Lymphocyte Proliferation Kinetics in Inhabitant of Takandeang Village, Mamuju: A High Background Radiation Areas in Indonesia

Dwi Ramadhani; Siti Nurhayati; Tur Rahardjo; Mukh Syaifudin

doi:10.18585/inabj.v10i1.357

Lymphocyte Proliferation Kinetics in Inhabitant of Takandeang Village, Mamuju: A High Background Radiation Areas in Indonesia

Dwi Ramadhani, Siti Nurhayati, Tur Rahardjo, Mukh Syaifudin

Abstract

BACKGROUND: Mamuju area in West Sulawesi considered as the high natural background radiation area in Indonesia. Our previous study showed that the mean mitotic index (MI) and nuclear division index (NDI) in lymphocytes of Botteng Village, Mamuju inhabitants was lower compared to control samples. To validate our previous study results, here in this study the evaluation of cell proliferation markers which were MI and NDI in peripheral blood lymphocytes (PBL) of Takandeang Village inhabitants was conducted.

METHODS: A total 60 people were enrolled in this study, consisted of 35 samples from Takandeang Sub-Village and 25 from normal background radiation area. MI was calculated manually and automatically using Metafer 3.11.2 imaging system. The NDI defined as proportion of mononucleated, binucleated, trinucleated and tetranucleated cells were conducted using cytokinesis block micronucleus (CBMN) assay.

RESULTS: The results of this study showed that the mean manual MI in Takandeang Sub-Village inhabitants was lower compared to control group (4.96±2.25 vs. 5.93±2.14). In contrast, the mean automatic MI (20.37±10.49 vs. 18.87±7.49) and NDI (1.555±0.174 vs. 1.523±0.112) in Takandeang Sub-Village inhabitants was higher compared to the control group. Statistical analysis revealed that the difference of mean manual MI, automatic MI and NDI in Takandeang Sub-Village inhabitants was not significantly different compared to the control group
(p>0.05).

CONCLUSION: It can be concluded that based on this study the chronic low radiation dose exposure in Takandeang Sub-Village, Mamuju has no significant effect on the lymphocytes proliferation.

KEYWORDS: lymphocytes, mitotic index, nuclear division index, high background radiation

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References

Hendry JH, Simon SL, Wojcik A, Sohrabi M, Cardis E, Laurier D, et al. Human exposure to high natural background radiation: what can it teach us about radiation risks? J Radiol Prot. 2009; 29: A29-42, CrossRef.

Mortazavi SMJ, Mozdarani H. Is it time to shed some light on the black box of health policies regarding the inhabitants of the high background radiation areas of ramsar? Iran J Radiat Res. 2012; 10: 111-6, article.

Pourfallah TA, Babapour H, Shahidi M. Evaluation of high level environmental background radiation areas and its variation in ramsar. Iran J Med Phys. 2012; 9: 87-92, article.

Syaeful H, Sukadana IG, Sumaryanto A. Radiometric mapping for naturally occurring radioactive materials (NORM) assessment in Mamuju, West Sulawesi. Atom Indones. 2014; 40: 33-9, CrossRef.

Lubis M, Sardini S, Ramadhani D. Evaluation on mitotic and nuclear division indexes in peripheral blood lymphocytes of Botteng Village, Mamuju inhabitants. In: Suzuki Y, Jeffry RA, Suseno H, Budiawan, Haryanto F, Dumais JR, et al, editors. International Conferences on the Sources, Effects and Risks of Ionizing Radiation 2 (SERIR 2). Sanur, Bali: Center for Technology of Radiation Safety and Metrology; 2016. p.85-9.

Alakoç C, Eroğlu HE. Determining mitotic index in peripheral lymphocytes of welders exposed to metal arc welding fumes. Turk J Biol. 2011; 35: 325-30, CrossRef.

Ionescu ME, Ciocirlan M, Becheanu G, Nicolaie T, Ditescu C, Teiusanu AG, et al. Nuclear division index may predict neoplastic colorectal lesions. Maedica (Buchar). 2011; 6: 173-8, PMID.

Al Amili WA, Hussain NA, Al Faisal AH. Evaluation of micronucleus and nuclear division index in the lymphocytes of some Iraqi patients with acute lymphocyte leukemia. J Biotechnol Res Cent. 2013; 7: 43-53.

UNSCEAR. Report Vol. I Sources and effects of ionizing radiation, annex a: dose assessment methodologies. New York: United Nations Scientific Committee on the effects of atomic radiation; 2000, article.

IAEA. Cytogenetic Dosimetry: Applications In Preparedness For And Response To Radiation Emergencies. Austria: International Atomic Energy Agency; 2011.

UNSCEAR. Sources and Effects of Ionizing Radiation: Sources Annex B. Exposures of the Public and Workers from Various Sources of Radiation. Vol. 1, United Nationas Publications. New York: United Nations Scientific Committee on the effects of atomic radiation; 2008, article.

