Momordica charantia L. Fruit Fractions inhibit Malondialdehyde Level and Regenerate Hepatic Damage of Hyperglycemic Rats

Parawansah Parawansah, I Putu Sudayasa, Andi Noor Kholidha Syarifin, Amirudin Eso, Nuralifah Nuralifah, Wa Ode Siti Rahayu Fathanah, Ferry Sandra


BACKGROUND: Chronic hyperglycemia causes an increase of free radical production and in longterm, the hyperglycemia increases oxidative stress. Among medicinal plants, Momordica charantia L. fruit has been known to overcome hyperglycemia. However, role of M. charantia L. fruit on oxidative stress is not well understood. Therefore, current study was conducted to investigate the effect of M. charantia L. fruit extract on malondialdehyde (MDA) level and hepatic damage in hyperglicemic rat model.

METHODS: Twenty five white rats (Rattus novergicus) were induced with Streptozotocin (STZ) and treated with/without glibenclamide, sodium carboxymethyl cellulose (Na-CMC), or M. charantia L. fruit ethanol/ethyl acetate/ n-hexane fraction. After the treatment, rat’s livers were collected and separated for histopathological examination and MDA analysis.

RESULTS: The MDA level average of rats before the STZ induction was 1.37 μg/mL. MDA level average was markedly increased (23.85 μg/mL) in rats induced with STZ and treated with Na-CMC merely. The MDA level average of STZ-induced glibenclamide-treated rats was 3.12 μg/mL. Meanwhile, the MDA level averages of STZ-induced M. charantia L. fruit ethanol, ethyl acetate and n-hexane fractions-treated rats were 14.95, 8.98 and 5.37 μg/mL, respectively. The histopathology results of this study showed that adipocytes, dilated sinusoids and central vein thickening were mostly observed in STZ-induced Na-CMC-treated rats. Meanwhile, the STZ-induced ethanol/ethyl acetate/n-hexane fraction-treated rats did not exhibitthose expressions.

CONCLUSION: M. charantia L. fruit fractions inhibit the MDA level average in liver tissue and regenerate hepatic damage of STZ-induced rats, especially the n-hexane fraction which could be a potential hepatic antioxidant and regenerative agent.

KEYWORDS: Momordica charantia L., malondialdehyde, oxidative stress, hyperglycemia, diabetes mellitus

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Widowati W, Maesaroh, Fauziah N, Erawijantari PP, Sandra F. Free Radical Scavenging and α-/β-glucosidase Inhibitory Activities of Rambutan (Nephelium lappaceum L.) Peel Extract. Indones Biomed J. 2015; 7: 157-62, CrossRef.

Hamuaty RB, Sukmawati IR, Sandra F. Relationship between sRAGE and hsCRP as Markers of Cardiovascular Disease Risk Factors in Diabetic and Non-Diabetic Men with Central Obesity. Mol Cell Biomed Sci. 2017; 1: 70-4, CrossRef.

International Diabetes Federation. IDF Diabetes Atlas. 9th ed. [internet]. Brussels: International Diabetes Federation; 2019. [cited: 2019 Nov 27]. Available at:

Sharma P, Jha AB, Dubey RS, Pessarakli M. Reactive Oxygen Species, Oxidative Damage, and Antioxidative Defense Mechanism in Plants under Stressful Conditions. J Botany. 2012; 2012: 217037, CrossRef.

Goudu AS, Naidu MD, Pradesh A. Effect of Fluoride on Oxidative Stress and Biochemical Markers of Bone Turnover in Postmenopausal Women. Fluoride. 2013; 46: 208-11, article.

Sheikhpour R. Diabetes and Oxidative Stress: The Mechanism and Action. Iranian J Diabetes Obesity. 2013; 5: 40-5, article.

Singh UN, Kumar S, Dhakal S. Study of Oxidative Stress in Hypercholesterolemia. Int J Contemp Med Res. 2017; 4: 1204-7, article.

Tangvarasittichai S, Poonsub P, Tangvarasittichai O, Sirigulsatien V. Serum Levels of Malondialdehyde in Type 2 Diabetes Mellitus Thai Subjects. Siriraj Med J. 2009; 6: 20-3, article.

