Protective Effect of Curcumin on Quality Parameters of Sperm and Testicular Tissue Alterations in Alloxan-induced Diabetic Rats as Animal Model

Damoon Sadoughi, Mohammad Amin Edalatmanesh, Raheleh Rahbarian

Abstract


BACKGROUND: Type one diabetes mellitus (DM) is a multisystem disorder whose effects are observed in the reproductive system. Considering the role of curcumin in lowering blood glucose and enhancing antioxidant defense, this research aimed to determine protective effect of curcumin on quality parameters of sperm and testicular tissue alterations in diabetic rats.

METHODS: Forty male Wistar rats were randomly divided into five groups, including control (healthy rats), DM (for diabetic model), and also curcumin-treated diabetic model (DM+CUR)50, DM+CUR100 and DM+CUR150. Diabetes was induced using an intraperitoneal injection of 240 mg/ kg alloxan. Following completion of the test period, all rats were sacrificed, and their testes were removed to assess sperm parameters and histological evaluation. The data was analyzed with one-way ANOVA and Tukey post hoc test. The p<0.05 was set as the significance level.

RESULTS: Concentration, total sperm motility (TMot), progressive sperm motility (PMot), average path velocity (VAP), curvilinear velocity (VCL), straight line velocity (VSL) of sperm in DM+CUR100 and DM+CUR150 were significantly increased (p=0.005) compared to the diabetic group. Mean diameter (p=0.004) and thickness of the germline epithelium of testis semeniferous tubules (p=0.005) in DM+CUR100 and DM+CUR150 significantly increased compared to the diabetic group.

CONCLUSION: It can be concluded that curcumin plays a protective role in testicular tissue alterations and improves sperm parameters. In this regard, curcumin could be suggested to be one of the natural antioxidants that can improve fertility in diabetic patients.

KEYWORDS: diabetes mellitus, curcumin, spermatozoa, testis, rats


Full Text:

PDF

References


Zendjabil M. Biological diagnosis of diabetes mellitus. Curr Res Transl Med. 2016: 64: 49-52, CrossRef.

Gaunay G, Nagler HM, Stember DS. Reproductive sequelae of diabetes in male patients. Endocrinol Metab Clin North Am. 2013; 42: 899-914, CrossRef.

Rochette L, Zeller M, Cottin Y, Vergely C. Diabetes, oxidative stress and therapeutic strategies. Biochim Biophys Acta. 2014; 1840: 2709-29, CrossRef.

Asmat U, Abad K, Ismail K. Diabetes mellitus and oxidative stress-A concise review. Saudi Pharm J. 2016; 24: 547-53, CrossRef.

Lushchak VI. Free radicals, reactive oxygen species, oxidative stress and its classification. Chem Biol Interact. 2014; 224: 164-75, CrossRef.

Bjorklund G, Chirumbolo S. Role of oxidative stress and antioxidants in daily nutrition and human health. Nutrition. 2017; 33: 311-21, CrossRef.

Sunagawa Y, Katanasaka Y, Hasegawa K, Morimoto T. Clinical applications of curcumin. Pharma Nutrition. 2015; 3: 131-5, CrossRef.

Salehi B, Stojanović-Radić Z, Matejić J, Sharifi-Rad M, Anil Kumar NV, Martins N, et al. The therapeutic potential of curcumin: A review of clinical trials. Eur J Med Chem. 2019; 163: 527-45, CrossRef.

Soetikno V, Suzuki K, Veeraveedu PT, Arumugam S, Lakshmanan AP, Sone H, et al. Molecular understanding of curcumin in diabetic nephropathy. Drug Discov Today. 2013; 18: 756-63, CrossRef.

Rivera-Mancía S, Trujillo J, Chaverri JP. Utility of curcumin for the treatment of diabetes mellitus: Evidence from preclinical and clinical studies. J Nutr Intermed Metab. 2018; 14: 29-41, CrossRef.

Maithilikarpagaselvi N, Sridhar MG, Swaminathan RP, Zachariah B. Curcumin prevents inflammatory response, oxidative stress and insulin resistance in high fructose fed male Wistar rats: Potential role of serine kinases. Chem Biol Interact. 2016; 244: 187-94, CrossRef.

Głombik K, Basta-Kaim A, Sikora-Polaczek M, Kubera M, Starowicz G, Styrna J. Curcumin influences semen quality parameters and reverses the di(2-ethylhexyl)phthalate (DEHP)-induced testicular damage in mice. Pharmacol Rep. 2014; 66: 782-7, CrossRef.

Zhang L, Diao RY, Duan YG, Yi TH, Cai ZM. In vitro antioxidant effect of curcumin on human sperm quality in leucocytospermia. Andrologia. 2017; 49: 1-6, CrossRef.

Iranloye B, Oghochukwu U. Fertility effects of Curcumin-a kitchen Spice on Male Wistar Rat. Endocrine Abstracts. 2016; 44: 215, CrossRef.

Soleimanzadeh A, Saberivand A. Effect of curcumin on rat sperm morphology after the freeze-thawing process. Vet Res Forum 2013; 4: 185-9, PMID.

Radenković M, Stojanović M, Prostran M. Experimental diabetes induced by alloxan and streptozotocin: The current state of the art. J Pharmacol Toxicol Methods. 2016; 78: 13-31, CrossRef.

Koroglu P, Senturk GE, Yucel D, Ozakpinar OB, Uras F, Arbak S. The effect of exogenous oxytocin on streptozotocin (STZ)-induced diabetic adult rat testes. Peptides. 2015; 63: 47-54, CrossRef.