Fenech MF. The cytokinesis-block micronucleus technique. In: Pfeifer GP, editor. Technologies for Detection of DNA Damage and Mutations. New York : Plenum Press; 1996. p.25-36, CrossRef.

Crespo-lopez ME, Costa-malaquias A, Oliveira EHC, Miranda S, Arrifano GPF, Souza-monteiro JR, et al. Is low non-lethal concentration of methylmercury really safe? A report on genotoxicity with delayed cell proliferation. PLoS One. 2016; 11: 1-14, CrossRef.

Sivikova K, Dianovsky J. Mitotic index and cell proliferation kinetics as additional variables for assessment of genotoxic effect of the herbicide modown. Acta Vet Brno. 2000; 69: 45-50, CrossRef.

Yousif O. Micronucleus analysis and mitotic index in a Jordanian population exposed to pesticides of organophosphate: malathion and chlorpyrifos. Caryologia. 2011; 64: 173-8, CrossRef.

Eroglu HE. The cytogenetic effects of black tea and green tea on cultured human lymphocytes. Brazilian Arch Biol Technol. 2011; 54: 1159-65, CrossRef.

Sinitsky MY, Druzhinin VG. The application of the cytokinesisblock micronucleus assay on peripheral blood lymphocytes for the assessment of genome damage in long-term residents of areas with high radon concentration. J Radiat Res. 2014; 55: 61-6, CrossRef.

Liu SZ, Jin SZ, Liu XD, Sun YM. Role of CD28/B7 costimulation and IL-12/IL-10 interaction in the radiation-induced immune changes. BMC Immunol. 2001; 2: 1-8, CrossRef.

Liu SZ. Nonlinear dose-response relationship in the immune system following exposure to ionizing radiation: mechanisms and implications. Nonlinearity Biol Toxicol Med. 2003; 1: 71-92, CrossRef.

Liu SZ. Radiation-induced change in lymphocyte proliferation and its neuroendocrine regulation: dose-response relationship and pathophysiological implications. Nonlinearity Biol Toxicol Med. 2004; 2: 233-43, CrossRef.

Basri IKH, Yusuf D, Rahardjo T, Nurhayati S, Tetriana D, Ramadhani D, et al. Study of γ-H2AX as DNA double strand break biomarker in resident living in high natural radiation area of Mamuju, West Sulawesi. J Environ Radioact. 2017; 171: 212-6, CrossRef.

Nurhayati S, Purnami S, Syaifudin M. Cytogenetic evaluation in peripheral blood lymphocytes of individuals living in high natural background radiation of Botteng Village, Mamuju. In: Suzuki Y, Jeffry RA, Suseno H, Budiawan, Haryanto F, Dumais JR, et al, editors. International Conferences on the Sources, Effects and Risks of Ionizing Radiation 2 (SERIR 2). Sanur, Bali: Center for Technology of Radiation Safety and Metrology; 2016. p.80-4.

Rahardjo T, Mailana W, Kisnanto T, Darlina D, Nurhayati S, Tetriana D, et al. Assessment of DNA damage in lymphocytes of inhabitants of Mamuju (a high background radiation area) using alkaline single cell gel electrophoresis. Int J Low Radiat. 2017; 10: 314-23, CrossRef.

Ramadhani D, Sardini S, Lubis M, Syaifudin M. Evaluation of lymphocytes proliferation in Botteng village (a high background radiation area) inhabitants using binucleate index. A Sci J Appl Isot Radiat. 2016; 12: 107-12, CrossRef.

Karuppasamy CV, Ramachandran EN, Kumar VA, Kumar PRV, Koya PKM, Jaikrishan G, et al. Peripheral blood lymphocyte micronucleus frequencies in men from areas of Kerala, India, with high vs normal levels of natural background ionizing radiation. Mutat Res - Genet Toxicol Environ Mutagen. 2016; 800- 801: 40-5, CrossRef.

Nefic H, Handzic I. The effect of age, sex, and lifestyle factors on micronucleus frequency in peripheral blood lymphocytes of the Bosnian population. Mutat Res. 2013; 753: 1–11, CrossRef.

Palus J, Rydzynski K, Dziubaltowska E, Wyszynska K, Natarajan AT, Nilsson R. Genotoxic effects of occupational exposure to lead and cadmium. Mutat Res Toxicol Environ Mutagen. 2003; 540: 19-28, CrossRef.

Calderon-Ezquerro C, Guerrero-Guerra C, Sansores-Martinez R, Calderon-Segura ME, Villalobos-Pietrini R, Aamdor-Munoz O, et al. Genotoxicity in lymphocytes of smokers living in Mexico city. Rev Int Contam Ambient. 2010; 26: 47-63, article.

DOI: https://doi.org/10.18585/inabj.v10i1.357

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