Study of Low-grade Chronic Inflammatory Markers in Men with Central Obesity: Cathepsin S was Correlated with Waist Circumference

Adriana Todingrante, Mansyur Arif, Uleng Bahrun, Ferry Sandra


BACKGROUND: There is a prevalence increase of overweight and obesity in Indonesia. Central obesity can lead a variety of chronic diseases through the inflammatory process. There are some markers for low-grade chronic inflammatory, such as cathepsin S, high sensitivity C-reactive protein (hs-CRP), interleukin-1- beta (IL-1β). To our current interest that central obesity can lead to various chronic diseases through the inflammatory process, we conducted a study to investigate correlation of Cathepsin S, hs-CRP, IL-1β in men with central obesity.

METHODS: A cross-sectional study was conducted. Seventy-eight selected subjects were examined to collect anthropometric data and prepared for sample collection. Collected samples were processed for the following biochemical analyses: fasting glucose, high density lipoprotein (HDL)-cholesterol, triglyceride, serum glutamic oxaloacetic transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), cathepsin S, hs-CRP, and IL-1β. Data distribution and variable correlation were then statistically analyzed.

RESULTS: There were significant correlations between waist circumference (WC) and cathepsin S (p=0.030; r=0.214), hs-CRP and cathepsin S (p=0.007; r=0.276), triglyceride and IL-1β (p=0.019; r=-0.235), WC and systolic blood pressure (SBP) (p=0.003; r=-0.312), WC and fasting glucose (p=0.000; r=0.380), WC and body mass index (BMI) (p=0.000; r=0.708).

CONCLUSION: Our study showed that cathepsin S was correlated with central obesity, suggesting that cathepsin S could be a potential inflammatory marker in central obesity in the future.

KEYWORDS: obesity, inflammation, hs-CRP, cathepsin S, IL-1β, waist circumference

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Badan Penelitian dan Pengembangan Kesehatan Departemen Kesehatan RI. Riset Kesehatan Dasar (RISKESDAS) 2007. Jakarta: Depkes RI; 2007.

Shen W, Punyanitya M, Chen J, Gallagher D, Albu J, Pi-Sunyer X, et al. Waist circumference correlates with metabolic syndrome indicators better than percentage fat. Obesity. 2006; 14: 727-36, CrossRef.

Lee CD, Jacobs DR, Schreiner PJ, Iribarren C, Hankinson A. Abdominal obesity and coronary artery calcification in young adults: The coronary artery risk development in young adults (CARDIA) study. Am J Clin Nutr. 2007; 86: 48-54, PMID.

Baik I, Ascherio A, Rimm EB, Giovannucci E, Spiegelman D, Stampfer MJ, et al. Adiposity and mortality in men. Am J Epidemiol. 2000; 152: 264-71, CrossRef.

Wildman RP, Gu D, Reynolds K, Duan X, Wu X, He J. Are waist circumference and body mass index independently associated with cardiovascular disease risk in Chinese adults? Am J Clin Nutr. 2005; 82: 1195-202, PMID.

Wang Y, Rimm EB, Stampfer MJ, Willett WC, Hu FB. Comparison of abdominal adiposity and overall obesity in predicting risk of type 2 diabetes among men. Am J Clin Nutr. 2005; 81: 555-63, PMID.

Krishnan S, Rosenberg L, Djousse L, Cupples LA, Palmer JR. Overall and central obesity and risk of type 2 diabetes in U.S. black woman. Obesity. 2007; 15: 1860-6, CrossRef.

Tsai CJ, Leitzmann MF, Willett WC, Giovannucci EL. Prospective study of abdominal adiposity and gallstone disease in US men. Am J Clin Nutr. 2004; 80: 38-44, PMID.

Chen Y, Rennie D, Cormier YF, Dosman J. Waist circumference is associated with pulmonary function in normal-weight, overweight, and obese subjects. Am J Clin Nutr. 2007; 85: 35-9, PMID.

Barbagallo CM, Cavera G, Sapienza M, Noto D, Cefalu AB, Pagano M, et al. Prevalence of overweight and obesity in a rural southern Italy population and relationships with total and cardiovascular mortality: The ventimiglia di Sicilia project. Int J Obes Relat Metab Disord. 2001; 25: 185-90, CrossRef.