Grotto D, Maria LS, Valentini J, Paniz C, Schmitt G, Garcia S, et al. Importance of The Lipid Peroxidation Biomarkers and Methodological Aspects for Malondialdehyde Quantification. Quim. Nova. 2009; 32: 169-74, CrossRef.

Parawansah, Nuralifah, Alam G, Natzir R. Inhibition of Xanthine Oxidase Activity by Ethanolic Extract of Piperomia pellucids L., Acalypha indica L., and Momordica charantia L. Indones Biomed J. 2016; 8: 161-6, CrossRef.

Weitner T, Inić S, Jablan J, Gabričević M, Domijan AM. Spectrophotometric Determination of Malondialdehyde in Urine Suitable for Epidemiological Studies. Croat Chem Acta. 2016; 89: 133-9, CrossRef.

Jalees SS, Rosaline M. Study of Malondialdehyde and Estimation of Blood Glucose Levels in Patients with Diabetes Mellitus with Cataract. Int J Clin Biochem Res. 2017; 4: 319-23, article.

Forbes JM, Cooper ME. Mechanisms of Diabetic Complications. Physiol Rev. 2013; 93: 137-88, CrossRef.

American Diabetes Association. Microvascular Complications and Foot Care: Standards of Medical Care in Diabetes 2018. Diabetes Care. 2018; 41: S105-18, CrossRef.

Efird JT, Choi YM, Davies SW, Mehra S, Anderson EJ, Katunga LA. Potential for Improved Glycemic Control with Dietary Momordica charantia L. in Patients with Insulin Resistance and Pre-Diabetes. Int J Environ Res Public Health. 2014; 11: 2328-45, CrossRef.

Joseph B, Jini D. Antidiabetic Effects of Momordica charantia (Bitter melon) and Its Medicinal Potency. Asian Pac J Trop Dis. 2013; 3: 93-102, CrossRef.

Pinheiro L, de Melo AD, Andreazzi AE, de Caires Júnior LC, Costa MB, Garcia RMG. Protocol of Insulin Therapy for Streptozotocin-Diabetic Rats Based on a Study of Food Ingestion and Glycemic Variation. Scand J Lab Anim Sci. 2011; 38: 117-27, article.

Szkudelski T. The Mechanism of Alloxan and Streptozotocin Action in β Cells of the Rat Pancreas. Physiol. Res. 2001; 50: 537-46, PMID.

Erejuwa OO, Sulaiman SA, Wahab MSA, Salam SK, Salleh MS, Gurtu S. Comparison of Antioxidant Effects of Honey, Glibenclamide, Metformin, and Their Combinations in the Kidneys of Streptozotocin-Induced Diabetic Rats. Int J Mol Sci. 2011; 12: 829-43, CrossRef.

Singh SR, Hijam D, Dubey A, Devi NO, Jamir S, Longkumer C, et al. Study of Oxidative Stress Status in Type 2 Diabetic Patients. Int J Contemp Med Res. 2015; 2: 20-6, article.

Nagappan, Krishnaveni., Anoop, Karthika, Kowmudi, Gullapalli, Sailaja, Mukkamala. Charantin: A Neglected Antidiabetic Compound from Momordica charantia L. Int J Pharm Sci Rev Res. 2018; 51: 35-40, article.

Altinterim B. Bitter Melon (Momordica charantia) and the Effects of Diabetes Disease. J Agricultur Fac Uludag Univ. 2012; 26: 65-9, article.

Parawansah, Nurtamin T, Mulyawati SA, Nuralifah, Misnaeni, WOA. Immunomodulatory Effect of Momordica charantia L. Fruit Ethanol Extract on Phagocytic Activity and Capacity of Mice Peritoneal Macrophages. Indones Biomed J. 2018; 10: 144-7, CrossRef.

Arsad SS, Esa NM, Hamzah H. Histopathologic Changes in Liver and Kidney Tissues from Male Sprague Dawley Rats Treated with Rhaphidophora decursiva (Roxb.) Schott Extract. J Cytol Histol. 2014; S4: 001, CrossRef.

Zafar M, Naeem-Ul-Hassan Naqvi S, Ahmed M, Kaimkhani ZA. Altered Liver Morphology and Enzymes in Streptozotocin Induced Diabetic Rats. Int J Morphol. 2009; 27: 719-25, CrossRef.


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