): 60-9, CrossRef.
Fu JY, Jiang CX, Wu MY, Mei RY, Yang AF, Tao HP, et al. Theabrownin induces cell apoptosis and cell cycle arrest of oligodendroglioma and astrocytoma in different pathways. Front Pharmacol. 2021; 12: 664003, CrossRef.
Hume S, Grou CP, Lascaux P, D'Angiolella V, Legrand AJ, Ramadan K, et al. The NUCKS1-SKP2-p21/p27 axis controls S phase entry. Nat Commun. 2021; 12(1): 6959, CrossRef.
Abukhdeir AM, Park BH. P21 and p27: Roles in carcinogenesis and drug resistance. Expert Rev Mol Med. 2008; 10: e19, CrossRef.
Mashar HMI, Annah I. Cytotoxicity of kelakai (Stenochlaena palustris) extract to MCF-7 breast cancer cell. J Fitofarmaka Indones. 2020; 7(3): 5-9, CrossRef.
Rahmawati I. Uji Aktivitas sitotoksik herba kelakai (Stenochlaena palustris (Burm. F.) Bedd.) terhadap sel kanker hati HEPG2. J Bioteknol Biosains Indones. 2021; 8(2): 255-66, article.
Fathani IJ, Miladiyah I. Cytotoxicity of ethanolic extract of fruit shells and seeds of Nyamplung (Calophyllum inophyllum L.) on WiDr colorectal cancer cells. J Kedokt Kesehat Indones. 2021; 12 (2): 166-74, CrossRef.
Chear NJ, Fauzi AN, Khaw KY, Choi SB, Yaacob NS, Lai CS. Free radical scavenging and cytotoxic properties of acylated and non-acylated kaempferol glycosides from Stenochlaena palustris: A perspective on their structure-activity relationships. Pharm Chem J. 2019; 53: 188-93, CrossRef.
Pujimulyani D, Yulianto WA, Setyawati A, Rizal R, Qodariah RL, Khoiriyah Z, et al. Curcuma mangga Val. extract as antidiabetic agent in 3T3-L1 adipocyte cells. Mol Cell Biomed Sci. 2020; 4(1): 45-51, CrossRef.
Novilla A, Mustofa M, Astuti I, Jumina J, Suwito H. Cytotoxic activity of methoxy-4'amino chalcone derivatives against leukemia cell lines. Mol Cell Biomed Sci. 2019; 3(1): 34-41, CrossRef.
Milewski D, Pradhan A, Wang X, Cai Y, Le T, Turpin B, et al. FoxF1 and FoxF2 transcription factors synergistically promote rhabdomyosarcoma carcinogenesis by repressing transcription of p21Cip1 CDK inhibitor. Oncogene. 2017; 36(6): 850-62, CrossRef.
Shah S, Mahamat-Saleh Y, Ait-Hadad W, Koemel NA, Varraso R, Boutron-Ruault MC, et al. Long-term adherence to healthful and unhealthful plant-based diets and breast cancer risk overall and by hormone receptor and histologic subtypes among postmenopausal females. Am J Clin Nutr. 2023; 117(3): 467-76, CrossRef.
Utami DT, Nugraheni N, Jenie RI, Meiyanto E. Co-treatment of brazilein enhances cytotoxicity of doxorubicin on WiDr colorectal cancer cells through cell cycle arrest. Indones Biomed J. 2020; 12(4): 376-83, CrossRef.
Valivand N, Aravand S, Lotfi H, Esfahani AJ, Ahmadpour-Yazdi H, Gheibi N. Propolis: A natural compound with potential as an adjuvant in cancer therapy - a review of signaling pathways. Mol Biol Rep. 2024; 51(1): 931, CrossRef.
Karimian H, Moghadamtousi SZ, Fadaeinasab M, Golbabapour S, Razavi M, Hajrezaie M, et al. Ferulago angulata activates intrinsic pathway of apoptosis in MCF-7 cells associated with G1 cell cycle arrest via involvement of p21/p27. Drug Des Devel Ther. 2014; 8: 1481-97, CrossRef.
Choi EJ. Hesperetin induced G1-phase cell cycle arrest in human breast cancer MCF-7 cells: involvement of CDK4 and p21. Nutr Cancer. 2007; 59(1): 115-9, CrossRef.
DOI: https://doi.org/10.18585/inabj.v16i5.3308
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