Gnacińska M, Małgorzewicz S, Guzek M, Lysiak-Szydłowska W, Sworczak K. Adipose tissue activity in relation to overweight or obesity. Endokrynol Pol. 2010; 61: 160-8, PMID.

Zhang H, Cui J, Zhang C. Emerging role of adipokines as mediators in atherosclerosis. World J Cardiol. 2010; 2: 370-6, CrossRef.

van Dijk SJ, Feskens EJ, Bos MB, Hoelen DW, Heijligenberg R, Bromhaar MG, et al. A saturated fatty acid-rich diet induces an obesity-linked proinflammatory gene expression profile in adipose tissue of subjects at risk of metabolic syndrome. Am J Clin Nutr. 2009; 90: 1656-64, CrossRef.

Ärnlőv J. Cathepsin S as a biomarker: where are we now and what are the future challenges? Biomark Med. 2012; 6: 9-11, CrossRef.

Naour N, Rouault C, Fellahi S, Lavoie ME, Poitou C, Keophiphath M, et al. Cathepsin in human obesity: changes in energy balance predominantly affect cathepsin S in adipose tissue and in circulation. J Clin Endocrinol Metab. 2010; 95: 1861-8, CrossRef.

Black S, Kushner I, Samols D. C-reactive protein. J Biol Chem. 2004; 279: 48487-90, CrossRef.

Ren K, Torres R. Role of interleukin-1β during pain and inflammation. Brain Res Rev. 2009; 60: 57-4, CrossRef.

Carey N, Lumeng CN, Saltiel AR. Inflammatory links between obesity and metabolic disease. Clin Invest. 2011; 121: 2111-17, CrossRef.

de Nooijer R, Bot I, von der Thusen JH, Leeuwenburgh MA, Overkleeft HS, Kraaijeveld AO, et al. Leukocyte cathepsin S is a potent regulator of both cell and matrix turnover in advanced atherosclerosis. Arterioscler Thromb Vasc Biol. 2009; 29: 188-94, CrossRef.

Chiriboga DE, Ma Y, Li W, Stanek III EJ, bert He´JR, Merriam PA, et al. Seasonal and sex variation of high-sensitivity c-reactive protein in healthy adults: a longitudinal study. Clin Chem. 2009; 55: 313-21, CrossRef.

Jobs E, Rise´rus U, Ingelsson E, Helmersson J, Nerpin E, Jobs M, et al. Serum cathepsin S is associated with serum c-reactive protein and interleukin-6 independently of obesity in elderly male. J Clin Endocrinol Metab. 2010; 95: 4460-4, CrossRef.

Nov O, Shapiro H, Ovadia H, Tarnovscki T, Dvir I, Shemesh E, et al. Interleukin-1β regulates fat-liver crosstalk in obesity by auto-paracrine modulation of adipose tissue inflammation and expandability. PLoS One. 2013; 8: e5362624, CrossRef.

Wellen KE, Hotamisligil GS. Obesity induces inflammatory changes in adipose tissue. J Clin Invest. 2003; 112: 1785-8, CrossRef.

Fain JN. Release of inflammatory mediators by human adipose tissue is enhanced in obesity and primarily by the nonfat cells: A review. Mediators inflamm 2010; 18: 890-96, CrossRef.

Taleb S, Lacasa D, Bastard JP, Poitou C, Cancello R, Pelloux V, et al. Cathepsin S, a novel biomarker of adiposity: relevance to atherogenesis. FASEB J. 2005; 19: 1540-2, CrossRef.

Wang Y, Rimm EB, Stampfer MJ, Willett WC, Hu FB. Comparison of abdominal adiposity and overall obesity in predicting risk of type 2 diabetes among male. Am J Clin Nutr. 2005; 81: 555-63, PMID.

Jung C, Gerdes N, Fritzenwanger M, Figulla RH. Circulating levels of interleukin-1 family cytokines in overweight adolescents. Mediators of Inflamm. 2010; 2010: 958403, CrossRef.

Grant WR, Dixit VD. Mechanisms of disease: inflammasome activation and the development of type 2 diabetes. Front Immunol. 2013; 4: 50, CrossRef.


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