Ibănescu I, Leiding C, Ciornei ŞG, Roșca P, Sfartz I, Drugociu D. Differences in CASA output according to the chamber type when analyzing frozen-thawed bull sperm. Anim Reprod Sci. 2016; 166: 72-9, CrossRef.

Ezejiofor AN, Orish CN, Orisakwe OE. Cytological and biochemical studies during the progression of alloxan-induced diabetes and possible protection of an aqueous leaf extract of Costus afer. Chin J Nat Med. 2014; 12: 745-52, CrossRef.

Song I, Patel O, Himpe E, Muller CJF, Bouwens L. Beta cell mass restoration in alloxan-diabetic mice treated with EGF and gastrin. PLoS One. 2015; 10: e0140148, CrossRef.

Shoorei H, Khaki A, Khaki AA, Hemmati AA, Moghimian M, Shokoohi M. The ameliorative effect of carvacrol on oxidative stress and germ cell apoptosis in testicular tissue of adult diabetic rats. Biomed Pharmacother. 2018; 111: 568-78, CrossRef.

Bhattacharya SM, Ghosh M, Nandi N. Diabetes mellitus and abnormalities in semen analysis. J Obstet Gynaecol Res. 2014; 40: 167-71, CrossRef.

Roy VK, Chenkual L, Gurusubramanian G. Protection of testis through antioxidant action of Mallotus roxburghianus in alloxaninduced diabetic rat model. J Ethnopharmacol. 2015; 176: 268-80, CrossRef.

Kianifard D, Sadrkhanlou R, Hasanzadeh S. The histological, histomorphometrical and histochemical changes of testicular tissue in the metformin treated and untreated streptozotocin-induced adult diabetic rats. Vet Res Forum. 2011; 2: 13-24, article.

Kanter M, Aktas C, Erboga M. Curcumin attenuates testicular damage, apoptotic germ cell death, and oxidative stress in streptozotocininduced diabetic rats. Mol Nutr Food Res. 2013; 57: 1578-85, CrossRef.

Condorelli RA, Vignera SL, Mongioì LM, Alamo A, Calogero AE. Diabetes mellitus and infertility: different pathophysiological effects in type 1 and type 2 on sperm function. Front Endocrinol. 2018; 9: 1-9, CrossRef.

Ghosh S, Banerjee S, Sil PC. The beneficial role of curcumin on inflammation, diabetes and neurodegenerative disease: a recent update. Food Chem Toxicol. 2015; 83: 111-24, CrossRef.

Zhang DW, Fu M, Gao SH, Liu JL. Curcumin and diabetes: a systematic review. Evid Based Complement Alternat Med. 2013; 2013: 636053, CrossRef.

El-Azab MF, Attia FM, El-Mowafy AM. Novel role of curcumin combined with bone marrow transplantation in reversing experimental diabetes: Effects on pancreatic islet regeneration, oxidative stress, and inflammatory cytokines. Eur J Pharmacol. 2011; 658: 41-8, CrossRef.

Rivera-Mancía S, Lozada-García MC, Pedraza-Chaverri J. Experimental evidence for curcumin and its analogs for management of diabetes mellitus and its associated complications. Eur J Pharmacol. 2015; 756: 30-7, CrossRef.

Murphy CJ, Tang H, Van Kirk EA, Shen Y, Murdoch WJ. Reproductive effects of a pegylated curcumin. Reprod Toxicol. 2012; 34: 120-4, CrossRef.

Elballat SE. Protective effect of curcumin and vitamin C each alone and in combination on cisplatin-induced sperm abnormalities in male albino rats. JOBAZ. 2016; 76: 52-9, CrossRef.

Zha W, Bai Y, Xu L, Liu Y, Yang Z, Gao H, et al. Curcumin attenuates testicular injury in rats with streptozotocin-induced diabetes. Biomed Res Int. 2018; 2018: 7468019, CrossRef.

Lee A, Lee S, Lee S, Yang B. Effects of curcumin on sperm motility, viability, mitochondrial activity and plasma membrane integrity in boar semen. Biomed Sci Letters. 2017; 23: 406-10, CrossRef.

Lu WP, Mei XT, Wang Y, Zheng YP, Xue YF, Xu DH. Zn (II)-curcumin protects against oxidative stress, deleterious changes in sperm parameters and histological alterations in a male mouse model of cyclophosphamide-induced reproductive damage. Environ Toxicol Pharmacol. 2015; 39: 515-24, CrossRef.

Yan W, Kanno C, Oshima E, Kuzuma Y, Kim SW, Bai H. Enhancement of sperm motility and viability by turmeric by-product dietary supplementation in roosters. Anim Reprod Sci. 2017; 185: 195-204, CrossRef.

Tvrdá E, Tušimová E, Kováčik A, Paál D, Greifová H, Abdramanov A, et al. Curcumin has protective and antioxidant properties on bull spermatozoa subjected to induced oxidative stress. Anim Reprod Sci. 2016; 172: 10-20, CrossRef.

Rashid K, Sil PC. Curcumin ameliorates testicular damage in diabetic rats by suppressing cellular stress-mediated mitochondria and endoplasmic reticulum-dependent apoptotic death. Biochim Biophys Acta. 2015; 1852: 70-82, CrossRef.

Chandra AK, Chatterjee A, Ghosh R, Sarkar M. Effect of curcumin on chromium-induced oxidative damage in male reproductive system. Environ Toxicol Pharmacol. 2007; 24: 160-6, CrossRef.




DOI: https://doi.org/10.18585/inabj.v11i3.733

Indexed by:

                 

                  

               

     

 

The Prodia Education and Research Institute