Phylogeny of HPV-16 and HPV-18 Multiple Infection of a Patient with Cervical Cancer from Dr. Hasan Sadikin General Hospital, Bandung: A Case Report

Vera Amalia Lestari; Ika Agus Rini; Gita Widya Pradini; Edhyana Sahiratmadja; Herman Susanto

doi:10.18585/inabj.v10i3.383

Phylogeny of HPV-16 and HPV-18 Multiple Infection of a Patient with Cervical Cancer from Dr. Hasan Sadikin General Hospital, Bandung: A Case Report

Vera Amalia Lestari, Ika Agus Rini, Gita Widya Pradini, Edhyana Sahiratmadja, Herman Susanto

Abstract

BACKGROUND: From all of human papillomaviruses (HPV) genotypes capable of causing cervical cancer, it is estimated that 70 percent are HPV-16 and HPV-18. HPV-16 can infect the tissues in single infection or together with other high-risk types of HPV, and the most common is with HPV-18. The origin of HPV can be identified by its phylogenetic tree. The aim of this study was to determine the phylogeny of HPV-16 and HPV-18 multiple infection in cervical cancer, whether both HPVs were from the same origin.

METHODS: Cervical tissue biopsies (n=33) were obtained from Hasan Sadikin Hospital in the period of September to November 2016. HPV genotyping test was performed to confirm the HPV-16 and HPV-18 multiple infection. L1 gene of both HPV-16 and HPV-18 were sequenced for phylogenetic analysis.

RESULTS: Phylogenetic analysis of L1 HPV-16 and HPV-18 showed the closest relationship with sequence from China and Thailand, respectively.

CONCLUSION: HPV-16 and HPV-18 multiple infection of a cervical cancer patient from Dr. Hasan Sadikin General Hospital Bandung showed a very close L1 phylogeny relationship with isolate from Asian region.

KEYWORDS: HPV-16, HPV-18, multiple infection, cervical cancer, Bandung

Full Text:

PDF

References

Pusat Data dan Informasi Depkes RI. Situasi Penyakit Kanker. Jakarta: Kementrian Kesehatan RI; 2015.

Vet JN, de Boer MA, van den Akker BE, Siregar B, Lisnawati, Budiningsih S, et al. Prevalence of human papillomavirus in Indonesia: a population-based study in three regions. Br J Cancer. 2008; 99: 214-8, CrossRef.

Sahiratmadja E, Tobing MDL, Dewayani BM, Hernowo BS, Susanto H. Multiple human papilloma virus infections predominant in squamous cell cervical carcinoma in Bandung. Univ Med. 2014; 33: 58-64, article.

Alba A, Cararach M, Rodriguez-Cerdeira C. The human papillomavirus (HPV) in human pathology: description, pathogenesis, oncogenic role, epidemiology and detection techniques. Dermatol. J. 2009; 3: 90-102, CrossRef.

Doorbar J, Egawa N, Grif H, Kranjec C, Murakami I. Human papillomavirus molecular biology and disease association. Rev Med Virol. 2015; 25: 2-23, CrossRef.

Handisurya A, Schellenbacher C, Kirnbauer R. Diseases caused by human papillomaviruses (HPV). 7: 453-66, CrossRef.

Joura EA, Giuliano AR, Iversen OE, Bouchard C, Mao C, Mehlsen J, et al. A 9-valent HPV vaccine against infection and intraepithelial neoplasia in women. N Engl J Med. 2015; 372: 711-23, CrossRef.

Dwipoyono B. Kanker serviks dan vaksin HPV. Indones J Cancer. 2007; 3: 87-91, article.

Burk RD, Harari A, Chen Z. Human papillomavirus genome variants. Virol. 2013; 445: 232-43, CrossRef.

Sichero I, Villa LL. Epidemiological and functional implication of molecular variant of human papillomavirus. Braz J Med Biol Res. 2006; 39: 700-17, CrossRef.

Gurgel APAD, Chagas BS, do Amaral CM, Nascimento KCG, Leal LRS, Neto JDCS, et al. Prevalence of human papillomavirus variants and genetic diversity in the L1 gene and long control region of HPV16, HPV31, and HPV58 found in North-East Brazil. Biomed Res Int. 2015; 2015: 130828, CrossRef.

Cornet I, Gheit T, Franceschi S, Vignat J, Burk RD, Sylla BS, et al. Human papillomavirus type 16 genetic variants: phylogeny and classification based on E6 and LCR. J Virol. 2012; 86: 6855-61, CrossRef.

Lavezzo E, Masi G, Toppo S, Franchin E, Gazzola V, Sinigaglia A, et al. Characterization of intra-type variants of oncogenic human papillomaviruses by next-generation deep sequencing of the E6/E7 region. Viruses. 2016; 8: 79, CrossRef.

Chen Z, Schiffman M, Herrero R, Desalle R, Anastos K, Segondy M, et al. Evolution and taxonomic classification of human papillomavirus 16 (HPV16)-related variant genomes. PlosOne. 2011; 6: 1-16, CrossRef.

Chen Z, DeSalle R, Schiffman M, Herrero R, Burk RD. Evolutionary dynamics of variant genomes of human papillomavirus types 18, 45, and 97. J Virol. 2009; 83: 1443-55, CrossRef.

Chen Z, Terai M, Fu L, Herrero R, DeSalle R, Burk RD. Diversifying selection in human papillomavirus type 16 lineages based on complete genome analyses. J Virol. 2005; 79: 7014-23, CrossRef.

Fadhilah FR, Sahiratmadja E, Safitri R, Maskoen AM, Susanto H. Analisis filogenetik gen L1 human papillomavirus 16 pada penderita kanker serviks di Bandung. MKB. 2013; 47: 174-8, CrossRef.

Chen AA, Gheit T, Franceschi S, Tommasino M, Clifford GM. Human papillomavirus 18 Genetic Variation and Cervical Cancer Risk Worldwide. J Virol. 2015: 89: 10680-7, CrossRef.

Kodaman N, Sobota RS, Mera R, Schneider BG, Williams SM. Disrupted human–pathogen co-evolution: a model for disease. Frontiers in Genetics. 2014; 5: 290, CrossRef.

Cornet I, Gheit T, Iannacone MR, Vignat J, Sylla BS, Del Mistro A, et al. HPV16 genetic variation and the development of cervical cancer worldwide. Br J Cancer. 2013; 108: 240-44, CrossRef.

Panigoro R, Susanto H, Novel SS, Hartini S, Sahiratmadja E. HPV genotyping linear assay test comparison in cervical cancer patients: implications for HPV prevalence and molecular epidemiology in a limited-resource area in Bandung, Indonesia. Asian Pac J Cancer Prev. 2013; 14: 5843-7, CrossRef.

DOI: https://doi.org/10.18585/inabj.v10i3.383

Copyright (c) 2018 The Prodia Education and Research Institute